Experiment: R2A with 3-octanone 5.31033333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS11375 and ABIE41_RS11380 overlap by 4 nucleotides ABIE41_RS11380 and ABIE41_RS11385 are separated by 78 nucleotides ABIE41_RS11385 and ABIE41_RS11390 overlap by 12 nucleotides
ABIE41_RS11375: ABIE41_RS11375 - methyl-accepting chemotaxis protein, at 2,335,689 to 2,337,686
_RS11375
ABIE41_RS11380: ABIE41_RS11380 - tRNA-binding protein, at 2,337,683 to 2,338,051
_RS11380
ABIE41_RS11385: ABIE41_RS11385 - histidine kinase dimerization/phosphoacceptor domain -containing protein, at 2,338,130 to 2,339,344
_RS11385
ABIE41_RS11390: ABIE41_RS11390 - ATP-binding protein, at 2,339,333 to 2,340,754
_RS11390
Position (kb)
2338
2339
2340 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2337.816 kb on + strand, within ABIE41_RS11380 at 2337.816 kb on + strand, within ABIE41_RS11380 at 2337.878 kb on + strand, within ABIE41_RS11380 at 2337.878 kb on + strand, within ABIE41_RS11380 at 2337.879 kb on - strand, within ABIE41_RS11380 at 2337.879 kb on - strand, within ABIE41_RS11380 at 2338.046 kb on + strand at 2338.046 kb on + strand at 2338.046 kb on + strand at 2338.046 kb on + strand at 2338.046 kb on + strand at 2338.046 kb on + strand at 2338.047 kb on - strand at 2338.047 kb on - strand at 2338.047 kb on - strand at 2338.047 kb on - strand at 2338.047 kb on - strand at 2338.047 kb on - strand at 2338.096 kb on - strand at 2338.193 kb on + strand at 2338.313 kb on + strand, within ABIE41_RS11385 at 2338.520 kb on + strand, within ABIE41_RS11385 at 2338.643 kb on - strand, within ABIE41_RS11385 at 2338.647 kb on - strand, within ABIE41_RS11385 at 2338.647 kb on - strand, within ABIE41_RS11385
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.31033333333333 mM remove 2,337,816 + ABIE41_RS11380 0.36 -0.5 2,337,816 + ABIE41_RS11380 0.36 -0.2 2,337,878 + ABIE41_RS11380 0.53 +1.2 2,337,878 + ABIE41_RS11380 0.53 +0.4 2,337,879 - ABIE41_RS11380 0.53 +0.5 2,337,879 - ABIE41_RS11380 0.53 +0.6 2,338,046 + +0.5 2,338,046 + +1.5 2,338,046 + +1.1 2,338,046 + +0.7 2,338,046 + -0.0 2,338,046 + -2.0 2,338,047 - +0.5 2,338,047 - -0.1 2,338,047 - -0.3 2,338,047 - -0.3 2,338,047 - +2.6 2,338,047 - -0.3 2,338,096 - -0.2 2,338,193 + +2.0 2,338,313 + ABIE41_RS11385 0.15 -0.6 2,338,520 + ABIE41_RS11385 0.32 -2.4 2,338,643 - ABIE41_RS11385 0.42 +0.6 2,338,647 - ABIE41_RS11385 0.43 -2.8 2,338,647 - ABIE41_RS11385 0.43 -0.1
Or see this region's nucleotide sequence