Experiment: R2A with 3-octanone 5.31033333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS07790 and ABIE41_RS07795 are separated by 3 nucleotides ABIE41_RS07795 and ABIE41_RS07800 are separated by 115 nucleotides ABIE41_RS07800 and ABIE41_RS07805 are separated by 158 nucleotides
ABIE41_RS07790: ABIE41_RS07790 - DUF808 domain-containing protein, at 1,599,978 to 1,600,934
_RS07790
ABIE41_RS07795: ABIE41_RS07795 - nitroreductase, at 1,600,938 to 1,601,618
_RS07795
ABIE41_RS07800: ABIE41_RS07800 - lysine--tRNA ligase, at 1,601,734 to 1,603,443
_RS07800
ABIE41_RS07805: ABIE41_RS07805 - amidohydrolase, at 1,603,602 to 1,604,765
_RS07805
Position (kb)
1601
1602
1603
1604 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1600.893 kb on + strand at 1600.894 kb on - strand at 1600.986 kb on + strand at 1601.022 kb on + strand, within ABIE41_RS07795 at 1601.022 kb on + strand, within ABIE41_RS07795 at 1601.023 kb on - strand, within ABIE41_RS07795 at 1601.023 kb on - strand, within ABIE41_RS07795 at 1601.023 kb on - strand, within ABIE41_RS07795 at 1601.023 kb on - strand, within ABIE41_RS07795 at 1601.170 kb on - strand, within ABIE41_RS07795 at 1601.308 kb on + strand, within ABIE41_RS07795 at 1601.344 kb on + strand, within ABIE41_RS07795 at 1601.345 kb on - strand, within ABIE41_RS07795 at 1601.345 kb on - strand, within ABIE41_RS07795 at 1601.368 kb on + strand, within ABIE41_RS07795 at 1601.371 kb on + strand, within ABIE41_RS07795 at 1601.371 kb on + strand, within ABIE41_RS07795 at 1601.377 kb on + strand, within ABIE41_RS07795 at 1601.378 kb on - strand, within ABIE41_RS07795 at 1601.378 kb on - strand, within ABIE41_RS07795 at 1601.527 kb on + strand, within ABIE41_RS07795 at 1603.562 kb on + strand at 1603.563 kb on - strand at 1603.564 kb on + strand at 1603.565 kb on - strand at 1603.565 kb on - strand at 1603.565 kb on - strand at 1603.565 kb on - strand at 1603.565 kb on - strand at 1603.565 kb on - strand at 1603.565 kb on - strand at 1603.565 kb on - strand at 1603.854 kb on + strand, within ABIE41_RS07805 at 1603.854 kb on + strand, within ABIE41_RS07805 at 1603.854 kb on + strand, within ABIE41_RS07805 at 1603.854 kb on + strand, within ABIE41_RS07805 at 1603.854 kb on + strand, within ABIE41_RS07805 at 1604.259 kb on - strand, within ABIE41_RS07805 at 1604.260 kb on + strand, within ABIE41_RS07805
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.31033333333333 mM remove 1,600,893 + -1.3 1,600,894 - -0.3 1,600,986 + -0.9 1,601,022 + ABIE41_RS07795 0.12 +0.4 1,601,022 + ABIE41_RS07795 0.12 +0.5 1,601,023 - ABIE41_RS07795 0.12 +1.4 1,601,023 - ABIE41_RS07795 0.12 -0.0 1,601,023 - ABIE41_RS07795 0.12 +0.4 1,601,023 - ABIE41_RS07795 0.12 -1.6 1,601,170 - ABIE41_RS07795 0.34 -2.4 1,601,308 + ABIE41_RS07795 0.54 -0.2 1,601,344 + ABIE41_RS07795 0.60 -1.0 1,601,345 - ABIE41_RS07795 0.60 +0.3 1,601,345 - ABIE41_RS07795 0.60 -1.9 1,601,368 + ABIE41_RS07795 0.63 -2.0 1,601,371 + ABIE41_RS07795 0.64 +0.4 1,601,371 + ABIE41_RS07795 0.64 +0.2 1,601,377 + ABIE41_RS07795 0.64 -0.5 1,601,378 - ABIE41_RS07795 0.65 +2.1 1,601,378 - ABIE41_RS07795 0.65 -1.7 1,601,527 + ABIE41_RS07795 0.86 -1.8 1,603,562 + +0.3 1,603,563 - +2.1 1,603,564 + +0.5 1,603,565 - -0.2 1,603,565 - +1.3 1,603,565 - +0.0 1,603,565 - -0.2 1,603,565 - +0.3 1,603,565 - +0.5 1,603,565 - -1.1 1,603,565 - +1.4 1,603,854 + ABIE41_RS07805 0.22 +1.3 1,603,854 + ABIE41_RS07805 0.22 -0.1 1,603,854 + ABIE41_RS07805 0.22 -0.5 1,603,854 + ABIE41_RS07805 0.22 -2.2 1,603,854 + ABIE41_RS07805 0.22 -5.4 1,604,259 - ABIE41_RS07805 0.56 +0.1 1,604,260 + ABIE41_RS07805 0.57 +0.1
Or see this region's nucleotide sequence