Experiment: R2A with 3-octanone 5.31033333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS05715 and ABIE41_RS05720 are separated by 54 nucleotides ABIE41_RS05720 and ABIE41_RS05725 are separated by 115 nucleotides ABIE41_RS05725 and ABIE41_RS05730 overlap by 4 nucleotides
ABIE41_RS05715: ABIE41_RS05715 - efflux RND transporter periplasmic adaptor subunit, at 1,175,792 to 1,176,961
_RS05715
ABIE41_RS05720: ABIE41_RS05720 - TetR/AcrR family transcriptional regulator, at 1,177,016 to 1,177,789
_RS05720
ABIE41_RS05725: ABIE41_RS05725 - glutamine amidotransferase, at 1,177,905 to 1,178,696
_RS05725
ABIE41_RS05730: ABIE41_RS05730 - FAD-dependent oxidoreductase, at 1,178,693 to 1,179,934
_RS05730
Position (kb)
1177
1178 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1176.495 kb on + strand, within ABIE41_RS05715 at 1176.495 kb on + strand, within ABIE41_RS05715 at 1176.495 kb on + strand, within ABIE41_RS05715 at 1176.495 kb on + strand, within ABIE41_RS05715 at 1176.495 kb on + strand, within ABIE41_RS05715 at 1176.495 kb on + strand, within ABIE41_RS05715 at 1176.495 kb on + strand, within ABIE41_RS05715 at 1176.495 kb on + strand, within ABIE41_RS05715 at 1176.496 kb on - strand, within ABIE41_RS05715 at 1176.496 kb on - strand, within ABIE41_RS05715 at 1176.496 kb on - strand, within ABIE41_RS05715 at 1176.496 kb on - strand, within ABIE41_RS05715 at 1176.496 kb on - strand, within ABIE41_RS05715 at 1177.021 kb on + strand at 1177.022 kb on + strand at 1177.022 kb on + strand at 1177.022 kb on + strand at 1177.022 kb on + strand at 1177.022 kb on + strand at 1177.022 kb on + strand at 1177.022 kb on + strand at 1177.022 kb on + strand at 1177.022 kb on + strand at 1177.022 kb on + strand at 1177.022 kb on + strand at 1177.023 kb on - strand at 1177.023 kb on - strand at 1177.023 kb on - strand at 1177.023 kb on - strand at 1177.023 kb on - strand at 1177.023 kb on - strand at 1177.023 kb on - strand at 1177.023 kb on - strand at 1177.023 kb on - strand at 1177.488 kb on + strand, within ABIE41_RS05720 at 1177.489 kb on - strand, within ABIE41_RS05720 at 1177.489 kb on - strand, within ABIE41_RS05720 at 1177.494 kb on + strand, within ABIE41_RS05720 at 1178.224 kb on - strand, within ABIE41_RS05725 at 1178.280 kb on + strand, within ABIE41_RS05725 at 1178.281 kb on - strand, within ABIE41_RS05725 at 1178.281 kb on - strand, within ABIE41_RS05725 at 1178.282 kb on + strand, within ABIE41_RS05725 at 1178.282 kb on + strand, within ABIE41_RS05725 at 1178.283 kb on - strand, within ABIE41_RS05725 at 1178.317 kb on - strand, within ABIE41_RS05725 at 1178.388 kb on + strand, within ABIE41_RS05725 at 1178.388 kb on + strand, within ABIE41_RS05725 at 1178.388 kb on + strand, within ABIE41_RS05725 at 1178.388 kb on + strand, within ABIE41_RS05725 at 1178.388 kb on + strand, within ABIE41_RS05725 at 1178.388 kb on + strand, within ABIE41_RS05725 at 1178.389 kb on - strand, within ABIE41_RS05725 at 1178.389 kb on - strand, within ABIE41_RS05725 at 1178.491 kb on - strand, within ABIE41_RS05725 at 1178.491 kb on - strand, within ABIE41_RS05725 at 1178.610 kb on + strand, within ABIE41_RS05725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.31033333333333 mM remove 1,176,495 + ABIE41_RS05715 0.60 -0.5 1,176,495 + ABIE41_RS05715 0.60 +1.5 1,176,495 + ABIE41_RS05715 0.60 +0.9 1,176,495 + ABIE41_RS05715 0.60 +0.3 1,176,495 + ABIE41_RS05715 0.60 -1.8 1,176,495 + ABIE41_RS05715 0.60 -0.5 1,176,495 + ABIE41_RS05715 0.60 +0.3 1,176,495 + ABIE41_RS05715 0.60 -0.9 1,176,496 - ABIE41_RS05715 0.60 -1.8 1,176,496 - ABIE41_RS05715 0.60 -0.2 1,176,496 - ABIE41_RS05715 0.60 -0.8 1,176,496 - ABIE41_RS05715 0.60 +0.2 1,176,496 - ABIE41_RS05715 0.60 -0.4 1,177,021 + +1.0 1,177,022 + +1.1 1,177,022 + -1.7 1,177,022 + -1.4 1,177,022 + -0.5 1,177,022 + +0.5 1,177,022 + -1.1 1,177,022 + -1.0 1,177,022 + -1.3 1,177,022 + +0.3 1,177,022 + -0.6 1,177,022 + +0.1 1,177,023 - -0.2 1,177,023 - +0.5 1,177,023 - -0.7 1,177,023 - -0.4 1,177,023 - -2.6 1,177,023 - +0.5 1,177,023 - -0.2 1,177,023 - -1.9 1,177,023 - -0.5 1,177,488 + ABIE41_RS05720 0.61 +0.1 1,177,489 - ABIE41_RS05720 0.61 -0.4 1,177,489 - ABIE41_RS05720 0.61 -1.2 1,177,494 + ABIE41_RS05720 0.62 -1.4 1,178,224 - ABIE41_RS05725 0.40 +0.5 1,178,280 + ABIE41_RS05725 0.47 -2.8 1,178,281 - ABIE41_RS05725 0.47 -1.7 1,178,281 - ABIE41_RS05725 0.47 -2.8 1,178,282 + ABIE41_RS05725 0.48 +0.4 1,178,282 + ABIE41_RS05725 0.48 +0.2 1,178,283 - ABIE41_RS05725 0.48 -2.2 1,178,317 - ABIE41_RS05725 0.52 -1.6 1,178,388 + ABIE41_RS05725 0.61 -1.3 1,178,388 + ABIE41_RS05725 0.61 -0.9 1,178,388 + ABIE41_RS05725 0.61 +0.2 1,178,388 + ABIE41_RS05725 0.61 -2.0 1,178,388 + ABIE41_RS05725 0.61 -1.0 1,178,388 + ABIE41_RS05725 0.61 -0.1 1,178,389 - ABIE41_RS05725 0.61 +1.7 1,178,389 - ABIE41_RS05725 0.61 -0.9 1,178,491 - ABIE41_RS05725 0.74 -0.3 1,178,491 - ABIE41_RS05725 0.74 +0.0 1,178,610 + ABIE41_RS05725 0.89 +0.9
Or see this region's nucleotide sequence