Experiment: R2A with 3-octanone 5.31033333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS03435 and ABIE41_RS03440 are separated by 12 nucleotides ABIE41_RS03440 and ABIE41_RS03445 overlap by 4 nucleotides ABIE41_RS03445 and ABIE41_RS03450 are separated by 229 nucleotides
ABIE41_RS03435: ABIE41_RS03435 - proline racemase family protein, at 713,933 to 714,952
_RS03435
ABIE41_RS03440: ABIE41_RS03440 - PfkB family carbohydrate kinase, at 714,965 to 715,894
_RS03440
ABIE41_RS03445: ABIE41_RS03445 - fumarylacetoacetate hydrolase family protein, at 715,891 to 717,069
_RS03445
ABIE41_RS03450: ABIE41_RS03450 - sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB, at 717,299 to 718,597
_RS03450
Position (kb) 715
716
717
718 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 714.930 kb on + strand at 714.939 kb on + strand at 714.940 kb on - strand at 714.940 kb on - strand at 715.160 kb on + strand, within ABIE41_RS03440 at 715.416 kb on - strand, within ABIE41_RS03440 at 715.422 kb on + strand, within ABIE41_RS03440 at 715.422 kb on + strand, within ABIE41_RS03440 at 715.422 kb on + strand, within ABIE41_RS03440 at 715.422 kb on + strand, within ABIE41_RS03440 at 715.422 kb on + strand, within ABIE41_RS03440 at 715.423 kb on - strand, within ABIE41_RS03440 at 715.423 kb on - strand, within ABIE41_RS03440 at 715.423 kb on - strand, within ABIE41_RS03440 at 715.746 kb on + strand, within ABIE41_RS03440 at 715.746 kb on + strand, within ABIE41_RS03440 at 715.747 kb on - strand, within ABIE41_RS03440 at 715.747 kb on - strand, within ABIE41_RS03440 at 715.818 kb on + strand at 715.818 kb on + strand at 716.027 kb on + strand, within ABIE41_RS03445 at 716.027 kb on + strand, within ABIE41_RS03445 at 716.028 kb on - strand, within ABIE41_RS03445 at 716.028 kb on - strand, within ABIE41_RS03445 at 716.056 kb on - strand, within ABIE41_RS03445 at 716.199 kb on + strand, within ABIE41_RS03445 at 716.199 kb on + strand, within ABIE41_RS03445 at 716.200 kb on - strand, within ABIE41_RS03445 at 716.200 kb on - strand, within ABIE41_RS03445 at 716.200 kb on - strand, within ABIE41_RS03445 at 716.397 kb on + strand, within ABIE41_RS03445 at 716.398 kb on - strand, within ABIE41_RS03445 at 716.398 kb on - strand, within ABIE41_RS03445 at 716.399 kb on - strand, within ABIE41_RS03445 at 716.552 kb on + strand, within ABIE41_RS03445 at 716.552 kb on + strand, within ABIE41_RS03445 at 716.552 kb on + strand, within ABIE41_RS03445 at 716.553 kb on - strand, within ABIE41_RS03445 at 716.553 kb on - strand, within ABIE41_RS03445 at 717.215 kb on + strand at 717.215 kb on + strand at 717.215 kb on + strand at 717.215 kb on + strand at 717.215 kb on + strand at 717.215 kb on + strand at 717.215 kb on + strand at 717.215 kb on + strand at 717.215 kb on + strand at 717.215 kb on + strand at 717.215 kb on + strand at 717.215 kb on + strand at 717.216 kb on - strand at 717.216 kb on - strand at 717.216 kb on - strand at 717.216 kb on - strand at 717.216 kb on - strand at 717.216 kb on - strand at 717.216 kb on - strand at 717.440 kb on + strand, within ABIE41_RS03450 at 717.440 kb on + strand, within ABIE41_RS03450 at 717.440 kb on + strand, within ABIE41_RS03450 at 717.440 kb on + strand, within ABIE41_RS03450 at 717.441 kb on - strand, within ABIE41_RS03450 at 717.441 kb on - strand, within ABIE41_RS03450 at 717.441 kb on - strand, within ABIE41_RS03450 at 717.441 kb on - strand, within ABIE41_RS03450 at 717.441 kb on - strand, within ABIE41_RS03450 at 717.441 kb on - strand, within ABIE41_RS03450 at 717.441 kb on - strand, within ABIE41_RS03450 at 717.441 kb on - strand, within ABIE41_RS03450 at 717.458 kb on + strand, within ABIE41_RS03450 at 717.458 kb on + strand, within ABIE41_RS03450 at 717.458 kb on + strand, within ABIE41_RS03450 at 717.459 kb on - strand, within ABIE41_RS03450 at 717.459 kb on - strand, within ABIE41_RS03450 at 717.459 kb on - strand, within ABIE41_RS03450 at 717.459 kb on - strand, within ABIE41_RS03450 at 717.460 kb on + strand, within ABIE41_RS03450 at 717.461 kb on - strand, within ABIE41_RS03450 at 717.461 kb on - strand, within ABIE41_RS03450 at 717.470 kb on + strand, within ABIE41_RS03450 at 717.638 kb on + strand, within ABIE41_RS03450 at 717.638 kb on + strand, within ABIE41_RS03450 at 717.638 kb on + strand, within ABIE41_RS03450 at 717.638 kb on + strand, within ABIE41_RS03450 at 717.639 kb on - strand, within ABIE41_RS03450 at 717.639 kb on - strand, within ABIE41_RS03450 at 717.639 kb on - strand, within ABIE41_RS03450 at 717.639 kb on - strand, within ABIE41_RS03450 at 717.639 kb on - strand, within ABIE41_RS03450 at 717.639 kb on - strand, within ABIE41_RS03450 at 717.659 kb on + strand, within ABIE41_RS03450 at 717.659 kb on + strand, within ABIE41_RS03450 at 717.659 kb on + strand, within ABIE41_RS03450 at 717.660 kb on - strand, within ABIE41_RS03450 at 717.660 kb on - strand, within ABIE41_RS03450 at 717.662 kb on + strand, within ABIE41_RS03450 at 717.662 kb on + strand, within ABIE41_RS03450 at 717.663 kb on - strand, within ABIE41_RS03450 at 717.663 kb on - strand, within ABIE41_RS03450 at 717.663 kb on - strand, within ABIE41_RS03450 at 717.725 kb on + strand, within ABIE41_RS03450 at 717.725 kb on + strand, within ABIE41_RS03450 at 717.725 kb on + strand, within ABIE41_RS03450 at 717.725 kb on + strand, within ABIE41_RS03450 at 717.726 kb on - strand, within ABIE41_RS03450 at 717.726 kb on - strand, within ABIE41_RS03450 at 717.726 kb on - strand, within ABIE41_RS03450 at 717.726 kb on - strand, within ABIE41_RS03450 at 717.726 kb on - strand, within ABIE41_RS03450 at 717.726 kb on - strand, within ABIE41_RS03450 at 718.022 kb on + strand, within ABIE41_RS03450
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.31033333333333 mM remove 714,930 + +0.2 714,939 + -0.8 714,940 - +1.7 714,940 - -0.1 715,160 + ABIE41_RS03440 0.21 -2.7 715,416 - ABIE41_RS03440 0.48 -1.2 715,422 + ABIE41_RS03440 0.49 +0.5 715,422 + ABIE41_RS03440 0.49 +1.2 715,422 + ABIE41_RS03440 0.49 +0.2 715,422 + ABIE41_RS03440 0.49 +0.0 715,422 + ABIE41_RS03440 0.49 -0.6 715,423 - ABIE41_RS03440 0.49 -0.7 715,423 - ABIE41_RS03440 0.49 +0.9 715,423 - ABIE41_RS03440 0.49 -0.3 715,746 + ABIE41_RS03440 0.84 +0.4 715,746 + ABIE41_RS03440 0.84 -0.7 715,747 - ABIE41_RS03440 0.84 +0.0 715,747 - ABIE41_RS03440 0.84 -0.5 715,818 + -0.2 715,818 + +0.3 716,027 + ABIE41_RS03445 0.12 +1.3 716,027 + ABIE41_RS03445 0.12 -0.9 716,028 - ABIE41_RS03445 0.12 +0.8 716,028 - ABIE41_RS03445 0.12 +2.2 716,056 - ABIE41_RS03445 0.14 -2.2 716,199 + ABIE41_RS03445 0.26 +0.9 716,199 + ABIE41_RS03445 0.26 +0.7 716,200 - ABIE41_RS03445 0.26 -0.8 716,200 - ABIE41_RS03445 0.26 -1.5 716,200 - ABIE41_RS03445 0.26 +0.4 716,397 + ABIE41_RS03445 0.43 +3.2 716,398 - ABIE41_RS03445 0.43 -0.4 716,398 - ABIE41_RS03445 0.43 +0.4 716,399 - ABIE41_RS03445 0.43 +0.3 716,552 + ABIE41_RS03445 0.56 -0.1 716,552 + ABIE41_RS03445 0.56 -1.8 716,552 + ABIE41_RS03445 0.56 -3.0 716,553 - ABIE41_RS03445 0.56 +0.6 716,553 - ABIE41_RS03445 0.56 +0.9 717,215 + -4.8 717,215 + +0.5 717,215 + -0.8 717,215 + -1.2 717,215 + -3.1 717,215 + +0.3 717,215 + +1.5 717,215 + -0.6 717,215 + -0.5 717,215 + +0.2 717,215 + -0.1 717,215 + -2.4 717,216 - -1.2 717,216 - +0.1 717,216 - -1.0 717,216 - -0.2 717,216 - -0.5 717,216 - -1.3 717,216 - +0.4 717,440 + ABIE41_RS03450 0.11 -2.3 717,440 + ABIE41_RS03450 0.11 +2.2 717,440 + ABIE41_RS03450 0.11 -2.2 717,440 + ABIE41_RS03450 0.11 +0.9 717,441 - ABIE41_RS03450 0.11 +0.7 717,441 - ABIE41_RS03450 0.11 -0.0 717,441 - ABIE41_RS03450 0.11 +3.2 717,441 - ABIE41_RS03450 0.11 +0.6 717,441 - ABIE41_RS03450 0.11 +0.0 717,441 - ABIE41_RS03450 0.11 +1.7 717,441 - ABIE41_RS03450 0.11 +0.6 717,441 - ABIE41_RS03450 0.11 -0.8 717,458 + ABIE41_RS03450 0.12 -0.3 717,458 + ABIE41_RS03450 0.12 +1.2 717,458 + ABIE41_RS03450 0.12 -0.4 717,459 - ABIE41_RS03450 0.12 -0.5 717,459 - ABIE41_RS03450 0.12 +0.9 717,459 - ABIE41_RS03450 0.12 -1.3 717,459 - ABIE41_RS03450 0.12 -2.6 717,460 + ABIE41_RS03450 0.12 -1.0 717,461 - ABIE41_RS03450 0.12 -0.1 717,461 - ABIE41_RS03450 0.12 -0.8 717,470 + ABIE41_RS03450 0.13 -0.2 717,638 + ABIE41_RS03450 0.26 -2.9 717,638 + ABIE41_RS03450 0.26 -1.8 717,638 + ABIE41_RS03450 0.26 +0.2 717,638 + ABIE41_RS03450 0.26 -0.8 717,639 - ABIE41_RS03450 0.26 +1.5 717,639 - ABIE41_RS03450 0.26 -1.7 717,639 - ABIE41_RS03450 0.26 +0.8 717,639 - ABIE41_RS03450 0.26 +0.8 717,639 - ABIE41_RS03450 0.26 -4.0 717,639 - ABIE41_RS03450 0.26 -0.6 717,659 + ABIE41_RS03450 0.28 -0.4 717,659 + ABIE41_RS03450 0.28 -0.2 717,659 + ABIE41_RS03450 0.28 +0.9 717,660 - ABIE41_RS03450 0.28 -2.0 717,660 - ABIE41_RS03450 0.28 +2.4 717,662 + ABIE41_RS03450 0.28 +0.2 717,662 + ABIE41_RS03450 0.28 -0.6 717,663 - ABIE41_RS03450 0.28 -0.4 717,663 - ABIE41_RS03450 0.28 -2.3 717,663 - ABIE41_RS03450 0.28 +1.2 717,725 + ABIE41_RS03450 0.33 +0.6 717,725 + ABIE41_RS03450 0.33 -0.5 717,725 + ABIE41_RS03450 0.33 +0.1 717,725 + ABIE41_RS03450 0.33 -1.1 717,726 - ABIE41_RS03450 0.33 +0.5 717,726 - ABIE41_RS03450 0.33 +0.2 717,726 - ABIE41_RS03450 0.33 -0.2 717,726 - ABIE41_RS03450 0.33 +0.4 717,726 - ABIE41_RS03450 0.33 +0.9 717,726 - ABIE41_RS03450 0.33 +0.1 718,022 + ABIE41_RS03450 0.56 -0.8
Or see this region's nucleotide sequence