Experiment: R2A with 3-octanone 5.31033333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS16795 and ABIE41_RS16800 are separated by 198 nucleotides ABIE41_RS16800 and ABIE41_RS16805 are separated by 4 nucleotides ABIE41_RS16805 and ABIE41_RS16810 are separated by 18 nucleotides
ABIE41_RS16795: ABIE41_RS16795 - tRNA dihydrouridine synthase DusB, at 3,463,199 to 3,464,183
_RS16795
ABIE41_RS16800: ABIE41_RS16800 - bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 3,464,382 to 3,465,593
_RS16800
ABIE41_RS16805: ABIE41_RS16805 - CinA family protein, at 3,465,598 to 3,466,089
_RS16805
ABIE41_RS16810: ABIE41_RS16810 - type II toxin-antitoxin system RatA family toxin, at 3,466,108 to 3,466,587
_RS16810
Position (kb)
3464
3465
3466 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3463.408 kb on - strand, within ABIE41_RS16795 at 3463.663 kb on + strand, within ABIE41_RS16795 at 3463.663 kb on + strand, within ABIE41_RS16795 at 3463.663 kb on + strand, within ABIE41_RS16795 at 3464.229 kb on + strand at 3464.230 kb on - strand at 3464.230 kb on - strand at 3464.274 kb on + strand at 3464.351 kb on + strand at 3464.352 kb on - strand at 3464.352 kb on - strand at 3465.763 kb on + strand, within ABIE41_RS16805 at 3465.763 kb on + strand, within ABIE41_RS16805 at 3465.764 kb on - strand, within ABIE41_RS16805 at 3466.149 kb on - strand at 3466.250 kb on + strand, within ABIE41_RS16810 at 3466.250 kb on + strand, within ABIE41_RS16810 at 3466.251 kb on - strand, within ABIE41_RS16810 at 3466.251 kb on - strand, within ABIE41_RS16810 at 3466.337 kb on + strand, within ABIE41_RS16810 at 3466.338 kb on - strand, within ABIE41_RS16810 at 3466.338 kb on - strand, within ABIE41_RS16810 at 3466.338 kb on - strand, within ABIE41_RS16810 at 3466.338 kb on - strand, within ABIE41_RS16810 at 3466.410 kb on - strand, within ABIE41_RS16810 at 3466.508 kb on + strand, within ABIE41_RS16810 at 3466.509 kb on - strand, within ABIE41_RS16810
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.31033333333333 mM remove 3,463,408 - ABIE41_RS16795 0.21 -0.7 3,463,663 + ABIE41_RS16795 0.47 -0.3 3,463,663 + ABIE41_RS16795 0.47 +0.1 3,463,663 + ABIE41_RS16795 0.47 +0.2 3,464,229 + +0.2 3,464,230 - +0.2 3,464,230 - +0.0 3,464,274 + +1.9 3,464,351 + +0.8 3,464,352 - -1.5 3,464,352 - -0.9 3,465,763 + ABIE41_RS16805 0.34 +2.2 3,465,763 + ABIE41_RS16805 0.34 -1.4 3,465,764 - ABIE41_RS16805 0.34 -1.4 3,466,149 - -0.3 3,466,250 + ABIE41_RS16810 0.30 -1.4 3,466,250 + ABIE41_RS16810 0.30 -1.5 3,466,251 - ABIE41_RS16810 0.30 -2.0 3,466,251 - ABIE41_RS16810 0.30 -0.9 3,466,337 + ABIE41_RS16810 0.48 -3.4 3,466,338 - ABIE41_RS16810 0.48 -2.8 3,466,338 - ABIE41_RS16810 0.48 -3.0 3,466,338 - ABIE41_RS16810 0.48 -2.6 3,466,338 - ABIE41_RS16810 0.48 -1.7 3,466,410 - ABIE41_RS16810 0.63 +0.8 3,466,508 + ABIE41_RS16810 0.83 +0.3 3,466,509 - ABIE41_RS16810 0.84 -2.0
Or see this region's nucleotide sequence