Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS04680 and ABIE41_RS04685 are separated by 69 nucleotides ABIE41_RS04685 and ABIE41_RS04690 are separated by 39 nucleotides ABIE41_RS04690 and ABIE41_RS04695 overlap by 4 nucleotides
ABIE41_RS04680: ABIE41_RS04680 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE, at 973,833 to 975,146
_RS04680
ABIE41_RS04685: ABIE41_RS04685 - tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG, at 975,216 to 977,099
_RS04685
ABIE41_RS04690: ABIE41_RS04690 - 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG, at 977,139 to 977,786
_RS04690
ABIE41_RS04695: ABIE41_RS04695 - ParA family protein, at 977,783 to 978,613
_RS04695
Position (kb) 975
976
977
978 Strain fitness (log2 ratio) -1
0
1 at 974.396 kb on + strand, within ABIE41_RS04680 at 974.397 kb on - strand, within ABIE41_RS04680 at 974.397 kb on - strand, within ABIE41_RS04680 at 974.528 kb on + strand, within ABIE41_RS04680 at 974.529 kb on - strand, within ABIE41_RS04680 at 974.529 kb on - strand, within ABIE41_RS04680 at 975.175 kb on + strand at 975.243 kb on + strand at 975.535 kb on - strand, within ABIE41_RS04685 at 976.740 kb on + strand, within ABIE41_RS04685 at 977.662 kb on + strand, within ABIE41_RS04690 at 977.662 kb on + strand, within ABIE41_RS04690
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 974,396 + ABIE41_RS04680 0.43 +0.1 974,397 - ABIE41_RS04680 0.43 +0.7 974,397 - ABIE41_RS04680 0.43 +0.9 974,528 + ABIE41_RS04680 0.53 +0.9 974,529 - ABIE41_RS04680 0.53 +0.2 974,529 - ABIE41_RS04680 0.53 +0.6 975,175 + +0.1 975,243 + -0.3 975,535 - ABIE41_RS04685 0.17 +1.3 976,740 + ABIE41_RS04685 0.81 +1.4 977,662 + ABIE41_RS04690 0.81 -0.5 977,662 + ABIE41_RS04690 0.81 -0.1
Or see this region's nucleotide sequence