Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS07605 and ABIE41_RS07610 are separated by 245 nucleotides ABIE41_RS07610 and ABIE41_RS07615 are separated by 99 nucleotides ABIE41_RS07615 and ABIE41_RS07620 are separated by 72 nucleotides
ABIE41_RS07605: ABIE41_RS07605 - hypothetical protein, at 1,557,492 to 1,557,812
_RS07605
ABIE41_RS07610: ABIE41_RS07610 - sigma-70 family RNA polymerase sigma factor, at 1,558,058 to 1,558,618
_RS07610
ABIE41_RS07615: ABIE41_RS07615 - hypothetical protein, at 1,558,718 to 1,559,395
_RS07615
ABIE41_RS07620: ABIE41_RS07620 - S8 family serine peptidase, at 1,559,468 to 1,560,907
_RS07620
Position (kb)
1558
1559
1560 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1557.956 kb on + strand at 1557.957 kb on - strand at 1558.007 kb on + strand at 1558.008 kb on - strand at 1558.008 kb on - strand at 1558.008 kb on - strand at 1558.008 kb on - strand at 1558.008 kb on - strand at 1558.008 kb on - strand at 1558.063 kb on + strand at 1558.064 kb on - strand at 1558.304 kb on + strand, within ABIE41_RS07610 at 1558.304 kb on + strand, within ABIE41_RS07610 at 1558.305 kb on - strand, within ABIE41_RS07610 at 1558.305 kb on - strand, within ABIE41_RS07610 at 1558.305 kb on - strand, within ABIE41_RS07610 at 1558.478 kb on + strand, within ABIE41_RS07610 at 1558.478 kb on + strand, within ABIE41_RS07610 at 1558.479 kb on - strand, within ABIE41_RS07610 at 1558.479 kb on - strand, within ABIE41_RS07610 at 1558.479 kb on - strand, within ABIE41_RS07610 at 1558.482 kb on - strand, within ABIE41_RS07610 at 1558.482 kb on - strand, within ABIE41_RS07610 at 1559.721 kb on + strand, within ABIE41_RS07620 at 1560.071 kb on + strand, within ABIE41_RS07620 at 1560.071 kb on + strand, within ABIE41_RS07620 at 1560.071 kb on + strand, within ABIE41_RS07620 at 1560.071 kb on + strand, within ABIE41_RS07620 at 1560.071 kb on + strand, within ABIE41_RS07620 at 1560.071 kb on + strand, within ABIE41_RS07620 at 1560.071 kb on + strand, within ABIE41_RS07620 at 1560.071 kb on + strand, within ABIE41_RS07620 at 1560.071 kb on + strand, within ABIE41_RS07620 at 1560.072 kb on - strand, within ABIE41_RS07620 at 1560.072 kb on - strand, within ABIE41_RS07620 at 1560.200 kb on + strand, within ABIE41_RS07620 at 1560.201 kb on - strand, within ABIE41_RS07620 at 1560.201 kb on - strand, within ABIE41_RS07620 at 1560.201 kb on - strand, within ABIE41_RS07620 at 1560.201 kb on - strand, within ABIE41_RS07620
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 1,557,956 + -0.1 1,557,957 - -0.3 1,558,007 + +0.4 1,558,008 - +1.1 1,558,008 - +0.3 1,558,008 - +0.8 1,558,008 - +0.6 1,558,008 - +2.4 1,558,008 - +0.0 1,558,063 + -0.7 1,558,064 - -0.0 1,558,304 + ABIE41_RS07610 0.44 -0.5 1,558,304 + ABIE41_RS07610 0.44 +1.4 1,558,305 - ABIE41_RS07610 0.44 -0.5 1,558,305 - ABIE41_RS07610 0.44 -0.5 1,558,305 - ABIE41_RS07610 0.44 -0.5 1,558,478 + ABIE41_RS07610 0.75 -0.3 1,558,478 + ABIE41_RS07610 0.75 -0.8 1,558,479 - ABIE41_RS07610 0.75 -0.3 1,558,479 - ABIE41_RS07610 0.75 -1.0 1,558,479 - ABIE41_RS07610 0.75 +0.0 1,558,482 - ABIE41_RS07610 0.76 +0.3 1,558,482 - ABIE41_RS07610 0.76 -2.0 1,559,721 + ABIE41_RS07620 0.18 -0.6 1,560,071 + ABIE41_RS07620 0.42 -0.4 1,560,071 + ABIE41_RS07620 0.42 -0.2 1,560,071 + ABIE41_RS07620 0.42 -0.4 1,560,071 + ABIE41_RS07620 0.42 +0.4 1,560,071 + ABIE41_RS07620 0.42 +0.1 1,560,071 + ABIE41_RS07620 0.42 -3.5 1,560,071 + ABIE41_RS07620 0.42 +0.1 1,560,071 + ABIE41_RS07620 0.42 -0.9 1,560,071 + ABIE41_RS07620 0.42 +0.1 1,560,072 - ABIE41_RS07620 0.42 +1.4 1,560,072 - ABIE41_RS07620 0.42 -1.0 1,560,200 + ABIE41_RS07620 0.51 +1.3 1,560,201 - ABIE41_RS07620 0.51 -0.5 1,560,201 - ABIE41_RS07620 0.51 -1.2 1,560,201 - ABIE41_RS07620 0.51 -0.1 1,560,201 - ABIE41_RS07620 0.51 +0.1
Or see this region's nucleotide sequence