Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS05485 and ABIE41_RS05490 are separated by 20 nucleotides ABIE41_RS05490 and ABIE41_RS05495 are separated by 131 nucleotides ABIE41_RS05495 and ABIE41_RS05500 are separated by 5 nucleotides
ABIE41_RS05485: ABIE41_RS05485 - flagellar biosynthesis repressor FlbT, at 1,132,808 to 1,133,272
_RS05485
ABIE41_RS05490: ABIE41_RS05490 - flagellar biosynthesis regulator FlaF, at 1,133,293 to 1,133,640
_RS05490
ABIE41_RS05495: ABIE41_RS05495 - flagellar hook-associated family protein, at 1,133,772 to 1,134,824
_RS05495
ABIE41_RS05500: ABIE41_RS05500 - flagellar hook-associated protein FlgK, at 1,134,830 to 1,136,311
_RS05500
Position (kb)
1133
1134
1135 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1132.803 kb on + strand at 1132.803 kb on + strand at 1132.873 kb on + strand, within ABIE41_RS05485 at 1132.873 kb on + strand, within ABIE41_RS05485 at 1132.874 kb on - strand, within ABIE41_RS05485 at 1132.874 kb on - strand, within ABIE41_RS05485 at 1132.923 kb on + strand, within ABIE41_RS05485 at 1132.923 kb on + strand, within ABIE41_RS05485 at 1133.112 kb on + strand, within ABIE41_RS05485 at 1133.112 kb on + strand, within ABIE41_RS05485 at 1133.112 kb on + strand, within ABIE41_RS05485 at 1133.112 kb on + strand, within ABIE41_RS05485 at 1133.112 kb on + strand, within ABIE41_RS05485 at 1133.113 kb on - strand, within ABIE41_RS05485 at 1133.113 kb on - strand, within ABIE41_RS05485 at 1133.113 kb on - strand, within ABIE41_RS05485 at 1133.113 kb on - strand, within ABIE41_RS05485 at 1133.238 kb on + strand at 1133.238 kb on + strand at 1133.238 kb on + strand at 1133.238 kb on + strand at 1133.238 kb on + strand at 1133.238 kb on + strand at 1133.239 kb on - strand at 1133.239 kb on - strand at 1133.239 kb on - strand at 1133.239 kb on - strand at 1133.239 kb on - strand at 1133.239 kb on - strand at 1133.239 kb on - strand at 1133.239 kb on - strand at 1133.239 kb on - strand at 1133.239 kb on - strand at 1133.239 kb on - strand at 1133.239 kb on - strand at 1133.600 kb on + strand, within ABIE41_RS05490 at 1133.600 kb on + strand, within ABIE41_RS05490 at 1133.601 kb on - strand, within ABIE41_RS05490 at 1133.601 kb on - strand, within ABIE41_RS05490 at 1133.601 kb on - strand, within ABIE41_RS05490 at 1133.601 kb on - strand, within ABIE41_RS05490 at 1133.601 kb on - strand, within ABIE41_RS05490 at 1133.616 kb on + strand at 1133.624 kb on + strand at 1133.624 kb on + strand at 1133.624 kb on + strand at 1133.636 kb on + strand at 1133.636 kb on + strand at 1133.636 kb on + strand at 1133.637 kb on - strand at 1133.637 kb on - strand at 1133.637 kb on - strand at 1133.637 kb on - strand at 1133.825 kb on - strand at 1133.825 kb on - strand at 1133.876 kb on - strand at 1134.088 kb on + strand, within ABIE41_RS05495 at 1134.089 kb on - strand, within ABIE41_RS05495 at 1134.100 kb on + strand, within ABIE41_RS05495 at 1134.101 kb on - strand, within ABIE41_RS05495 at 1134.101 kb on - strand, within ABIE41_RS05495 at 1134.101 kb on - strand, within ABIE41_RS05495 at 1134.101 kb on - strand, within ABIE41_RS05495 at 1134.326 kb on - strand, within ABIE41_RS05495 at 1134.382 kb on + strand, within ABIE41_RS05495 at 1134.414 kb on - strand, within ABIE41_RS05495 at 1134.649 kb on + strand, within ABIE41_RS05495 at 1134.649 kb on + strand, within ABIE41_RS05495 at 1134.650 kb on - strand, within ABIE41_RS05495 at 1134.650 kb on - strand, within ABIE41_RS05495 at 1134.650 kb on - strand, within ABIE41_RS05495 at 1134.706 kb on + strand, within ABIE41_RS05495 at 1134.712 kb on + strand, within ABIE41_RS05495 at 1134.799 kb on + strand at 1134.799 kb on + strand at 1134.800 kb on - strand at 1134.800 kb on - strand at 1134.800 kb on - strand at 1134.800 kb on - strand at 1134.800 kb on - strand at 1134.800 kb on - strand at 1134.800 kb on - strand at 1134.800 kb on - strand at 1134.800 kb on - strand at 1134.831 kb on + strand at 1134.831 kb on + strand at 1134.831 kb on + strand at 1134.832 kb on - strand at 1134.832 kb on - strand at 1134.832 kb on - strand at 1135.110 kb on + strand, within ABIE41_RS05500 at 1135.185 kb on + strand, within ABIE41_RS05500 at 1135.185 kb on + strand, within ABIE41_RS05500 at 1135.185 kb on + strand, within ABIE41_RS05500 at 1135.185 kb on + strand, within ABIE41_RS05500 at 1135.185 kb on + strand, within ABIE41_RS05500 at 1135.185 kb on + strand, within ABIE41_RS05500 at 1135.185 kb on + strand, within ABIE41_RS05500 at 1135.186 kb on - strand, within ABIE41_RS05500 at 1135.186 kb on - strand, within ABIE41_RS05500 at 1135.186 kb on - strand, within ABIE41_RS05500 at 1135.186 kb on - strand, within ABIE41_RS05500 at 1135.191 kb on + strand, within ABIE41_RS05500 at 1135.191 kb on + strand, within ABIE41_RS05500 at 1135.191 kb on + strand, within ABIE41_RS05500 at 1135.191 kb on + strand, within ABIE41_RS05500 at 1135.191 kb on + strand, within ABIE41_RS05500 at 1135.191 kb on + strand, within ABIE41_RS05500 at 1135.191 kb on + strand, within ABIE41_RS05500 at 1135.191 kb on + strand, within ABIE41_RS05500 at 1135.191 kb on + strand, within ABIE41_RS05500 at 1135.191 kb on + strand, within ABIE41_RS05500 at 1135.192 kb on - strand, within ABIE41_RS05500 at 1135.192 kb on - strand, within ABIE41_RS05500 at 1135.192 kb on - strand, within ABIE41_RS05500 at 1135.192 kb on - strand, within ABIE41_RS05500 at 1135.192 kb on - strand, within ABIE41_RS05500 at 1135.192 kb on - strand, within ABIE41_RS05500 at 1135.192 kb on - strand, within ABIE41_RS05500 at 1135.350 kb on + strand, within ABIE41_RS05500 at 1135.350 kb on + strand, within ABIE41_RS05500 at 1135.350 kb on + strand, within ABIE41_RS05500 at 1135.449 kb on + strand, within ABIE41_RS05500 at 1135.449 kb on + strand, within ABIE41_RS05500 at 1135.450 kb on - strand, within ABIE41_RS05500 at 1135.551 kb on + strand, within ABIE41_RS05500 at 1135.551 kb on + strand, within ABIE41_RS05500 at 1135.551 kb on + strand, within ABIE41_RS05500 at 1135.551 kb on + strand, within ABIE41_RS05500 at 1135.552 kb on - strand, within ABIE41_RS05500 at 1135.552 kb on - strand, within ABIE41_RS05500 at 1135.650 kb on + strand, within ABIE41_RS05500 at 1135.650 kb on + strand, within ABIE41_RS05500 at 1135.650 kb on + strand, within ABIE41_RS05500 at 1135.651 kb on - strand, within ABIE41_RS05500 at 1135.651 kb on - strand, within ABIE41_RS05500 at 1135.651 kb on - strand, within ABIE41_RS05500 at 1135.651 kb on - strand, within ABIE41_RS05500 at 1135.651 kb on - strand, within ABIE41_RS05500 at 1135.651 kb on - strand, within ABIE41_RS05500 at 1135.651 kb on - strand, within ABIE41_RS05500
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 1,132,803 + +0.3 1,132,803 + +0.4 1,132,873 + ABIE41_RS05485 0.14 +0.2 1,132,873 + ABIE41_RS05485 0.14 -0.9 1,132,874 - ABIE41_RS05485 0.14 +0.4 1,132,874 - ABIE41_RS05485 0.14 +0.9 1,132,923 + ABIE41_RS05485 0.25 +0.1 1,132,923 + ABIE41_RS05485 0.25 +0.6 1,133,112 + ABIE41_RS05485 0.65 +2.2 1,133,112 + ABIE41_RS05485 0.65 -3.3 1,133,112 + ABIE41_RS05485 0.65 +0.2 1,133,112 + ABIE41_RS05485 0.65 -0.8 1,133,112 + ABIE41_RS05485 0.65 -0.2 1,133,113 - ABIE41_RS05485 0.66 +0.8 1,133,113 - ABIE41_RS05485 0.66 +2.3 1,133,113 - ABIE41_RS05485 0.66 -2.1 1,133,113 - ABIE41_RS05485 0.66 +2.5 1,133,238 + +0.5 1,133,238 + +0.4 1,133,238 + -0.8 1,133,238 + +0.5 1,133,238 + -1.9 1,133,238 + -0.9 1,133,239 - +0.3 1,133,239 - +0.4 1,133,239 - +0.9 1,133,239 - +0.4 1,133,239 - -0.9 1,133,239 - -0.0 1,133,239 - +1.1 1,133,239 - +0.1 1,133,239 - +0.6 1,133,239 - +0.2 1,133,239 - +1.1 1,133,239 - +0.3 1,133,600 + ABIE41_RS05490 0.88 -0.9 1,133,600 + ABIE41_RS05490 0.88 +0.3 1,133,601 - ABIE41_RS05490 0.89 -0.5 1,133,601 - ABIE41_RS05490 0.89 -0.7 1,133,601 - ABIE41_RS05490 0.89 +0.6 1,133,601 - ABIE41_RS05490 0.89 +0.2 1,133,601 - ABIE41_RS05490 0.89 -0.3 1,133,616 + +0.5 1,133,624 + +0.5 1,133,624 + +0.7 1,133,624 + +0.0 1,133,636 + -0.2 1,133,636 + +0.0 1,133,636 + -0.2 1,133,637 - +0.3 1,133,637 - +0.4 1,133,637 - +0.2 1,133,637 - +0.4 1,133,825 - -0.0 1,133,825 - +0.3 1,133,876 - -0.0 1,134,088 + ABIE41_RS05495 0.30 +0.8 1,134,089 - ABIE41_RS05495 0.30 -0.9 1,134,100 + ABIE41_RS05495 0.31 +0.3 1,134,101 - ABIE41_RS05495 0.31 -0.2 1,134,101 - ABIE41_RS05495 0.31 -0.6 1,134,101 - ABIE41_RS05495 0.31 -1.9 1,134,101 - ABIE41_RS05495 0.31 -0.2 1,134,326 - ABIE41_RS05495 0.53 -0.2 1,134,382 + ABIE41_RS05495 0.58 +1.0 1,134,414 - ABIE41_RS05495 0.61 -0.8 1,134,649 + ABIE41_RS05495 0.83 +0.2 1,134,649 + ABIE41_RS05495 0.83 -0.7 1,134,650 - ABIE41_RS05495 0.83 -0.8 1,134,650 - ABIE41_RS05495 0.83 -0.1 1,134,650 - ABIE41_RS05495 0.83 +0.4 1,134,706 + ABIE41_RS05495 0.89 -1.1 1,134,712 + ABIE41_RS05495 0.89 -0.1 1,134,799 + +0.1 1,134,799 + -1.5 1,134,800 - +1.3 1,134,800 - -0.3 1,134,800 - +0.4 1,134,800 - +0.1 1,134,800 - +0.5 1,134,800 - -0.5 1,134,800 - -0.4 1,134,800 - +0.2 1,134,800 - +0.1 1,134,831 + -0.1 1,134,831 + -0.3 1,134,831 + -0.5 1,134,832 - +0.6 1,134,832 - +0.4 1,134,832 - +1.1 1,135,110 + ABIE41_RS05500 0.19 -0.8 1,135,185 + ABIE41_RS05500 0.24 +0.1 1,135,185 + ABIE41_RS05500 0.24 +0.9 1,135,185 + ABIE41_RS05500 0.24 +0.1 1,135,185 + ABIE41_RS05500 0.24 +0.3 1,135,185 + ABIE41_RS05500 0.24 -0.1 1,135,185 + ABIE41_RS05500 0.24 +1.8 1,135,185 + ABIE41_RS05500 0.24 -1.5 1,135,186 - ABIE41_RS05500 0.24 -0.4 1,135,186 - ABIE41_RS05500 0.24 -0.6 1,135,186 - ABIE41_RS05500 0.24 +1.1 1,135,186 - ABIE41_RS05500 0.24 -0.2 1,135,191 + ABIE41_RS05500 0.24 -0.1 1,135,191 + ABIE41_RS05500 0.24 -0.0 1,135,191 + ABIE41_RS05500 0.24 +0.5 1,135,191 + ABIE41_RS05500 0.24 +1.5 1,135,191 + ABIE41_RS05500 0.24 +0.8 1,135,191 + ABIE41_RS05500 0.24 +1.7 1,135,191 + ABIE41_RS05500 0.24 +1.2 1,135,191 + ABIE41_RS05500 0.24 +0.5 1,135,191 + ABIE41_RS05500 0.24 +0.1 1,135,191 + ABIE41_RS05500 0.24 +2.0 1,135,192 - ABIE41_RS05500 0.24 +1.0 1,135,192 - ABIE41_RS05500 0.24 +0.2 1,135,192 - ABIE41_RS05500 0.24 +0.5 1,135,192 - ABIE41_RS05500 0.24 -0.2 1,135,192 - ABIE41_RS05500 0.24 -2.5 1,135,192 - ABIE41_RS05500 0.24 +0.8 1,135,192 - ABIE41_RS05500 0.24 -0.8 1,135,350 + ABIE41_RS05500 0.35 +0.7 1,135,350 + ABIE41_RS05500 0.35 +0.3 1,135,350 + ABIE41_RS05500 0.35 -0.1 1,135,449 + ABIE41_RS05500 0.42 +0.3 1,135,449 + ABIE41_RS05500 0.42 +0.7 1,135,450 - ABIE41_RS05500 0.42 -0.8 1,135,551 + ABIE41_RS05500 0.49 -0.5 1,135,551 + ABIE41_RS05500 0.49 +0.6 1,135,551 + ABIE41_RS05500 0.49 -0.4 1,135,551 + ABIE41_RS05500 0.49 +0.9 1,135,552 - ABIE41_RS05500 0.49 -0.1 1,135,552 - ABIE41_RS05500 0.49 -1.0 1,135,650 + ABIE41_RS05500 0.55 -0.2 1,135,650 + ABIE41_RS05500 0.55 -1.0 1,135,650 + ABIE41_RS05500 0.55 +3.4 1,135,651 - ABIE41_RS05500 0.55 +0.1 1,135,651 - ABIE41_RS05500 0.55 -0.1 1,135,651 - ABIE41_RS05500 0.55 +1.0 1,135,651 - ABIE41_RS05500 0.55 +2.8 1,135,651 - ABIE41_RS05500 0.55 +0.5 1,135,651 - ABIE41_RS05500 0.55 -0.4 1,135,651 - ABIE41_RS05500 0.55 -0.5
Or see this region's nucleotide sequence