Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS20825 and ABIE41_RS20830 are separated by 161 nucleotides ABIE41_RS20830 and ABIE41_RS20835 are separated by 63 nucleotides
ABIE41_RS20825: ABIE41_RS20825 - phosphatidylcholine/phosphatidylserine synthase, at 4,281,093 to 4,281,941
_RS20825
ABIE41_RS20830: ABIE41_RS20830 - phosphatidylserine decarboxylase, at 4,282,103 to 4,282,915
_RS20830
ABIE41_RS20835: ABIE41_RS20835 - cytochrome P450, at 4,282,979 to 4,284,343
_RS20835
Position (kb) 4282
4283 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 4281.931 kb on - strand at 4281.931 kb on - strand at 4282.963 kb on + strand at 4283.103 kb on - strand at 4283.103 kb on - strand at 4283.150 kb on + strand, within ABIE41_RS20835 at 4283.150 kb on + strand, within ABIE41_RS20835 at 4283.150 kb on + strand, within ABIE41_RS20835 at 4283.151 kb on - strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.535 kb on - strand, within ABIE41_RS20835 at 4283.546 kb on + strand, within ABIE41_RS20835 at 4283.546 kb on + strand, within ABIE41_RS20835 at 4283.547 kb on - strand, within ABIE41_RS20835 at 4283.547 kb on - strand, within ABIE41_RS20835 at 4283.615 kb on + strand, within ABIE41_RS20835 at 4283.616 kb on - strand, within ABIE41_RS20835 at 4283.616 kb on - strand, within ABIE41_RS20835 at 4283.905 kb on + strand, within ABIE41_RS20835
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 4,281,931 - +0.2 4,281,931 - -0.1 4,282,963 + -0.7 4,283,103 - -0.9 4,283,103 - -0.6 4,283,150 + ABIE41_RS20835 0.13 +0.7 4,283,150 + ABIE41_RS20835 0.13 +0.1 4,283,150 + ABIE41_RS20835 0.13 +4.5 4,283,151 - ABIE41_RS20835 0.13 -0.4 4,283,534 + ABIE41_RS20835 0.41 +1.5 4,283,534 + ABIE41_RS20835 0.41 +1.1 4,283,534 + ABIE41_RS20835 0.41 -0.6 4,283,534 + ABIE41_RS20835 0.41 -0.4 4,283,534 + ABIE41_RS20835 0.41 -1.4 4,283,534 + ABIE41_RS20835 0.41 +3.1 4,283,534 + ABIE41_RS20835 0.41 -0.3 4,283,535 - ABIE41_RS20835 0.41 -0.0 4,283,546 + ABIE41_RS20835 0.42 -2.0 4,283,546 + ABIE41_RS20835 0.42 -0.7 4,283,547 - ABIE41_RS20835 0.42 -0.3 4,283,547 - ABIE41_RS20835 0.42 +0.3 4,283,615 + ABIE41_RS20835 0.47 -0.0 4,283,616 - ABIE41_RS20835 0.47 +0.1 4,283,616 - ABIE41_RS20835 0.47 -1.0 4,283,905 + ABIE41_RS20835 0.68 +0.1
Or see this region's nucleotide sequence