Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS18090 and ABIE41_RS18095 are separated by 117 nucleotides ABIE41_RS18095 and ABIE41_RS18100 are separated by 5 nucleotides ABIE41_RS18100 and ABIE41_RS18105 are separated by 158 nucleotides ABIE41_RS18105 and ABIE41_RS18110 are separated by 45 nucleotides
ABIE41_RS18090: ABIE41_RS18090 - phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating), at 3,726,572 to 3,727,558
_RS18090
ABIE41_RS18095: ABIE41_RS18095 - hypothetical protein, at 3,727,676 to 3,727,978
_RS18095
ABIE41_RS18100: ABIE41_RS18100 - 1-aminocyclopropane-1-carboxylate deaminase, at 3,727,984 to 3,728,994
_RS18100
ABIE41_RS18105: ABIE41_RS18105 - Lrp/AsnC family transcriptional regulator, at 3,729,153 to 3,729,617
_RS18105
ABIE41_RS18110: ABIE41_RS18110 - polyphosphate kinase 2, at 3,729,663 to 3,730,697
_RS18110
Position (kb)
3727
3728
3729 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4
5 at 3727.032 kb on + strand, within ABIE41_RS18090 at 3727.032 kb on + strand, within ABIE41_RS18090 at 3727.033 kb on - strand, within ABIE41_RS18090 at 3727.215 kb on + strand, within ABIE41_RS18090 at 3727.216 kb on - strand, within ABIE41_RS18090 at 3727.216 kb on - strand, within ABIE41_RS18090 at 3727.216 kb on - strand, within ABIE41_RS18090 at 3727.216 kb on - strand, within ABIE41_RS18090 at 3728.003 kb on + strand at 3728.003 kb on + strand at 3728.003 kb on + strand at 3728.003 kb on + strand at 3728.003 kb on + strand at 3728.003 kb on + strand at 3728.004 kb on - strand at 3728.004 kb on - strand at 3728.004 kb on - strand at 3728.004 kb on - strand at 3728.004 kb on - strand at 3728.004 kb on - strand at 3728.004 kb on - strand at 3728.009 kb on + strand at 3728.009 kb on + strand at 3728.045 kb on + strand at 3728.046 kb on - strand at 3728.046 kb on - strand at 3728.046 kb on - strand at 3728.046 kb on - strand at 3728.121 kb on - strand, within ABIE41_RS18100 at 3728.213 kb on + strand, within ABIE41_RS18100 at 3728.213 kb on + strand, within ABIE41_RS18100 at 3728.214 kb on - strand, within ABIE41_RS18100 at 3728.421 kb on + strand, within ABIE41_RS18100 at 3728.421 kb on + strand, within ABIE41_RS18100 at 3728.422 kb on - strand, within ABIE41_RS18100 at 3728.422 kb on - strand, within ABIE41_RS18100 at 3728.422 kb on - strand, within ABIE41_RS18100 at 3728.422 kb on - strand, within ABIE41_RS18100 at 3728.424 kb on - strand, within ABIE41_RS18100 at 3728.483 kb on + strand, within ABIE41_RS18100 at 3728.483 kb on + strand, within ABIE41_RS18100 at 3728.484 kb on - strand, within ABIE41_RS18100 at 3728.669 kb on + strand, within ABIE41_RS18100 at 3728.670 kb on - strand, within ABIE41_RS18100 at 3728.828 kb on + strand, within ABIE41_RS18100 at 3728.828 kb on + strand, within ABIE41_RS18100 at 3728.828 kb on + strand, within ABIE41_RS18100 at 3728.828 kb on + strand, within ABIE41_RS18100 at 3728.829 kb on - strand, within ABIE41_RS18100 at 3728.829 kb on - strand, within ABIE41_RS18100 at 3728.829 kb on - strand, within ABIE41_RS18100 at 3728.829 kb on - strand, within ABIE41_RS18100 at 3728.829 kb on - strand, within ABIE41_RS18100 at 3728.832 kb on - strand, within ABIE41_RS18100 at 3728.973 kb on - strand at 3729.128 kb on - strand at 3729.300 kb on + strand, within ABIE41_RS18105 at 3729.300 kb on + strand, within ABIE41_RS18105 at 3729.301 kb on - strand, within ABIE41_RS18105 at 3729.301 kb on - strand, within ABIE41_RS18105 at 3729.301 kb on - strand, within ABIE41_RS18105 at 3729.475 kb on - strand, within ABIE41_RS18105 at 3729.475 kb on - strand, within ABIE41_RS18105 at 3729.769 kb on + strand, within ABIE41_RS18110 at 3729.790 kb on + strand, within ABIE41_RS18110 at 3729.791 kb on - strand, within ABIE41_RS18110 at 3729.895 kb on + strand, within ABIE41_RS18110 at 3729.898 kb on + strand, within ABIE41_RS18110 at 3729.898 kb on + strand, within ABIE41_RS18110 at 3729.899 kb on - strand, within ABIE41_RS18110 at 3729.899 kb on - strand, within ABIE41_RS18110 at 3729.899 kb on - strand, within ABIE41_RS18110 at 3729.899 kb on - strand, within ABIE41_RS18110 at 3729.899 kb on - strand, within ABIE41_RS18110
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 3,727,032 + ABIE41_RS18090 0.47 +0.6 3,727,032 + ABIE41_RS18090 0.47 -0.9 3,727,033 - ABIE41_RS18090 0.47 -0.8 3,727,215 + ABIE41_RS18090 0.65 +2.7 3,727,216 - ABIE41_RS18090 0.65 +0.4 3,727,216 - ABIE41_RS18090 0.65 -2.8 3,727,216 - ABIE41_RS18090 0.65 -0.3 3,727,216 - ABIE41_RS18090 0.65 +5.1 3,728,003 + -0.5 3,728,003 + -0.9 3,728,003 + +0.0 3,728,003 + -0.5 3,728,003 + +0.9 3,728,003 + -2.1 3,728,004 - +0.9 3,728,004 - +0.7 3,728,004 - +0.5 3,728,004 - +1.4 3,728,004 - +0.1 3,728,004 - -0.7 3,728,004 - +0.0 3,728,009 + +2.7 3,728,009 + +1.1 3,728,045 + +1.4 3,728,046 - +0.3 3,728,046 - +0.3 3,728,046 - -3.1 3,728,046 - +1.6 3,728,121 - ABIE41_RS18100 0.14 +0.5 3,728,213 + ABIE41_RS18100 0.23 +0.2 3,728,213 + ABIE41_RS18100 0.23 +1.7 3,728,214 - ABIE41_RS18100 0.23 +2.3 3,728,421 + ABIE41_RS18100 0.43 +2.1 3,728,421 + ABIE41_RS18100 0.43 +0.5 3,728,422 - ABIE41_RS18100 0.43 -4.1 3,728,422 - ABIE41_RS18100 0.43 +1.6 3,728,422 - ABIE41_RS18100 0.43 +1.1 3,728,422 - ABIE41_RS18100 0.43 -0.3 3,728,424 - ABIE41_RS18100 0.44 +0.5 3,728,483 + ABIE41_RS18100 0.49 -0.9 3,728,483 + ABIE41_RS18100 0.49 -0.1 3,728,484 - ABIE41_RS18100 0.49 -0.8 3,728,669 + ABIE41_RS18100 0.68 +0.1 3,728,670 - ABIE41_RS18100 0.68 -1.3 3,728,828 + ABIE41_RS18100 0.83 -0.7 3,728,828 + ABIE41_RS18100 0.83 +0.8 3,728,828 + ABIE41_RS18100 0.83 -1.6 3,728,828 + ABIE41_RS18100 0.83 +0.4 3,728,829 - ABIE41_RS18100 0.84 -1.9 3,728,829 - ABIE41_RS18100 0.84 -0.2 3,728,829 - ABIE41_RS18100 0.84 +1.2 3,728,829 - ABIE41_RS18100 0.84 -0.3 3,728,829 - ABIE41_RS18100 0.84 -1.6 3,728,832 - ABIE41_RS18100 0.84 +2.3 3,728,973 - -0.3 3,729,128 - +0.5 3,729,300 + ABIE41_RS18105 0.32 -1.2 3,729,300 + ABIE41_RS18105 0.32 +0.1 3,729,301 - ABIE41_RS18105 0.32 -1.0 3,729,301 - ABIE41_RS18105 0.32 +1.9 3,729,301 - ABIE41_RS18105 0.32 -1.5 3,729,475 - ABIE41_RS18105 0.69 +0.0 3,729,475 - ABIE41_RS18105 0.69 -0.9 3,729,769 + ABIE41_RS18110 0.10 +1.6 3,729,790 + ABIE41_RS18110 0.12 -0.2 3,729,791 - ABIE41_RS18110 0.12 +0.5 3,729,895 + ABIE41_RS18110 0.22 -1.3 3,729,898 + ABIE41_RS18110 0.23 -0.7 3,729,898 + ABIE41_RS18110 0.23 -0.8 3,729,899 - ABIE41_RS18110 0.23 -0.1 3,729,899 - ABIE41_RS18110 0.23 -0.8 3,729,899 - ABIE41_RS18110 0.23 -2.5 3,729,899 - ABIE41_RS18110 0.23 -1.6 3,729,899 - ABIE41_RS18110 0.23 +0.8
Or see this region's nucleotide sequence