Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS12890 and ABIE41_RS12895 are separated by 122 nucleotides ABIE41_RS12895 and ABIE41_RS12900 overlap by 4 nucleotides ABIE41_RS12900 and ABIE41_RS12905 overlap by 4 nucleotides ABIE41_RS12905 and ABIE41_RS12910 are separated by 39 nucleotides ABIE41_RS12910 and ABIE41_RS12915 are separated by 112 nucleotides
ABIE41_RS12890: ABIE41_RS12890 - acetolactate synthase 3 large subunit, at 2,639,327 to 2,641,090
_RS12890
ABIE41_RS12895: ABIE41_RS12895 - aspartate/glutamate racemase family protein, at 2,641,213 to 2,641,920
_RS12895
ABIE41_RS12900: ABIE41_RS12900 - glutathione S-transferase family protein, at 2,641,917 to 2,642,630
_RS12900
ABIE41_RS12905: ABIE41_RS12905 - acetolactate synthase small subunit, at 2,642,627 to 2,643,166
_RS12905
ABIE41_RS12910: ABIE41_RS12910 - hypothetical protein, at 2,643,206 to 2,643,421
_RS12910
ABIE41_RS12915: ABIE41_RS12915 - TetR/AcrR family transcriptional regulator C-terminal domain-containing protein, at 2,643,534 to 2,644,202
_RS12915
Position (kb)
2641
2642
2643 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2641.264 kb on + strand at 2641.264 kb on + strand at 2641.264 kb on + strand at 2641.267 kb on + strand at 2641.612 kb on + strand, within ABIE41_RS12895 at 2641.612 kb on + strand, within ABIE41_RS12895 at 2641.693 kb on + strand, within ABIE41_RS12895 at 2641.742 kb on - strand, within ABIE41_RS12895 at 2641.944 kb on + strand at 2641.944 kb on + strand at 2641.944 kb on + strand at 2641.944 kb on + strand at 2641.945 kb on - strand at 2641.945 kb on - strand at 2641.945 kb on - strand at 2642.136 kb on + strand, within ABIE41_RS12900 at 2642.142 kb on + strand, within ABIE41_RS12900 at 2642.143 kb on - strand, within ABIE41_RS12900 at 2642.143 kb on - strand, within ABIE41_RS12900 at 2642.481 kb on + strand, within ABIE41_RS12900 at 2642.481 kb on + strand, within ABIE41_RS12900 at 2642.756 kb on + strand, within ABIE41_RS12905 at 2642.757 kb on - strand, within ABIE41_RS12905 at 2643.164 kb on + strand at 2643.164 kb on + strand at 2643.164 kb on + strand at 2643.164 kb on + strand at 2643.164 kb on + strand at 2643.164 kb on + strand at 2643.164 kb on + strand at 2643.165 kb on - strand at 2643.165 kb on - strand at 2643.165 kb on - strand at 2643.165 kb on - strand at 2643.165 kb on - strand at 2643.481 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 2,641,264 + -0.9 2,641,264 + +0.7 2,641,264 + -1.2 2,641,267 + +0.5 2,641,612 + ABIE41_RS12895 0.56 -1.5 2,641,612 + ABIE41_RS12895 0.56 +0.1 2,641,693 + ABIE41_RS12895 0.68 +3.8 2,641,742 - ABIE41_RS12895 0.75 +1.1 2,641,944 + +0.0 2,641,944 + -0.3 2,641,944 + +0.3 2,641,944 + +0.6 2,641,945 - -0.0 2,641,945 - +0.5 2,641,945 - +0.5 2,642,136 + ABIE41_RS12900 0.31 -1.3 2,642,142 + ABIE41_RS12900 0.32 -1.0 2,642,143 - ABIE41_RS12900 0.32 -0.8 2,642,143 - ABIE41_RS12900 0.32 -0.8 2,642,481 + ABIE41_RS12900 0.79 -1.0 2,642,481 + ABIE41_RS12900 0.79 -1.6 2,642,756 + ABIE41_RS12905 0.24 +0.6 2,642,757 - ABIE41_RS12905 0.24 +0.6 2,643,164 + +0.8 2,643,164 + -0.5 2,643,164 + +0.7 2,643,164 + +0.2 2,643,164 + +0.7 2,643,164 + -2.1 2,643,164 + -0.1 2,643,165 - +0.3 2,643,165 - -1.0 2,643,165 - -0.0 2,643,165 - -0.8 2,643,165 - +1.0 2,643,481 + -0.8
Or see this region's nucleotide sequence