Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS11955 and ABIE41_RS11960 are separated by 3 nucleotides ABIE41_RS11960 and ABIE41_RS11965 are separated by 79 nucleotides ABIE41_RS11965 and ABIE41_RS11970 overlap by 4 nucleotides
ABIE41_RS11955: ABIE41_RS11955 - LysR substrate-binding domain-containing protein, at 2,443,032 to 2,443,967
_RS11955
ABIE41_RS11960: ABIE41_RS11960 - group III truncated hemoglobin, at 2,443,971 to 2,444,399
_RS11960
ABIE41_RS11965: ABIE41_RS11965 - ABC-F family ATP-binding cassette domain-containing protein, at 2,444,479 to 2,446,101
_RS11965
ABIE41_RS11970: ABIE41_RS11970 - prephenate dehydrogenase, at 2,446,098 to 2,446,967
_RS11970
Position (kb)
2444
2445
2446
2447 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2443.704 kb on + strand, within ABIE41_RS11955 at 2443.704 kb on + strand, within ABIE41_RS11955 at 2443.705 kb on - strand, within ABIE41_RS11955 at 2443.705 kb on - strand, within ABIE41_RS11955 at 2443.705 kb on - strand, within ABIE41_RS11955 at 2443.705 kb on - strand, within ABIE41_RS11955 at 2443.895 kb on + strand at 2443.895 kb on + strand at 2443.896 kb on - strand at 2444.191 kb on + strand, within ABIE41_RS11960 at 2444.192 kb on - strand, within ABIE41_RS11960 at 2444.314 kb on + strand, within ABIE41_RS11960 at 2444.314 kb on + strand, within ABIE41_RS11960 at 2444.315 kb on - strand, within ABIE41_RS11960 at 2444.434 kb on + strand at 2444.434 kb on + strand at 2444.435 kb on - strand at 2444.523 kb on - strand at 2444.523 kb on - strand at 2444.532 kb on - strand at 2444.543 kb on + strand at 2444.543 kb on + strand at 2444.544 kb on - strand at 2444.544 kb on - strand at 2444.544 kb on - strand at 2444.640 kb on - strand at 2445.083 kb on + strand, within ABIE41_RS11965 at 2445.084 kb on - strand, within ABIE41_RS11965 at 2445.084 kb on - strand, within ABIE41_RS11965 at 2445.101 kb on + strand, within ABIE41_RS11965 at 2445.214 kb on - strand, within ABIE41_RS11965 at 2445.365 kb on + strand, within ABIE41_RS11965 at 2445.365 kb on + strand, within ABIE41_RS11965 at 2445.375 kb on - strand, within ABIE41_RS11965 at 2445.386 kb on + strand, within ABIE41_RS11965 at 2445.387 kb on - strand, within ABIE41_RS11965 at 2445.699 kb on - strand, within ABIE41_RS11965 at 2445.900 kb on - strand, within ABIE41_RS11965 at 2446.077 kb on + strand at 2446.127 kb on + strand at 2446.127 kb on + strand at 2446.128 kb on - strand at 2446.128 kb on - strand at 2446.128 kb on - strand at 2446.128 kb on - strand at 2446.140 kb on + strand at 2447.043 kb on + strand at 2447.044 kb on - strand at 2447.044 kb on - strand at 2447.044 kb on - strand at 2447.076 kb on + strand at 2447.076 kb on + strand at 2447.077 kb on - strand at 2447.079 kb on + strand at 2447.079 kb on + strand at 2447.079 kb on + strand at 2447.079 kb on + strand at 2447.080 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 2,443,704 + ABIE41_RS11955 0.72 +0.2 2,443,704 + ABIE41_RS11955 0.72 +1.2 2,443,705 - ABIE41_RS11955 0.72 -0.4 2,443,705 - ABIE41_RS11955 0.72 -3.8 2,443,705 - ABIE41_RS11955 0.72 +0.1 2,443,705 - ABIE41_RS11955 0.72 +0.5 2,443,895 + +0.4 2,443,895 + -0.4 2,443,896 - -2.1 2,444,191 + ABIE41_RS11960 0.51 +1.0 2,444,192 - ABIE41_RS11960 0.52 -0.8 2,444,314 + ABIE41_RS11960 0.80 -0.2 2,444,314 + ABIE41_RS11960 0.80 -0.4 2,444,315 - ABIE41_RS11960 0.80 +1.0 2,444,434 + -0.3 2,444,434 + -0.4 2,444,435 - -0.1 2,444,523 - -1.2 2,444,523 - +0.0 2,444,532 - -0.4 2,444,543 + -0.3 2,444,543 + +0.7 2,444,544 - +1.4 2,444,544 - -0.8 2,444,544 - -2.7 2,444,640 - +0.3 2,445,083 + ABIE41_RS11965 0.37 -1.4 2,445,084 - ABIE41_RS11965 0.37 +0.1 2,445,084 - ABIE41_RS11965 0.37 -0.3 2,445,101 + ABIE41_RS11965 0.38 -1.7 2,445,214 - ABIE41_RS11965 0.45 -0.4 2,445,365 + ABIE41_RS11965 0.55 +0.7 2,445,365 + ABIE41_RS11965 0.55 -0.3 2,445,375 - ABIE41_RS11965 0.55 +1.1 2,445,386 + ABIE41_RS11965 0.56 -2.5 2,445,387 - ABIE41_RS11965 0.56 +0.5 2,445,699 - ABIE41_RS11965 0.75 +1.0 2,445,900 - ABIE41_RS11965 0.88 -0.1 2,446,077 + +0.1 2,446,127 + -0.3 2,446,127 + -0.4 2,446,128 - -1.2 2,446,128 - -0.2 2,446,128 - -0.1 2,446,128 - +1.3 2,446,140 + -0.7 2,447,043 + +0.4 2,447,044 - +0.7 2,447,044 - +3.3 2,447,044 - -2.4 2,447,076 + -1.1 2,447,076 + +0.5 2,447,077 - -0.5 2,447,079 + +0.7 2,447,079 + -0.4 2,447,079 + -0.2 2,447,079 + -0.9 2,447,080 - +0.8
Or see this region's nucleotide sequence