Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS09260 and ABIE41_RS09265 are separated by 41 nucleotides ABIE41_RS09265 and ABIE41_RS09270 are separated by 70 nucleotides ABIE41_RS09270 and ABIE41_RS09275 are separated by 6 nucleotides ABIE41_RS09275 and ABIE41_RS09280 overlap by 8 nucleotides
ABIE41_RS09260: ABIE41_RS09260 - alpha/beta fold hydrolase, at 1,920,012 to 1,920,857
_RS09260
ABIE41_RS09265: ABIE41_RS09265 - GNAT family N-acetyltransferase, at 1,920,899 to 1,921,378
_RS09265
ABIE41_RS09270: ABIE41_RS09270 - NAD(P)(+) transhydrogenase (Re/Si-specific) subunit beta, at 1,921,449 to 1,922,855
_RS09270
ABIE41_RS09275: ABIE41_RS09275 - NAD(P) transhydrogenase subunit alpha, at 1,922,862 to 1,923,299
_RS09275
ABIE41_RS09280: ABIE41_RS09280 - Re/Si-specific NAD(P)(+) transhydrogenase subunit alpha, at 1,923,292 to 1,924,488
_RS09280
Position (kb)
1921
1922
1923 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1920.464 kb on - strand, within ABIE41_RS09260 at 1920.464 kb on - strand, within ABIE41_RS09260 at 1920.523 kb on + strand, within ABIE41_RS09260 at 1920.524 kb on - strand, within ABIE41_RS09260 at 1920.532 kb on + strand, within ABIE41_RS09260 at 1920.533 kb on - strand, within ABIE41_RS09260 at 1920.628 kb on + strand, within ABIE41_RS09260 at 1920.815 kb on - strand at 1921.134 kb on + strand, within ABIE41_RS09265 at 1921.134 kb on + strand, within ABIE41_RS09265 at 1921.135 kb on - strand, within ABIE41_RS09265 at 1921.135 kb on - strand, within ABIE41_RS09265 at 1921.135 kb on - strand, within ABIE41_RS09265 at 1921.135 kb on - strand, within ABIE41_RS09265 at 1921.135 kb on - strand, within ABIE41_RS09265 at 1921.135 kb on - strand, within ABIE41_RS09265 at 1921.253 kb on + strand, within ABIE41_RS09265 at 1921.624 kb on + strand, within ABIE41_RS09270 at 1921.625 kb on - strand, within ABIE41_RS09270 at 1921.819 kb on + strand, within ABIE41_RS09270 at 1921.819 kb on + strand, within ABIE41_RS09270 at 1921.823 kb on + strand, within ABIE41_RS09270 at 1921.824 kb on - strand, within ABIE41_RS09270 at 1921.942 kb on + strand, within ABIE41_RS09270 at 1921.942 kb on + strand, within ABIE41_RS09270 at 1921.943 kb on - strand, within ABIE41_RS09270 at 1922.080 kb on + strand, within ABIE41_RS09270 at 1922.080 kb on + strand, within ABIE41_RS09270 at 1922.080 kb on + strand, within ABIE41_RS09270 at 1922.080 kb on + strand, within ABIE41_RS09270 at 1922.080 kb on + strand, within ABIE41_RS09270 at 1922.080 kb on + strand, within ABIE41_RS09270 at 1922.081 kb on - strand, within ABIE41_RS09270 at 1922.081 kb on - strand, within ABIE41_RS09270 at 1922.180 kb on - strand, within ABIE41_RS09270 at 1922.180 kb on - strand, within ABIE41_RS09270 at 1922.528 kb on - strand, within ABIE41_RS09270 at 1922.749 kb on + strand at 1922.749 kb on + strand at 1922.827 kb on + strand at 1922.827 kb on + strand at 1922.827 kb on + strand at 1922.827 kb on + strand at 1922.828 kb on - strand at 1922.828 kb on - strand at 1922.828 kb on - strand at 1922.828 kb on - strand at 1922.878 kb on + strand at 1922.878 kb on + strand at 1922.878 kb on + strand at 1923.106 kb on + strand, within ABIE41_RS09275 at 1923.107 kb on - strand, within ABIE41_RS09275 at 1923.107 kb on - strand, within ABIE41_RS09275 at 1923.107 kb on - strand, within ABIE41_RS09275 at 1923.109 kb on + strand, within ABIE41_RS09275 at 1923.293 kb on + strand at 1923.294 kb on - strand at 1923.497 kb on + strand, within ABIE41_RS09280 at 1923.777 kb on - strand, within ABIE41_RS09280 at 1923.777 kb on - strand, within ABIE41_RS09280 at 1923.800 kb on + strand, within ABIE41_RS09280 at 1923.800 kb on + strand, within ABIE41_RS09280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 1,920,464 - ABIE41_RS09260 0.53 -0.5 1,920,464 - ABIE41_RS09260 0.53 -0.8 1,920,523 + ABIE41_RS09260 0.60 +0.1 1,920,524 - ABIE41_RS09260 0.61 +0.0 1,920,532 + ABIE41_RS09260 0.61 -1.7 1,920,533 - ABIE41_RS09260 0.62 -0.4 1,920,628 + ABIE41_RS09260 0.73 -0.4 1,920,815 - +0.7 1,921,134 + ABIE41_RS09265 0.49 +1.1 1,921,134 + ABIE41_RS09265 0.49 +0.3 1,921,135 - ABIE41_RS09265 0.49 -0.4 1,921,135 - ABIE41_RS09265 0.49 -0.2 1,921,135 - ABIE41_RS09265 0.49 -0.6 1,921,135 - ABIE41_RS09265 0.49 +0.4 1,921,135 - ABIE41_RS09265 0.49 +0.3 1,921,135 - ABIE41_RS09265 0.49 -0.2 1,921,253 + ABIE41_RS09265 0.74 -0.2 1,921,624 + ABIE41_RS09270 0.12 +1.0 1,921,625 - ABIE41_RS09270 0.13 -1.6 1,921,819 + ABIE41_RS09270 0.26 -0.8 1,921,819 + ABIE41_RS09270 0.26 -2.7 1,921,823 + ABIE41_RS09270 0.27 -1.4 1,921,824 - ABIE41_RS09270 0.27 -1.9 1,921,942 + ABIE41_RS09270 0.35 -0.5 1,921,942 + ABIE41_RS09270 0.35 -0.4 1,921,943 - ABIE41_RS09270 0.35 +0.9 1,922,080 + ABIE41_RS09270 0.45 -0.8 1,922,080 + ABIE41_RS09270 0.45 -0.3 1,922,080 + ABIE41_RS09270 0.45 -2.1 1,922,080 + ABIE41_RS09270 0.45 +0.4 1,922,080 + ABIE41_RS09270 0.45 -4.0 1,922,080 + ABIE41_RS09270 0.45 -0.5 1,922,081 - ABIE41_RS09270 0.45 -0.2 1,922,081 - ABIE41_RS09270 0.45 -0.5 1,922,180 - ABIE41_RS09270 0.52 -1.3 1,922,180 - ABIE41_RS09270 0.52 +0.5 1,922,528 - ABIE41_RS09270 0.77 -0.4 1,922,749 + +1.5 1,922,749 + -0.5 1,922,827 + +0.7 1,922,827 + -0.4 1,922,827 + +0.3 1,922,827 + -1.1 1,922,828 - -0.2 1,922,828 - +0.1 1,922,828 - +0.7 1,922,828 - -0.4 1,922,878 + -1.4 1,922,878 + -0.0 1,922,878 + -1.5 1,923,106 + ABIE41_RS09275 0.56 +0.2 1,923,107 - ABIE41_RS09275 0.56 -1.0 1,923,107 - ABIE41_RS09275 0.56 -0.3 1,923,107 - ABIE41_RS09275 0.56 +1.9 1,923,109 + ABIE41_RS09275 0.56 +0.5 1,923,293 + -0.5 1,923,294 - -1.0 1,923,497 + ABIE41_RS09280 0.17 +1.2 1,923,777 - ABIE41_RS09280 0.41 +0.7 1,923,777 - ABIE41_RS09280 0.41 -1.0 1,923,800 + ABIE41_RS09280 0.42 -0.8 1,923,800 + ABIE41_RS09280 0.42 +1.2
Or see this region's nucleotide sequence