Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS01985 and ABIE41_RS01990 are separated by 149 nucleotides ABIE41_RS01990 and ABIE41_RS01995 are separated by 38 nucleotides ABIE41_RS01995 and ABIE41_RS02000 are separated by 25 nucleotides ABIE41_RS02000 and ABIE41_RS02005 are separated by 33 nucleotides
ABIE41_RS01985: ABIE41_RS01985 - host attachment protein, at 398,356 to 398,760
_RS01985
ABIE41_RS01990: ABIE41_RS01990 - cytochrome c, at 398,910 to 399,281
_RS01990
ABIE41_RS01995: ABIE41_RS01995 - cytochrome c, at 399,320 to 399,685
_RS01995
ABIE41_RS02000: ABIE41_RS02000 - hypothetical protein, at 399,711 to 400,256
_RS02000
ABIE41_RS02005: ABIE41_RS02005 - hypothetical protein, at 400,290 to 400,523
_RS02005
Position (kb)
399
400 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 398.662 kb on + strand, within ABIE41_RS01985 at 398.663 kb on - strand, within ABIE41_RS01985 at 398.663 kb on - strand, within ABIE41_RS01985 at 398.667 kb on + strand, within ABIE41_RS01985 at 398.667 kb on + strand, within ABIE41_RS01985 at 398.667 kb on + strand, within ABIE41_RS01985 at 398.667 kb on + strand, within ABIE41_RS01985 at 398.667 kb on + strand, within ABIE41_RS01985 at 398.667 kb on + strand, within ABIE41_RS01985 at 398.667 kb on + strand, within ABIE41_RS01985 at 398.667 kb on + strand, within ABIE41_RS01985 at 398.667 kb on + strand, within ABIE41_RS01985 at 398.668 kb on - strand, within ABIE41_RS01985 at 398.668 kb on - strand, within ABIE41_RS01985 at 398.668 kb on - strand, within ABIE41_RS01985 at 398.847 kb on + strand at 398.847 kb on + strand at 398.847 kb on + strand at 398.848 kb on - strand at 398.848 kb on - strand at 399.048 kb on + strand, within ABIE41_RS01990 at 399.049 kb on - strand, within ABIE41_RS01990 at 399.049 kb on - strand, within ABIE41_RS01990 at 399.132 kb on + strand, within ABIE41_RS01990 at 399.240 kb on + strand, within ABIE41_RS01990 at 399.240 kb on + strand, within ABIE41_RS01990 at 399.240 kb on + strand, within ABIE41_RS01990 at 399.240 kb on + strand, within ABIE41_RS01990 at 399.240 kb on + strand, within ABIE41_RS01990 at 399.240 kb on + strand, within ABIE41_RS01990 at 399.240 kb on + strand, within ABIE41_RS01990 at 399.241 kb on - strand, within ABIE41_RS01990 at 399.241 kb on - strand, within ABIE41_RS01990 at 399.241 kb on - strand, within ABIE41_RS01990 at 399.241 kb on - strand, within ABIE41_RS01990 at 399.241 kb on - strand, within ABIE41_RS01990 at 399.242 kb on + strand, within ABIE41_RS01990 at 399.242 kb on + strand, within ABIE41_RS01990 at 399.243 kb on - strand, within ABIE41_RS01990 at 399.243 kb on - strand, within ABIE41_RS01990 at 399.659 kb on + strand at 399.659 kb on + strand at 399.659 kb on + strand at 399.660 kb on - strand at 399.970 kb on + strand, within ABIE41_RS02000 at 400.000 kb on + strand, within ABIE41_RS02000 at 400.000 kb on + strand, within ABIE41_RS02000 at 400.001 kb on - strand, within ABIE41_RS02000 at 400.375 kb on + strand, within ABIE41_RS02005 at 400.375 kb on + strand, within ABIE41_RS02005 at 400.376 kb on - strand, within ABIE41_RS02005 at 400.376 kb on - strand, within ABIE41_RS02005 at 400.376 kb on - strand, within ABIE41_RS02005 at 400.523 kb on + strand at 400.523 kb on + strand at 400.523 kb on + strand at 400.523 kb on + strand at 400.524 kb on - strand at 400.524 kb on - strand at 400.524 kb on - strand at 400.524 kb on - strand at 400.524 kb on - strand at 400.642 kb on + strand at 400.642 kb on + strand at 400.643 kb on - strand at 400.643 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 398,662 + ABIE41_RS01985 0.76 -1.3 398,663 - ABIE41_RS01985 0.76 -0.4 398,663 - ABIE41_RS01985 0.76 -0.2 398,667 + ABIE41_RS01985 0.77 +1.1 398,667 + ABIE41_RS01985 0.77 +1.5 398,667 + ABIE41_RS01985 0.77 +0.1 398,667 + ABIE41_RS01985 0.77 +0.3 398,667 + ABIE41_RS01985 0.77 -0.6 398,667 + ABIE41_RS01985 0.77 -0.1 398,667 + ABIE41_RS01985 0.77 +0.4 398,667 + ABIE41_RS01985 0.77 +0.2 398,667 + ABIE41_RS01985 0.77 -0.4 398,668 - ABIE41_RS01985 0.77 -1.0 398,668 - ABIE41_RS01985 0.77 +2.1 398,668 - ABIE41_RS01985 0.77 -0.6 398,847 + +0.5 398,847 + -0.1 398,847 + -0.1 398,848 - -1.4 398,848 - -1.5 399,048 + ABIE41_RS01990 0.37 +0.6 399,049 - ABIE41_RS01990 0.37 -0.7 399,049 - ABIE41_RS01990 0.37 +2.3 399,132 + ABIE41_RS01990 0.60 +0.3 399,240 + ABIE41_RS01990 0.89 -2.2 399,240 + ABIE41_RS01990 0.89 -1.2 399,240 + ABIE41_RS01990 0.89 +0.2 399,240 + ABIE41_RS01990 0.89 -1.1 399,240 + ABIE41_RS01990 0.89 +2.0 399,240 + ABIE41_RS01990 0.89 +2.5 399,240 + ABIE41_RS01990 0.89 -1.4 399,241 - ABIE41_RS01990 0.89 +0.0 399,241 - ABIE41_RS01990 0.89 +1.6 399,241 - ABIE41_RS01990 0.89 -0.2 399,241 - ABIE41_RS01990 0.89 -0.9 399,241 - ABIE41_RS01990 0.89 -0.6 399,242 + ABIE41_RS01990 0.89 -0.0 399,242 + ABIE41_RS01990 0.89 -2.7 399,243 - ABIE41_RS01990 0.90 -0.4 399,243 - ABIE41_RS01990 0.90 +1.5 399,659 + +0.4 399,659 + +0.3 399,659 + +1.2 399,660 - -0.5 399,970 + ABIE41_RS02000 0.47 -0.6 400,000 + ABIE41_RS02000 0.53 +0.2 400,000 + ABIE41_RS02000 0.53 -1.4 400,001 - ABIE41_RS02000 0.53 -0.3 400,375 + ABIE41_RS02005 0.36 +1.3 400,375 + ABIE41_RS02005 0.36 -0.3 400,376 - ABIE41_RS02005 0.37 -1.4 400,376 - ABIE41_RS02005 0.37 -3.4 400,376 - ABIE41_RS02005 0.37 +1.9 400,523 + -1.0 400,523 + -1.2 400,523 + +0.1 400,523 + -0.6 400,524 - -3.4 400,524 - +0.3 400,524 - +0.2 400,524 - -3.0 400,524 - -0.0 400,642 + -1.9 400,642 + -0.1 400,643 - -0.5 400,643 - +0.6
Or see this region's nucleotide sequence