Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS11715 and ABIE41_RS11720 are separated by 13 nucleotides ABIE41_RS11720 and ABIE41_RS11725 are separated by 61 nucleotides ABIE41_RS11725 and ABIE41_RS11730 are separated by 288 nucleotides
ABIE41_RS11715: ABIE41_RS11715 - class I SAM-dependent methyltransferase, at 2,403,521 to 2,404,120
_RS11715
ABIE41_RS11720: ABIE41_RS11720 - LysE family translocator, at 2,404,134 to 2,404,760
_RS11720
ABIE41_RS11725: ABIE41_RS11725 - CAP domain-containing protein, at 2,404,822 to 2,405,385
_RS11725
ABIE41_RS11730: ABIE41_RS11730 - signal recognition particle protein, at 2,405,674 to 2,407,260
_RS11730
Position (kb)
2404
2405
2406 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2404.065 kb on + strand at 2404.066 kb on - strand at 2404.086 kb on + strand at 2404.086 kb on + strand at 2404.087 kb on - strand at 2404.185 kb on + strand at 2404.186 kb on - strand at 2404.301 kb on - strand, within ABIE41_RS11720 at 2404.516 kb on + strand, within ABIE41_RS11720 at 2404.672 kb on + strand, within ABIE41_RS11720 at 2404.814 kb on - strand at 2404.925 kb on + strand, within ABIE41_RS11725 at 2405.042 kb on + strand, within ABIE41_RS11725 at 2405.043 kb on - strand, within ABIE41_RS11725 at 2405.139 kb on + strand, within ABIE41_RS11725 at 2405.140 kb on - strand, within ABIE41_RS11725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 2,404,065 + -0.9 2,404,066 - +0.5 2,404,086 + +3.1 2,404,086 + -0.1 2,404,087 - +2.5 2,404,185 + -2.6 2,404,186 - +1.3 2,404,301 - ABIE41_RS11720 0.27 -2.0 2,404,516 + ABIE41_RS11720 0.61 -2.5 2,404,672 + ABIE41_RS11720 0.86 +0.7 2,404,814 - +0.6 2,404,925 + ABIE41_RS11725 0.18 -0.1 2,405,042 + ABIE41_RS11725 0.39 -0.3 2,405,043 - ABIE41_RS11725 0.39 +1.5 2,405,139 + ABIE41_RS11725 0.56 +0.1 2,405,140 - ABIE41_RS11725 0.56 +0.0
Or see this region's nucleotide sequence