Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS07120 and ABIE41_RS07125 are separated by 398 nucleotides ABIE41_RS07125 and ABIE41_RS07130 are separated by 207 nucleotides
ABIE41_RS07120: ABIE41_RS07120 - EAL domain-containing protein, at 1,464,386 to 1,466,641
_RS07120
ABIE41_RS07125: ABIE41_RS07125 - HNH endonuclease, at 1,467,040 to 1,467,597
_RS07125
ABIE41_RS07130: ABIE41_RS07130 - 3-hydroxybutyryl-CoA dehydrogenase, at 1,467,805 to 1,468,701
_RS07130
Position (kb)
1467
1468 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1466.231 kb on + strand, within ABIE41_RS07120 at 1466.232 kb on - strand, within ABIE41_RS07120 at 1466.232 kb on - strand, within ABIE41_RS07120 at 1466.232 kb on - strand, within ABIE41_RS07120 at 1466.273 kb on + strand, within ABIE41_RS07120 at 1466.274 kb on - strand, within ABIE41_RS07120 at 1466.274 kb on - strand, within ABIE41_RS07120 at 1466.274 kb on - strand, within ABIE41_RS07120 at 1466.274 kb on - strand, within ABIE41_RS07120 at 1466.276 kb on - strand, within ABIE41_RS07120 at 1466.286 kb on - strand, within ABIE41_RS07120 at 1466.305 kb on + strand, within ABIE41_RS07120 at 1466.305 kb on + strand, within ABIE41_RS07120 at 1466.305 kb on + strand, within ABIE41_RS07120 at 1466.306 kb on - strand, within ABIE41_RS07120 at 1466.306 kb on - strand, within ABIE41_RS07120 at 1466.362 kb on + strand, within ABIE41_RS07120 at 1466.362 kb on + strand, within ABIE41_RS07120 at 1466.373 kb on + strand, within ABIE41_RS07120 at 1466.374 kb on - strand, within ABIE41_RS07120 at 1466.374 kb on - strand, within ABIE41_RS07120 at 1466.382 kb on + strand, within ABIE41_RS07120 at 1466.382 kb on + strand, within ABIE41_RS07120 at 1466.382 kb on + strand, within ABIE41_RS07120 at 1466.382 kb on + strand, within ABIE41_RS07120 at 1466.383 kb on - strand, within ABIE41_RS07120 at 1466.383 kb on - strand, within ABIE41_RS07120 at 1466.383 kb on - strand, within ABIE41_RS07120 at 1466.480 kb on + strand at 1466.480 kb on + strand at 1466.481 kb on - strand at 1466.501 kb on + strand at 1466.502 kb on - strand at 1466.502 kb on - strand at 1466.502 kb on - strand at 1466.502 kb on - strand at 1466.502 kb on - strand at 1466.547 kb on + strand at 1466.569 kb on + strand at 1466.569 kb on + strand at 1466.570 kb on - strand at 1466.570 kb on - strand at 1466.651 kb on + strand at 1466.651 kb on + strand at 1466.651 kb on + strand at 1466.651 kb on + strand at 1466.652 kb on - strand at 1466.652 kb on - strand at 1466.652 kb on - strand at 1466.652 kb on - strand at 1466.652 kb on - strand at 1466.652 kb on - strand at 1466.652 kb on - strand at 1466.706 kb on - strand at 1466.706 kb on - strand at 1466.738 kb on + strand at 1466.738 kb on + strand at 1466.738 kb on + strand at 1466.739 kb on - strand at 1466.739 kb on - strand at 1466.739 kb on - strand at 1466.739 kb on - strand at 1466.776 kb on + strand at 1466.776 kb on + strand at 1466.776 kb on + strand at 1466.776 kb on + strand at 1466.776 kb on + strand at 1466.776 kb on + strand at 1466.796 kb on + strand at 1466.796 kb on + strand at 1466.796 kb on + strand at 1466.797 kb on - strand at 1466.797 kb on - strand at 1466.797 kb on - strand at 1466.813 kb on + strand at 1466.813 kb on + strand at 1466.813 kb on + strand at 1466.948 kb on + strand at 1466.948 kb on + strand at 1466.949 kb on - strand at 1466.949 kb on - strand at 1466.979 kb on + strand at 1466.979 kb on + strand at 1466.979 kb on + strand at 1466.980 kb on - strand at 1466.988 kb on + strand at 1466.988 kb on + strand at 1466.989 kb on - strand at 1466.989 kb on - strand at 1466.989 kb on - strand at 1466.989 kb on - strand at 1466.989 kb on - strand at 1466.989 kb on - strand at 1466.990 kb on + strand at 1467.008 kb on + strand at 1467.008 kb on + strand at 1467.009 kb on - strand at 1467.065 kb on + strand at 1467.065 kb on + strand at 1467.072 kb on - strand at 1467.072 kb on - strand at 1467.072 kb on - strand at 1467.095 kb on + strand at 1467.095 kb on + strand at 1467.095 kb on + strand at 1467.095 kb on + strand at 1467.095 kb on + strand at 1467.095 kb on + strand at 1467.095 kb on + strand at 1467.095 kb on + strand at 1467.096 kb on - strand, within ABIE41_RS07125 at 1467.096 kb on - strand, within ABIE41_RS07125 at 1467.096 kb on - strand, within ABIE41_RS07125 at 1467.096 kb on - strand, within ABIE41_RS07125 at 1467.096 kb on - strand, within ABIE41_RS07125 at 1467.150 kb on - strand, within ABIE41_RS07125 at 1467.192 kb on + strand, within ABIE41_RS07125 at 1467.192 kb on + strand, within ABIE41_RS07125 at 1467.193 kb on - strand, within ABIE41_RS07125 at 1467.311 kb on + strand, within ABIE41_RS07125 at 1467.311 kb on + strand, within ABIE41_RS07125 at 1467.311 kb on + strand, within ABIE41_RS07125 at 1467.311 kb on + strand, within ABIE41_RS07125 at 1467.312 kb on - strand, within ABIE41_RS07125 at 1467.312 kb on - strand, within ABIE41_RS07125 at 1467.383 kb on + strand, within ABIE41_RS07125 at 1467.384 kb on - strand, within ABIE41_RS07125 at 1467.384 kb on - strand, within ABIE41_RS07125 at 1467.384 kb on - strand, within ABIE41_RS07125 at 1467.384 kb on - strand, within ABIE41_RS07125 at 1467.384 kb on - strand, within ABIE41_RS07125 at 1467.462 kb on + strand, within ABIE41_RS07125 at 1467.462 kb on + strand, within ABIE41_RS07125 at 1467.462 kb on + strand, within ABIE41_RS07125 at 1467.463 kb on - strand, within ABIE41_RS07125 at 1467.524 kb on + strand, within ABIE41_RS07125 at 1467.527 kb on + strand, within ABIE41_RS07125 at 1467.528 kb on - strand, within ABIE41_RS07125 at 1467.528 kb on - strand, within ABIE41_RS07125 at 1467.528 kb on - strand, within ABIE41_RS07125 at 1467.528 kb on - strand, within ABIE41_RS07125 at 1467.667 kb on + strand at 1467.667 kb on + strand at 1467.667 kb on + strand at 1467.667 kb on + strand at 1467.667 kb on + strand at 1467.667 kb on + strand at 1467.667 kb on + strand at 1467.667 kb on + strand at 1467.668 kb on - strand at 1467.668 kb on - strand at 1467.668 kb on - strand at 1467.668 kb on - strand at 1467.668 kb on - strand at 1467.668 kb on - strand at 1467.837 kb on - strand at 1467.887 kb on + strand at 1467.887 kb on + strand at 1467.887 kb on + strand at 1467.914 kb on + strand, within ABIE41_RS07130 at 1468.074 kb on + strand, within ABIE41_RS07130 at 1468.077 kb on - strand, within ABIE41_RS07130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 1,466,231 + ABIE41_RS07120 0.82 -0.1 1,466,232 - ABIE41_RS07120 0.82 -1.1 1,466,232 - ABIE41_RS07120 0.82 -0.7 1,466,232 - ABIE41_RS07120 0.82 +2.6 1,466,273 + ABIE41_RS07120 0.84 -1.4 1,466,274 - ABIE41_RS07120 0.84 -1.4 1,466,274 - ABIE41_RS07120 0.84 +1.1 1,466,274 - ABIE41_RS07120 0.84 +0.4 1,466,274 - ABIE41_RS07120 0.84 -1.0 1,466,276 - ABIE41_RS07120 0.84 -0.0 1,466,286 - ABIE41_RS07120 0.84 +0.2 1,466,305 + ABIE41_RS07120 0.85 -0.0 1,466,305 + ABIE41_RS07120 0.85 +0.4 1,466,305 + ABIE41_RS07120 0.85 +0.7 1,466,306 - ABIE41_RS07120 0.85 -0.8 1,466,306 - ABIE41_RS07120 0.85 +1.5 1,466,362 + ABIE41_RS07120 0.88 +0.1 1,466,362 + ABIE41_RS07120 0.88 +0.9 1,466,373 + ABIE41_RS07120 0.88 +0.3 1,466,374 - ABIE41_RS07120 0.88 +0.2 1,466,374 - ABIE41_RS07120 0.88 +0.3 1,466,382 + ABIE41_RS07120 0.88 -1.2 1,466,382 + ABIE41_RS07120 0.88 -0.3 1,466,382 + ABIE41_RS07120 0.88 +0.2 1,466,382 + ABIE41_RS07120 0.88 +0.3 1,466,383 - ABIE41_RS07120 0.89 -1.4 1,466,383 - ABIE41_RS07120 0.89 -0.2 1,466,383 - ABIE41_RS07120 0.89 -1.7 1,466,480 + +0.1 1,466,480 + -0.4 1,466,481 - -0.9 1,466,501 + -0.6 1,466,502 - -0.6 1,466,502 - -0.0 1,466,502 - +0.8 1,466,502 - -0.2 1,466,502 - -0.3 1,466,547 + -1.4 1,466,569 + -2.7 1,466,569 + +0.2 1,466,570 - -0.6 1,466,570 - +0.2 1,466,651 + +2.2 1,466,651 + +0.4 1,466,651 + -0.3 1,466,651 + -0.6 1,466,652 - -2.8 1,466,652 - +1.0 1,466,652 - +0.5 1,466,652 - -2.0 1,466,652 - +0.1 1,466,652 - +0.9 1,466,652 - +0.4 1,466,706 - +0.1 1,466,706 - +1.2 1,466,738 + -1.2 1,466,738 + -0.6 1,466,738 + +3.5 1,466,739 - -0.6 1,466,739 - +0.0 1,466,739 - +0.2 1,466,739 - -2.8 1,466,776 + -0.3 1,466,776 + -1.9 1,466,776 + +0.8 1,466,776 + +0.6 1,466,776 + -0.8 1,466,776 + -0.6 1,466,796 + -0.0 1,466,796 + -0.5 1,466,796 + -2.7 1,466,797 - +0.3 1,466,797 - +2.6 1,466,797 - -1.7 1,466,813 + -2.3 1,466,813 + -0.6 1,466,813 + -2.3 1,466,948 + +0.0 1,466,948 + +1.0 1,466,949 - -1.0 1,466,949 - -0.4 1,466,979 + -0.4 1,466,979 + -0.1 1,466,979 + +0.8 1,466,980 - -2.2 1,466,988 + +0.9 1,466,988 + -0.5 1,466,989 - -0.0 1,466,989 - -2.8 1,466,989 - -1.0 1,466,989 - -0.1 1,466,989 - +2.0 1,466,989 - -0.6 1,466,990 + +0.1 1,467,008 + +0.2 1,467,008 + +0.8 1,467,009 - +0.0 1,467,065 + -3.7 1,467,065 + +0.2 1,467,072 - -1.1 1,467,072 - +0.6 1,467,072 - -1.1 1,467,095 + +0.2 1,467,095 + -0.8 1,467,095 + -0.6 1,467,095 + -0.0 1,467,095 + -0.2 1,467,095 + -1.2 1,467,095 + -0.2 1,467,095 + -0.1 1,467,096 - ABIE41_RS07125 0.10 +0.9 1,467,096 - ABIE41_RS07125 0.10 -1.3 1,467,096 - ABIE41_RS07125 0.10 -0.3 1,467,096 - ABIE41_RS07125 0.10 -1.4 1,467,096 - ABIE41_RS07125 0.10 +0.8 1,467,150 - ABIE41_RS07125 0.20 -0.7 1,467,192 + ABIE41_RS07125 0.27 +0.5 1,467,192 + ABIE41_RS07125 0.27 -0.2 1,467,193 - ABIE41_RS07125 0.27 +3.9 1,467,311 + ABIE41_RS07125 0.49 +1.5 1,467,311 + ABIE41_RS07125 0.49 -1.7 1,467,311 + ABIE41_RS07125 0.49 -1.4 1,467,311 + ABIE41_RS07125 0.49 -1.4 1,467,312 - ABIE41_RS07125 0.49 +0.0 1,467,312 - ABIE41_RS07125 0.49 -1.8 1,467,383 + ABIE41_RS07125 0.61 +0.3 1,467,384 - ABIE41_RS07125 0.62 -0.8 1,467,384 - ABIE41_RS07125 0.62 -0.4 1,467,384 - ABIE41_RS07125 0.62 +0.6 1,467,384 - ABIE41_RS07125 0.62 +0.7 1,467,384 - ABIE41_RS07125 0.62 -0.8 1,467,462 + ABIE41_RS07125 0.76 -0.4 1,467,462 + ABIE41_RS07125 0.76 -0.3 1,467,462 + ABIE41_RS07125 0.76 -0.6 1,467,463 - ABIE41_RS07125 0.76 -0.7 1,467,524 + ABIE41_RS07125 0.87 +0.4 1,467,527 + ABIE41_RS07125 0.87 -0.0 1,467,528 - ABIE41_RS07125 0.87 -1.5 1,467,528 - ABIE41_RS07125 0.87 -0.1 1,467,528 - ABIE41_RS07125 0.87 -0.2 1,467,528 - ABIE41_RS07125 0.87 -0.0 1,467,667 + +1.9 1,467,667 + -2.6 1,467,667 + +1.2 1,467,667 + +1.4 1,467,667 + +0.7 1,467,667 + +0.2 1,467,667 + +0.2 1,467,667 + +0.4 1,467,668 - -3.4 1,467,668 - -1.9 1,467,668 - +0.3 1,467,668 - -2.2 1,467,668 - +0.8 1,467,668 - -0.5 1,467,837 - -1.0 1,467,887 + -3.2 1,467,887 + -1.7 1,467,887 + -1.5 1,467,914 + ABIE41_RS07130 0.12 -1.1 1,468,074 + ABIE41_RS07130 0.30 -1.9 1,468,077 - ABIE41_RS07130 0.30 +0.0
Or see this region's nucleotide sequence