Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS05335 and ABIE41_RS05340 are separated by 142 nucleotides ABIE41_RS05340 and ABIE41_RS05345 are separated by 98 nucleotides ABIE41_RS05345 and ABIE41_RS05350 are separated by 136 nucleotides
ABIE41_RS05335: ABIE41_RS05335 - alpha/beta hydrolase, at 1,104,770 to 1,105,657
_RS05335
ABIE41_RS05340: ABIE41_RS05340 - helix-turn-helix domain-containing protein, at 1,105,800 to 1,106,219
_RS05340
ABIE41_RS05345: ABIE41_RS05345 - cupin domain-containing protein, at 1,106,318 to 1,106,869
_RS05345
ABIE41_RS05350: ABIE41_RS05350 - aspartate aminotransferase family protein, at 1,107,006 to 1,108,355
_RS05350
Position (kb)
1105
1106
1107 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1105.131 kb on + strand, within ABIE41_RS05335 at 1105.131 kb on + strand, within ABIE41_RS05335 at 1105.131 kb on + strand, within ABIE41_RS05335 at 1105.132 kb on - strand, within ABIE41_RS05335 at 1105.132 kb on - strand, within ABIE41_RS05335 at 1105.132 kb on - strand, within ABIE41_RS05335 at 1105.132 kb on - strand, within ABIE41_RS05335 at 1105.132 kb on - strand, within ABIE41_RS05335 at 1105.132 kb on - strand, within ABIE41_RS05335 at 1105.201 kb on - strand, within ABIE41_RS05335 at 1105.323 kb on + strand, within ABIE41_RS05335 at 1105.323 kb on + strand, within ABIE41_RS05335 at 1105.324 kb on - strand, within ABIE41_RS05335 at 1105.324 kb on - strand, within ABIE41_RS05335 at 1105.687 kb on + strand at 1105.688 kb on - strand at 1106.076 kb on + strand, within ABIE41_RS05340 at 1106.076 kb on + strand, within ABIE41_RS05340 at 1106.077 kb on - strand, within ABIE41_RS05340 at 1106.077 kb on - strand, within ABIE41_RS05340 at 1106.077 kb on - strand, within ABIE41_RS05340 at 1106.158 kb on + strand, within ABIE41_RS05340 at 1106.158 kb on + strand, within ABIE41_RS05340 at 1106.159 kb on - strand, within ABIE41_RS05340 at 1106.159 kb on - strand, within ABIE41_RS05340 at 1106.161 kb on + strand, within ABIE41_RS05340 at 1106.162 kb on - strand, within ABIE41_RS05340 at 1106.246 kb on + strand at 1106.246 kb on + strand at 1106.246 kb on + strand at 1106.246 kb on + strand at 1106.246 kb on + strand at 1106.246 kb on + strand at 1106.247 kb on - strand at 1106.247 kb on - strand at 1106.247 kb on - strand at 1106.247 kb on - strand at 1106.247 kb on - strand at 1106.247 kb on - strand at 1106.264 kb on + strand at 1106.265 kb on - strand at 1106.265 kb on - strand at 1106.265 kb on - strand at 1106.265 kb on - strand at 1106.345 kb on + strand at 1106.345 kb on + strand at 1106.345 kb on + strand at 1106.346 kb on - strand at 1106.346 kb on - strand at 1106.406 kb on + strand, within ABIE41_RS05345 at 1106.407 kb on - strand, within ABIE41_RS05345 at 1106.407 kb on - strand, within ABIE41_RS05345 at 1106.409 kb on + strand, within ABIE41_RS05345 at 1106.410 kb on - strand, within ABIE41_RS05345 at 1106.410 kb on - strand, within ABIE41_RS05345 at 1106.538 kb on + strand, within ABIE41_RS05345 at 1106.538 kb on + strand, within ABIE41_RS05345 at 1106.539 kb on - strand, within ABIE41_RS05345 at 1106.539 kb on - strand, within ABIE41_RS05345 at 1106.595 kb on + strand, within ABIE41_RS05345 at 1106.595 kb on + strand, within ABIE41_RS05345 at 1106.595 kb on + strand, within ABIE41_RS05345 at 1106.641 kb on - strand, within ABIE41_RS05345 at 1106.835 kb on + strand at 1107.060 kb on + strand at 1107.060 kb on + strand at 1107.060 kb on + strand at 1107.060 kb on + strand at 1107.061 kb on - strand at 1107.061 kb on - strand at 1107.061 kb on - strand at 1107.135 kb on + strand at 1107.135 kb on + strand at 1107.135 kb on + strand at 1107.136 kb on - strand at 1107.136 kb on - strand at 1107.136 kb on - strand at 1107.136 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 1,105,131 + ABIE41_RS05335 0.41 +1.1 1,105,131 + ABIE41_RS05335 0.41 +1.3 1,105,131 + ABIE41_RS05335 0.41 -0.9 1,105,132 - ABIE41_RS05335 0.41 +0.8 1,105,132 - ABIE41_RS05335 0.41 -1.4 1,105,132 - ABIE41_RS05335 0.41 -1.6 1,105,132 - ABIE41_RS05335 0.41 -0.1 1,105,132 - ABIE41_RS05335 0.41 +0.8 1,105,132 - ABIE41_RS05335 0.41 +1.0 1,105,201 - ABIE41_RS05335 0.49 -0.0 1,105,323 + ABIE41_RS05335 0.62 +0.5 1,105,323 + ABIE41_RS05335 0.62 +0.6 1,105,324 - ABIE41_RS05335 0.62 -2.8 1,105,324 - ABIE41_RS05335 0.62 +2.0 1,105,687 + +1.7 1,105,688 - -1.0 1,106,076 + ABIE41_RS05340 0.66 +0.5 1,106,076 + ABIE41_RS05340 0.66 +0.9 1,106,077 - ABIE41_RS05340 0.66 +0.0 1,106,077 - ABIE41_RS05340 0.66 -1.4 1,106,077 - ABIE41_RS05340 0.66 +0.4 1,106,158 + ABIE41_RS05340 0.85 +0.9 1,106,158 + ABIE41_RS05340 0.85 -0.0 1,106,159 - ABIE41_RS05340 0.85 -0.5 1,106,159 - ABIE41_RS05340 0.85 -1.6 1,106,161 + ABIE41_RS05340 0.86 +0.2 1,106,162 - ABIE41_RS05340 0.86 +0.5 1,106,246 + +0.3 1,106,246 + +1.1 1,106,246 + +0.5 1,106,246 + +2.1 1,106,246 + +0.7 1,106,246 + -1.7 1,106,247 - -2.2 1,106,247 - -0.4 1,106,247 - +1.2 1,106,247 - +0.5 1,106,247 - +0.8 1,106,247 - -2.2 1,106,264 + +1.6 1,106,265 - +1.6 1,106,265 - +1.0 1,106,265 - -0.4 1,106,265 - +1.0 1,106,345 + -1.2 1,106,345 + +0.2 1,106,345 + -2.8 1,106,346 - +1.8 1,106,346 - -2.8 1,106,406 + ABIE41_RS05345 0.16 +0.7 1,106,407 - ABIE41_RS05345 0.16 +2.0 1,106,407 - ABIE41_RS05345 0.16 +0.3 1,106,409 + ABIE41_RS05345 0.16 +0.3 1,106,410 - ABIE41_RS05345 0.17 -0.4 1,106,410 - ABIE41_RS05345 0.17 -1.0 1,106,538 + ABIE41_RS05345 0.40 +2.3 1,106,538 + ABIE41_RS05345 0.40 +0.5 1,106,539 - ABIE41_RS05345 0.40 -0.3 1,106,539 - ABIE41_RS05345 0.40 -0.3 1,106,595 + ABIE41_RS05345 0.50 +1.2 1,106,595 + ABIE41_RS05345 0.50 +1.0 1,106,595 + ABIE41_RS05345 0.50 +0.6 1,106,641 - ABIE41_RS05345 0.59 +0.8 1,106,835 + -0.2 1,107,060 + +0.0 1,107,060 + +0.6 1,107,060 + +0.4 1,107,060 + +0.9 1,107,061 - -0.8 1,107,061 - -0.7 1,107,061 - -0.4 1,107,135 + -1.0 1,107,135 + -0.1 1,107,135 + +0.2 1,107,136 - +1.4 1,107,136 - +1.2 1,107,136 - +1.6 1,107,136 - +0.6
Or see this region's nucleotide sequence