Experiment: R2A with 3-octanone 5.3129 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS04850 and ABIE41_RS04855 overlap by 4 nucleotides ABIE41_RS04855 and ABIE41_RS04860 are separated by 16 nucleotides ABIE41_RS04860 and ABIE41_RS04865 are separated by 232 nucleotides ABIE41_RS04865 and ABIE41_RS04870 are separated by 4 nucleotides
ABIE41_RS04850: ABIE41_RS04850 - MbcA/ParS/Xre antitoxin family protein, at 1,012,815 to 1,013,225
_RS04850
ABIE41_RS04855: ABIE41_RS04855 - RES family NAD+ phosphorylase, at 1,013,222 to 1,013,959
_RS04855
ABIE41_RS04860: ABIE41_RS04860 - SDR family NAD(P)-dependent oxidoreductase, at 1,013,976 to 1,014,761
_RS04860
ABIE41_RS04865: ABIE41_RS04865 - YkvA family protein, at 1,014,994 to 1,015,329
_RS04865
ABIE41_RS04870: ABIE41_RS04870 - NADPH:quinone oxidoreductase family protein, at 1,015,334 to 1,016,308
_RS04870
Position (kb)
1013
1014
1015 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1012.979 kb on - strand, within ABIE41_RS04850 at 1013.097 kb on + strand, within ABIE41_RS04850 at 1013.098 kb on - strand, within ABIE41_RS04850 at 1013.098 kb on - strand, within ABIE41_RS04850 at 1013.197 kb on - strand at 1013.525 kb on + strand, within ABIE41_RS04855 at 1013.525 kb on + strand, within ABIE41_RS04855 at 1013.714 kb on + strand, within ABIE41_RS04855 at 1013.715 kb on - strand, within ABIE41_RS04855 at 1013.715 kb on - strand, within ABIE41_RS04855 at 1013.715 kb on - strand, within ABIE41_RS04855 at 1013.715 kb on - strand, within ABIE41_RS04855 at 1013.715 kb on - strand, within ABIE41_RS04855 at 1013.819 kb on + strand, within ABIE41_RS04855 at 1013.820 kb on - strand, within ABIE41_RS04855 at 1013.864 kb on + strand, within ABIE41_RS04855 at 1013.865 kb on - strand, within ABIE41_RS04855 at 1013.957 kb on + strand at 1013.957 kb on + strand at 1013.957 kb on + strand at 1013.957 kb on + strand at 1013.958 kb on - strand at 1014.067 kb on + strand, within ABIE41_RS04860 at 1014.263 kb on - strand, within ABIE41_RS04860 at 1014.798 kb on + strand at 1014.799 kb on - strand at 1015.174 kb on + strand, within ABIE41_RS04865 at 1015.387 kb on - strand at 1015.387 kb on - strand at 1015.387 kb on - strand at 1015.387 kb on - strand at 1015.387 kb on - strand at 1015.474 kb on + strand, within ABIE41_RS04870 at 1015.475 kb on - strand, within ABIE41_RS04870 at 1015.608 kb on + strand, within ABIE41_RS04870 at 1015.608 kb on + strand, within ABIE41_RS04870 at 1015.609 kb on - strand, within ABIE41_RS04870 at 1015.609 kb on - strand, within ABIE41_RS04870 at 1015.671 kb on + strand, within ABIE41_RS04870 at 1015.671 kb on + strand, within ABIE41_RS04870 at 1015.672 kb on - strand, within ABIE41_RS04870
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 3-octanone 5.3129 mM remove 1,012,979 - ABIE41_RS04850 0.40 -2.9 1,013,097 + ABIE41_RS04850 0.69 -0.8 1,013,098 - ABIE41_RS04850 0.69 +0.1 1,013,098 - ABIE41_RS04850 0.69 +0.5 1,013,197 - -2.3 1,013,525 + ABIE41_RS04855 0.41 +0.1 1,013,525 + ABIE41_RS04855 0.41 -1.9 1,013,714 + ABIE41_RS04855 0.67 -0.5 1,013,715 - ABIE41_RS04855 0.67 -0.1 1,013,715 - ABIE41_RS04855 0.67 -1.0 1,013,715 - ABIE41_RS04855 0.67 -0.8 1,013,715 - ABIE41_RS04855 0.67 -0.1 1,013,715 - ABIE41_RS04855 0.67 -1.0 1,013,819 + ABIE41_RS04855 0.81 -2.5 1,013,820 - ABIE41_RS04855 0.81 +0.1 1,013,864 + ABIE41_RS04855 0.87 -0.4 1,013,865 - ABIE41_RS04855 0.87 +1.4 1,013,957 + +0.4 1,013,957 + -0.1 1,013,957 + -0.1 1,013,957 + -2.5 1,013,958 - +1.7 1,014,067 + ABIE41_RS04860 0.12 +2.6 1,014,263 - ABIE41_RS04860 0.37 -1.8 1,014,798 + +4.1 1,014,799 - -0.1 1,015,174 + ABIE41_RS04865 0.54 +1.2 1,015,387 - +0.7 1,015,387 - -1.7 1,015,387 - -0.2 1,015,387 - +2.2 1,015,387 - +0.0 1,015,474 + ABIE41_RS04870 0.14 +0.1 1,015,475 - ABIE41_RS04870 0.14 +1.7 1,015,608 + ABIE41_RS04870 0.28 -0.4 1,015,608 + ABIE41_RS04870 0.28 -0.6 1,015,609 - ABIE41_RS04870 0.28 +1.0 1,015,609 - ABIE41_RS04870 0.28 -0.3 1,015,671 + ABIE41_RS04870 0.35 -0.3 1,015,671 + ABIE41_RS04870 0.35 -1.6 1,015,672 - ABIE41_RS04870 0.35 +0.2
Or see this region's nucleotide sequence