Experiment: R2A with 2-pentyl-furan 21.3684 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS04130 and ABIE41_RS04135 are separated by 69 nucleotides ABIE41_RS04135 and ABIE41_RS04140 are separated by 62 nucleotides ABIE41_RS04140 and ABIE41_RS04145 are separated by 241 nucleotides
ABIE41_RS04130: ABIE41_RS04130 - diguanylate cyclase, at 853,085 to 854,290
_RS04130
ABIE41_RS04135: ABIE41_RS04135 - glutaminase, at 854,360 to 855,292
_RS04135
ABIE41_RS04140: ABIE41_RS04140 - hypothetical protein, at 855,355 to 855,798
_RS04140
ABIE41_RS04145: ABIE41_RS04145 - methyl-accepting chemotaxis protein, at 856,040 to 857,782
_RS04145
Position (kb)
854
855
856 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 853.394 kb on + strand, within ABIE41_RS04130 at 853.394 kb on + strand, within ABIE41_RS04130 at 853.394 kb on + strand, within ABIE41_RS04130 at 853.395 kb on - strand, within ABIE41_RS04130 at 853.395 kb on - strand, within ABIE41_RS04130 at 853.395 kb on - strand, within ABIE41_RS04130 at 853.395 kb on - strand, within ABIE41_RS04130 at 853.434 kb on - strand, within ABIE41_RS04130 at 853.649 kb on + strand, within ABIE41_RS04130 at 853.649 kb on + strand, within ABIE41_RS04130 at 853.649 kb on + strand, within ABIE41_RS04130 at 853.650 kb on - strand, within ABIE41_RS04130 at 853.650 kb on - strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.656 kb on - strand, within ABIE41_RS04130 at 853.656 kb on - strand, within ABIE41_RS04130 at 853.656 kb on - strand, within ABIE41_RS04130 at 853.656 kb on - strand, within ABIE41_RS04130 at 853.656 kb on - strand, within ABIE41_RS04130 at 853.656 kb on - strand, within ABIE41_RS04130 at 853.811 kb on + strand, within ABIE41_RS04130 at 853.811 kb on + strand, within ABIE41_RS04130 at 853.841 kb on + strand, within ABIE41_RS04130 at 853.841 kb on + strand, within ABIE41_RS04130 at 853.841 kb on + strand, within ABIE41_RS04130 at 853.841 kb on + strand, within ABIE41_RS04130 at 853.842 kb on - strand, within ABIE41_RS04130 at 853.842 kb on - strand, within ABIE41_RS04130 at 854.064 kb on - strand, within ABIE41_RS04130 at 854.064 kb on - strand, within ABIE41_RS04130 at 854.177 kb on + strand at 854.288 kb on + strand at 854.288 kb on + strand at 854.288 kb on + strand at 854.288 kb on + strand at 854.288 kb on + strand at 854.438 kb on + strand at 854.438 kb on + strand at 854.438 kb on + strand at 854.438 kb on + strand at 854.438 kb on + strand at 854.438 kb on + strand at 854.438 kb on + strand at 854.438 kb on + strand at 854.438 kb on + strand at 854.439 kb on - strand at 854.440 kb on + strand at 854.876 kb on + strand, within ABIE41_RS04135 at 854.876 kb on + strand, within ABIE41_RS04135 at 854.876 kb on + strand, within ABIE41_RS04135 at 854.877 kb on - strand, within ABIE41_RS04135 at 854.877 kb on - strand, within ABIE41_RS04135 at 854.877 kb on - strand, within ABIE41_RS04135 at 854.877 kb on - strand, within ABIE41_RS04135 at 854.877 kb on - strand, within ABIE41_RS04135 at 854.930 kb on + strand, within ABIE41_RS04135 at 854.930 kb on + strand, within ABIE41_RS04135 at 854.930 kb on + strand, within ABIE41_RS04135 at 854.931 kb on - strand, within ABIE41_RS04135 at 854.931 kb on - strand, within ABIE41_RS04135 at 854.988 kb on - strand, within ABIE41_RS04135 at 855.526 kb on + strand, within ABIE41_RS04140 at 855.526 kb on + strand, within ABIE41_RS04140 at 855.526 kb on + strand, within ABIE41_RS04140 at 855.527 kb on - strand, within ABIE41_RS04140 at 855.527 kb on - strand, within ABIE41_RS04140 at 855.527 kb on - strand, within ABIE41_RS04140 at 855.527 kb on - strand, within ABIE41_RS04140 at 855.527 kb on - strand, within ABIE41_RS04140 at 855.527 kb on - strand, within ABIE41_RS04140 at 855.529 kb on - strand, within ABIE41_RS04140 at 855.886 kb on + strand at 855.886 kb on + strand at 855.887 kb on - strand at 855.887 kb on - strand at 855.887 kb on - strand at 855.888 kb on + strand at 855.889 kb on - strand at 856.023 kb on - strand at 856.023 kb on - strand at 856.054 kb on + strand at 856.055 kb on - strand at 856.055 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 2-pentyl-furan 21.3684 mM remove 853,394 + ABIE41_RS04130 0.26 -0.9 853,394 + ABIE41_RS04130 0.26 -1.8 853,394 + ABIE41_RS04130 0.26 +0.6 853,395 - ABIE41_RS04130 0.26 +2.7 853,395 - ABIE41_RS04130 0.26 +0.4 853,395 - ABIE41_RS04130 0.26 +0.7 853,395 - ABIE41_RS04130 0.26 +1.3 853,434 - ABIE41_RS04130 0.29 -0.1 853,649 + ABIE41_RS04130 0.47 -0.9 853,649 + ABIE41_RS04130 0.47 -0.4 853,649 + ABIE41_RS04130 0.47 +0.2 853,650 - ABIE41_RS04130 0.47 -0.2 853,650 - ABIE41_RS04130 0.47 -0.2 853,655 + ABIE41_RS04130 0.47 -0.3 853,655 + ABIE41_RS04130 0.47 -1.3 853,655 + ABIE41_RS04130 0.47 +0.4 853,655 + ABIE41_RS04130 0.47 -0.4 853,655 + ABIE41_RS04130 0.47 -1.1 853,655 + ABIE41_RS04130 0.47 +0.3 853,655 + ABIE41_RS04130 0.47 -0.3 853,655 + ABIE41_RS04130 0.47 -0.8 853,655 + ABIE41_RS04130 0.47 +2.7 853,655 + ABIE41_RS04130 0.47 +0.5 853,655 + ABIE41_RS04130 0.47 +1.0 853,656 - ABIE41_RS04130 0.47 -2.3 853,656 - ABIE41_RS04130 0.47 -0.9 853,656 - ABIE41_RS04130 0.47 -2.5 853,656 - ABIE41_RS04130 0.47 -0.6 853,656 - ABIE41_RS04130 0.47 -2.1 853,656 - ABIE41_RS04130 0.47 -1.7 853,811 + ABIE41_RS04130 0.60 -0.3 853,811 + ABIE41_RS04130 0.60 -0.4 853,841 + ABIE41_RS04130 0.63 +1.3 853,841 + ABIE41_RS04130 0.63 -0.2 853,841 + ABIE41_RS04130 0.63 -1.0 853,841 + ABIE41_RS04130 0.63 -2.3 853,842 - ABIE41_RS04130 0.63 -0.6 853,842 - ABIE41_RS04130 0.63 -0.6 854,064 - ABIE41_RS04130 0.81 +0.0 854,064 - ABIE41_RS04130 0.81 +0.2 854,177 + -2.6 854,288 + -1.0 854,288 + -0.2 854,288 + +0.3 854,288 + +1.1 854,288 + -0.2 854,438 + -0.4 854,438 + +0.6 854,438 + -0.3 854,438 + -0.7 854,438 + +0.8 854,438 + +0.4 854,438 + +0.2 854,438 + +0.9 854,438 + -0.4 854,439 - -0.9 854,440 + -1.7 854,876 + ABIE41_RS04135 0.55 +0.4 854,876 + ABIE41_RS04135 0.55 -1.9 854,876 + ABIE41_RS04135 0.55 -1.7 854,877 - ABIE41_RS04135 0.55 -0.6 854,877 - ABIE41_RS04135 0.55 +0.4 854,877 - ABIE41_RS04135 0.55 +0.7 854,877 - ABIE41_RS04135 0.55 -0.0 854,877 - ABIE41_RS04135 0.55 -2.3 854,930 + ABIE41_RS04135 0.61 -2.2 854,930 + ABIE41_RS04135 0.61 -0.3 854,930 + ABIE41_RS04135 0.61 -1.2 854,931 - ABIE41_RS04135 0.61 +1.2 854,931 - ABIE41_RS04135 0.61 -0.2 854,988 - ABIE41_RS04135 0.67 -0.0 855,526 + ABIE41_RS04140 0.39 -2.0 855,526 + ABIE41_RS04140 0.39 -0.7 855,526 + ABIE41_RS04140 0.39 +1.0 855,527 - ABIE41_RS04140 0.39 +1.2 855,527 - ABIE41_RS04140 0.39 -1.1 855,527 - ABIE41_RS04140 0.39 +1.5 855,527 - ABIE41_RS04140 0.39 +0.4 855,527 - ABIE41_RS04140 0.39 +0.9 855,527 - ABIE41_RS04140 0.39 -1.8 855,529 - ABIE41_RS04140 0.39 +2.4 855,886 + -0.3 855,886 + -0.3 855,887 - -2.0 855,887 - -0.1 855,887 - -0.8 855,888 + +3.5 855,889 - +0.9 856,023 - +0.3 856,023 - -0.3 856,054 + +0.5 856,055 - +1.5 856,055 - -0.0
Or see this region's nucleotide sequence