Experiment: R2A with 2-pentyl-furan 21.3684 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS04120 and ABIE41_RS04125 are separated by 143 nucleotides ABIE41_RS04125 and ABIE41_RS04130 are separated by 144 nucleotides
ABIE41_RS04120: ABIE41_RS04120 - Crp/Fnr family transcriptional regulator, at 851,244 to 852,002
_RS04120
ABIE41_RS04125: ABIE41_RS04125 - methyltransferase domain-containing protein, at 852,146 to 852,940
_RS04125
ABIE41_RS04130: ABIE41_RS04130 - diguanylate cyclase, at 853,085 to 854,290
_RS04130
Position (kb)
852
853 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 851.191 kb on + strand at 851.191 kb on + strand at 851.191 kb on + strand at 851.191 kb on + strand at 851.191 kb on + strand at 851.191 kb on + strand at 851.192 kb on - strand at 851.192 kb on - strand at 851.192 kb on - strand at 851.192 kb on - strand at 851.192 kb on - strand at 851.192 kb on - strand at 851.259 kb on + strand at 851.433 kb on + strand, within ABIE41_RS04120 at 851.434 kb on - strand, within ABIE41_RS04120 at 851.434 kb on - strand, within ABIE41_RS04120 at 851.434 kb on - strand, within ABIE41_RS04120 at 851.434 kb on - strand, within ABIE41_RS04120 at 851.650 kb on - strand, within ABIE41_RS04120 at 851.650 kb on - strand, within ABIE41_RS04120 at 851.650 kb on - strand, within ABIE41_RS04120 at 851.967 kb on + strand at 851.968 kb on - strand at 852.081 kb on + strand at 852.082 kb on - strand at 852.094 kb on + strand at 853.030 kb on + strand at 853.106 kb on + strand at 853.219 kb on + strand, within ABIE41_RS04130 at 853.394 kb on + strand, within ABIE41_RS04130 at 853.394 kb on + strand, within ABIE41_RS04130 at 853.394 kb on + strand, within ABIE41_RS04130 at 853.395 kb on - strand, within ABIE41_RS04130 at 853.395 kb on - strand, within ABIE41_RS04130 at 853.395 kb on - strand, within ABIE41_RS04130 at 853.395 kb on - strand, within ABIE41_RS04130 at 853.434 kb on - strand, within ABIE41_RS04130 at 853.649 kb on + strand, within ABIE41_RS04130 at 853.649 kb on + strand, within ABIE41_RS04130 at 853.649 kb on + strand, within ABIE41_RS04130 at 853.650 kb on - strand, within ABIE41_RS04130 at 853.650 kb on - strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.655 kb on + strand, within ABIE41_RS04130 at 853.656 kb on - strand, within ABIE41_RS04130 at 853.656 kb on - strand, within ABIE41_RS04130 at 853.656 kb on - strand, within ABIE41_RS04130 at 853.656 kb on - strand, within ABIE41_RS04130 at 853.656 kb on - strand, within ABIE41_RS04130 at 853.656 kb on - strand, within ABIE41_RS04130 at 853.811 kb on + strand, within ABIE41_RS04130 at 853.811 kb on + strand, within ABIE41_RS04130 at 853.841 kb on + strand, within ABIE41_RS04130 at 853.841 kb on + strand, within ABIE41_RS04130 at 853.841 kb on + strand, within ABIE41_RS04130 at 853.841 kb on + strand, within ABIE41_RS04130 at 853.842 kb on - strand, within ABIE41_RS04130 at 853.842 kb on - strand, within ABIE41_RS04130
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 2-pentyl-furan 21.3684 mM remove 851,191 + +2.4 851,191 + +0.1 851,191 + +0.9 851,191 + +0.2 851,191 + -2.2 851,191 + +1.5 851,192 - -0.8 851,192 - -0.3 851,192 - +0.2 851,192 - -2.9 851,192 - -2.8 851,192 - -0.3 851,259 + +0.0 851,433 + ABIE41_RS04120 0.25 -1.3 851,434 - ABIE41_RS04120 0.25 +5.9 851,434 - ABIE41_RS04120 0.25 -0.3 851,434 - ABIE41_RS04120 0.25 -1.4 851,434 - ABIE41_RS04120 0.25 +0.5 851,650 - ABIE41_RS04120 0.53 +0.2 851,650 - ABIE41_RS04120 0.53 +0.1 851,650 - ABIE41_RS04120 0.53 -1.1 851,967 + +0.2 851,968 - +0.6 852,081 + -2.1 852,082 - -1.3 852,094 + -0.0 853,030 + +1.5 853,106 + -0.8 853,219 + ABIE41_RS04130 0.11 +0.4 853,394 + ABIE41_RS04130 0.26 -0.9 853,394 + ABIE41_RS04130 0.26 -1.8 853,394 + ABIE41_RS04130 0.26 +0.6 853,395 - ABIE41_RS04130 0.26 +2.7 853,395 - ABIE41_RS04130 0.26 +0.4 853,395 - ABIE41_RS04130 0.26 +0.7 853,395 - ABIE41_RS04130 0.26 +1.3 853,434 - ABIE41_RS04130 0.29 -0.1 853,649 + ABIE41_RS04130 0.47 -0.9 853,649 + ABIE41_RS04130 0.47 -0.4 853,649 + ABIE41_RS04130 0.47 +0.2 853,650 - ABIE41_RS04130 0.47 -0.2 853,650 - ABIE41_RS04130 0.47 -0.2 853,655 + ABIE41_RS04130 0.47 -0.3 853,655 + ABIE41_RS04130 0.47 -1.3 853,655 + ABIE41_RS04130 0.47 +0.4 853,655 + ABIE41_RS04130 0.47 -0.4 853,655 + ABIE41_RS04130 0.47 -1.1 853,655 + ABIE41_RS04130 0.47 +0.3 853,655 + ABIE41_RS04130 0.47 -0.3 853,655 + ABIE41_RS04130 0.47 -0.8 853,655 + ABIE41_RS04130 0.47 +2.7 853,655 + ABIE41_RS04130 0.47 +0.5 853,655 + ABIE41_RS04130 0.47 +1.0 853,656 - ABIE41_RS04130 0.47 -2.3 853,656 - ABIE41_RS04130 0.47 -0.9 853,656 - ABIE41_RS04130 0.47 -2.5 853,656 - ABIE41_RS04130 0.47 -0.6 853,656 - ABIE41_RS04130 0.47 -2.1 853,656 - ABIE41_RS04130 0.47 -1.7 853,811 + ABIE41_RS04130 0.60 -0.3 853,811 + ABIE41_RS04130 0.60 -0.4 853,841 + ABIE41_RS04130 0.63 +1.3 853,841 + ABIE41_RS04130 0.63 -0.2 853,841 + ABIE41_RS04130 0.63 -1.0 853,841 + ABIE41_RS04130 0.63 -2.3 853,842 - ABIE41_RS04130 0.63 -0.6 853,842 - ABIE41_RS04130 0.63 -0.6
Or see this region's nucleotide sequence