Experiment: R2A with 2-pentyl-furan 21.3684 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS11970 and ABIE41_RS11975 are separated by 243 nucleotides ABIE41_RS11975 and ABIE41_RS11980 are separated by 152 nucleotides ABIE41_RS11980 and ABIE41_RS11985 are separated by 61 nucleotides
ABIE41_RS11970: ABIE41_RS11970 - prephenate dehydrogenase, at 2,446,098 to 2,446,967
_RS11970
ABIE41_RS11975: ABIE41_RS11975 - glutamine-hydrolyzing carbamoyl-phosphate synthase small subunit, at 2,447,211 to 2,448,419
_RS11975
ABIE41_RS11980: ABIE41_RS11980 - GatB/YqeY domain-containing protein, at 2,448,572 to 2,449,033
_RS11980
ABIE41_RS11985: ABIE41_RS11985 - hypothetical protein, at 2,449,095 to 2,449,592
_RS11985
Position (kb)
2447
2448
2449 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2447.043 kb on + strand at 2447.044 kb on - strand at 2447.044 kb on - strand at 2447.044 kb on - strand at 2447.076 kb on + strand at 2447.076 kb on + strand at 2447.077 kb on - strand at 2447.079 kb on + strand at 2447.079 kb on + strand at 2447.079 kb on + strand at 2447.079 kb on + strand at 2447.080 kb on - strand at 2448.424 kb on - strand at 2448.424 kb on - strand at 2448.424 kb on - strand at 2448.785 kb on + strand, within ABIE41_RS11980 at 2448.785 kb on + strand, within ABIE41_RS11980 at 2448.786 kb on - strand, within ABIE41_RS11980 at 2448.786 kb on - strand, within ABIE41_RS11980 at 2448.851 kb on + strand, within ABIE41_RS11980 at 2448.851 kb on + strand, within ABIE41_RS11980 at 2448.852 kb on - strand, within ABIE41_RS11980 at 2448.852 kb on - strand, within ABIE41_RS11980 at 2448.852 kb on - strand, within ABIE41_RS11980 at 2448.852 kb on - strand, within ABIE41_RS11980 at 2448.852 kb on - strand, within ABIE41_RS11980 at 2449.209 kb on + strand, within ABIE41_RS11985 at 2449.249 kb on - strand, within ABIE41_RS11985 at 2449.302 kb on + strand, within ABIE41_RS11985
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 2-pentyl-furan 21.3684 mM remove 2,447,043 + +1.1 2,447,044 - +0.6 2,447,044 - +2.5 2,447,044 - +1.4 2,447,076 + +0.0 2,447,076 + -1.3 2,447,077 - -0.6 2,447,079 + +0.4 2,447,079 + -0.6 2,447,079 + +0.2 2,447,079 + +0.3 2,447,080 - +0.7 2,448,424 - +1.3 2,448,424 - +3.1 2,448,424 - +1.0 2,448,785 + ABIE41_RS11980 0.46 -0.7 2,448,785 + ABIE41_RS11980 0.46 +1.1 2,448,786 - ABIE41_RS11980 0.46 -2.7 2,448,786 - ABIE41_RS11980 0.46 -0.3 2,448,851 + ABIE41_RS11980 0.60 -3.2 2,448,851 + ABIE41_RS11980 0.60 -0.6 2,448,852 - ABIE41_RS11980 0.61 -2.6 2,448,852 - ABIE41_RS11980 0.61 +0.1 2,448,852 - ABIE41_RS11980 0.61 -0.3 2,448,852 - ABIE41_RS11980 0.61 -0.8 2,448,852 - ABIE41_RS11980 0.61 -1.5 2,449,209 + ABIE41_RS11985 0.23 +0.1 2,449,249 - ABIE41_RS11985 0.31 -1.6 2,449,302 + ABIE41_RS11985 0.42 +1.1
Or see this region's nucleotide sequence