Experiment: R2A with 2-pentyl-furan 21.3684 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS10895 and ABIE41_RS10900 are separated by 15 nucleotides ABIE41_RS10900 and ABIE41_RS10905 are separated by 149 nucleotides ABIE41_RS10905 and ABIE41_RS10910 are separated by 94 nucleotides ABIE41_RS10910 and ABIE41_RS10915 are separated by 261 nucleotides
ABIE41_RS10895: ABIE41_RS10895 - sigma-54 dependent transcriptional regulator, at 2,237,602 to 2,238,966
_RS10895
ABIE41_RS10900: ABIE41_RS10900 - hypothetical protein, at 2,238,982 to 2,239,311
_RS10900
ABIE41_RS10905: ABIE41_RS10905 - MFS transporter, at 2,239,461 to 2,240,912
_RS10905
ABIE41_RS10910: ABIE41_RS10910 - hypothetical protein, at 2,241,007 to 2,241,321
_RS10910
ABIE41_RS10915: ABIE41_RS10915 - flagellar biosynthesis protein FlhA, at 2,241,583 to 2,243,664
_RS10915
Position (kb)
2239
2240
2241 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2238.523 kb on + strand, within ABIE41_RS10895 at 2238.524 kb on - strand, within ABIE41_RS10895 at 2238.524 kb on - strand, within ABIE41_RS10895 at 2238.524 kb on - strand, within ABIE41_RS10895 at 2238.524 kb on - strand, within ABIE41_RS10895 at 2238.784 kb on - strand, within ABIE41_RS10895 at 2238.907 kb on + strand at 2238.907 kb on + strand at 2238.907 kb on + strand at 2238.907 kb on + strand at 2238.907 kb on + strand at 2238.908 kb on - strand at 2238.908 kb on - strand at 2238.908 kb on - strand at 2238.999 kb on - strand at 2239.001 kb on + strand at 2239.016 kb on + strand, within ABIE41_RS10900 at 2239.017 kb on - strand, within ABIE41_RS10900 at 2239.017 kb on - strand, within ABIE41_RS10900 at 2239.019 kb on + strand, within ABIE41_RS10900 at 2239.020 kb on - strand, within ABIE41_RS10900 at 2239.034 kb on + strand, within ABIE41_RS10900 at 2239.034 kb on + strand, within ABIE41_RS10900 at 2239.034 kb on + strand, within ABIE41_RS10900 at 2239.034 kb on + strand, within ABIE41_RS10900 at 2239.034 kb on + strand, within ABIE41_RS10900 at 2239.034 kb on + strand, within ABIE41_RS10900 at 2239.034 kb on + strand, within ABIE41_RS10900 at 2239.035 kb on - strand, within ABIE41_RS10900 at 2239.035 kb on - strand, within ABIE41_RS10900 at 2239.037 kb on + strand, within ABIE41_RS10900 at 2239.038 kb on - strand, within ABIE41_RS10900 at 2239.038 kb on - strand, within ABIE41_RS10900 at 2239.052 kb on + strand, within ABIE41_RS10900 at 2239.055 kb on + strand, within ABIE41_RS10900 at 2239.067 kb on + strand, within ABIE41_RS10900 at 2239.068 kb on - strand, within ABIE41_RS10900 at 2239.070 kb on + strand, within ABIE41_RS10900 at 2239.070 kb on + strand, within ABIE41_RS10900 at 2239.070 kb on + strand, within ABIE41_RS10900 at 2239.070 kb on + strand, within ABIE41_RS10900 at 2239.070 kb on + strand, within ABIE41_RS10900 at 2239.070 kb on + strand, within ABIE41_RS10900 at 2239.071 kb on - strand, within ABIE41_RS10900 at 2239.071 kb on - strand, within ABIE41_RS10900 at 2239.071 kb on - strand, within ABIE41_RS10900 at 2239.071 kb on - strand, within ABIE41_RS10900 at 2239.088 kb on + strand, within ABIE41_RS10900 at 2239.089 kb on - strand, within ABIE41_RS10900 at 2239.091 kb on + strand, within ABIE41_RS10900 at 2239.092 kb on - strand, within ABIE41_RS10900 at 2239.112 kb on + strand, within ABIE41_RS10900 at 2239.112 kb on + strand, within ABIE41_RS10900 at 2239.112 kb on + strand, within ABIE41_RS10900 at 2239.112 kb on + strand, within ABIE41_RS10900 at 2239.112 kb on + strand, within ABIE41_RS10900 at 2239.113 kb on - strand, within ABIE41_RS10900 at 2239.113 kb on - strand, within ABIE41_RS10900 at 2239.113 kb on - strand, within ABIE41_RS10900 at 2239.113 kb on - strand, within ABIE41_RS10900 at 2239.113 kb on - strand, within ABIE41_RS10900 at 2239.113 kb on - strand, within ABIE41_RS10900 at 2239.113 kb on - strand, within ABIE41_RS10900 at 2239.113 kb on - strand, within ABIE41_RS10900 at 2239.115 kb on + strand, within ABIE41_RS10900 at 2239.115 kb on + strand, within ABIE41_RS10900 at 2239.115 kb on + strand, within ABIE41_RS10900 at 2239.115 kb on + strand, within ABIE41_RS10900 at 2239.116 kb on - strand, within ABIE41_RS10900 at 2239.116 kb on - strand, within ABIE41_RS10900 at 2239.127 kb on + strand, within ABIE41_RS10900 at 2239.128 kb on - strand, within ABIE41_RS10900 at 2239.356 kb on + strand at 2239.356 kb on + strand at 2239.356 kb on + strand at 2239.356 kb on + strand at 2239.456 kb on + strand at 2239.457 kb on - strand at 2239.457 kb on - strand at 2239.669 kb on + strand, within ABIE41_RS10905 at 2239.670 kb on - strand, within ABIE41_RS10905 at 2239.670 kb on - strand, within ABIE41_RS10905 at 2239.670 kb on - strand, within ABIE41_RS10905 at 2239.670 kb on - strand, within ABIE41_RS10905 at 2239.670 kb on - strand, within ABIE41_RS10905 at 2239.670 kb on - strand, within ABIE41_RS10905 at 2239.670 kb on - strand, within ABIE41_RS10905 at 2239.670 kb on - strand, within ABIE41_RS10905 at 2239.729 kb on + strand, within ABIE41_RS10905 at 2239.822 kb on + strand, within ABIE41_RS10905 at 2239.822 kb on + strand, within ABIE41_RS10905 at 2239.822 kb on + strand, within ABIE41_RS10905 at 2239.823 kb on - strand, within ABIE41_RS10905 at 2239.858 kb on + strand, within ABIE41_RS10905 at 2239.858 kb on + strand, within ABIE41_RS10905 at 2239.859 kb on - strand, within ABIE41_RS10905 at 2239.984 kb on + strand, within ABIE41_RS10905 at 2239.984 kb on + strand, within ABIE41_RS10905 at 2239.984 kb on + strand, within ABIE41_RS10905 at 2239.984 kb on + strand, within ABIE41_RS10905 at 2239.984 kb on + strand, within ABIE41_RS10905 at 2239.984 kb on + strand, within ABIE41_RS10905 at 2239.984 kb on + strand, within ABIE41_RS10905 at 2239.985 kb on - strand, within ABIE41_RS10905 at 2239.985 kb on - strand, within ABIE41_RS10905 at 2239.985 kb on - strand, within ABIE41_RS10905 at 2240.009 kb on - strand, within ABIE41_RS10905 at 2240.009 kb on - strand, within ABIE41_RS10905 at 2240.009 kb on - strand, within ABIE41_RS10905 at 2240.009 kb on - strand, within ABIE41_RS10905 at 2240.029 kb on + strand, within ABIE41_RS10905 at 2240.029 kb on + strand, within ABIE41_RS10905 at 2240.029 kb on + strand, within ABIE41_RS10905 at 2240.030 kb on - strand, within ABIE41_RS10905 at 2240.030 kb on - strand, within ABIE41_RS10905 at 2240.030 kb on - strand, within ABIE41_RS10905 at 2240.172 kb on + strand, within ABIE41_RS10905 at 2240.173 kb on + strand, within ABIE41_RS10905 at 2240.173 kb on + strand, within ABIE41_RS10905 at 2240.173 kb on + strand, within ABIE41_RS10905 at 2240.173 kb on + strand, within ABIE41_RS10905 at 2240.173 kb on + strand, within ABIE41_RS10905 at 2240.174 kb on - strand, within ABIE41_RS10905 at 2240.174 kb on - strand, within ABIE41_RS10905 at 2240.174 kb on - strand, within ABIE41_RS10905 at 2240.174 kb on - strand, within ABIE41_RS10905 at 2240.174 kb on - strand, within ABIE41_RS10905 at 2240.174 kb on - strand, within ABIE41_RS10905 at 2240.174 kb on - strand, within ABIE41_RS10905 at 2240.174 kb on - strand, within ABIE41_RS10905 at 2240.488 kb on + strand, within ABIE41_RS10905 at 2240.489 kb on - strand, within ABIE41_RS10905 at 2240.489 kb on - strand, within ABIE41_RS10905 at 2240.489 kb on - strand, within ABIE41_RS10905 at 2240.489 kb on - strand, within ABIE41_RS10905 at 2240.581 kb on + strand, within ABIE41_RS10905 at 2240.581 kb on + strand, within ABIE41_RS10905 at 2240.581 kb on + strand, within ABIE41_RS10905 at 2240.582 kb on - strand, within ABIE41_RS10905 at 2240.674 kb on + strand, within ABIE41_RS10905 at 2240.674 kb on + strand, within ABIE41_RS10905 at 2240.674 kb on + strand, within ABIE41_RS10905 at 2240.674 kb on + strand, within ABIE41_RS10905 at 2240.675 kb on - strand, within ABIE41_RS10905 at 2240.816 kb on + strand at 2240.817 kb on - strand at 2240.817 kb on - strand at 2241.057 kb on - strand, within ABIE41_RS10910 at 2241.057 kb on - strand, within ABIE41_RS10910 at 2241.059 kb on + strand, within ABIE41_RS10910 at 2241.059 kb on + strand, within ABIE41_RS10910 at 2241.059 kb on + strand, within ABIE41_RS10910 at 2241.059 kb on + strand, within ABIE41_RS10910 at 2241.060 kb on - strand, within ABIE41_RS10910 at 2241.336 kb on - strand at 2241.336 kb on - strand at 2241.336 kb on - strand at 2241.351 kb on + strand at 2241.352 kb on - strand at 2241.412 kb on + strand at 2241.412 kb on + strand at 2241.452 kb on + strand at 2241.453 kb on - strand at 2241.466 kb on + strand at 2241.467 kb on - strand at 2241.483 kb on + strand at 2241.484 kb on - strand at 2241.484 kb on - strand at 2241.484 kb on - strand at 2241.552 kb on - strand at 2241.552 kb on - strand at 2241.552 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 2-pentyl-furan 21.3684 mM remove 2,238,523 + ABIE41_RS10895 0.67 +1.1 2,238,524 - ABIE41_RS10895 0.68 +0.7 2,238,524 - ABIE41_RS10895 0.68 -0.1 2,238,524 - ABIE41_RS10895 0.68 -0.6 2,238,524 - ABIE41_RS10895 0.68 -0.4 2,238,784 - ABIE41_RS10895 0.87 -1.2 2,238,907 + -1.1 2,238,907 + +0.5 2,238,907 + -0.6 2,238,907 + -1.9 2,238,907 + +1.9 2,238,908 - +0.3 2,238,908 - +1.9 2,238,908 - -1.3 2,238,999 - -0.1 2,239,001 + -0.7 2,239,016 + ABIE41_RS10900 0.10 +1.1 2,239,017 - ABIE41_RS10900 0.11 +0.2 2,239,017 - ABIE41_RS10900 0.11 -1.7 2,239,019 + ABIE41_RS10900 0.11 +0.8 2,239,020 - ABIE41_RS10900 0.12 -0.2 2,239,034 + ABIE41_RS10900 0.16 -2.3 2,239,034 + ABIE41_RS10900 0.16 -0.5 2,239,034 + ABIE41_RS10900 0.16 -0.2 2,239,034 + ABIE41_RS10900 0.16 +0.6 2,239,034 + ABIE41_RS10900 0.16 -1.8 2,239,034 + ABIE41_RS10900 0.16 -1.3 2,239,034 + ABIE41_RS10900 0.16 +2.7 2,239,035 - ABIE41_RS10900 0.16 +0.3 2,239,035 - ABIE41_RS10900 0.16 -0.5 2,239,037 + ABIE41_RS10900 0.17 -0.5 2,239,038 - ABIE41_RS10900 0.17 -0.2 2,239,038 - ABIE41_RS10900 0.17 -2.2 2,239,052 + ABIE41_RS10900 0.21 -0.5 2,239,055 + ABIE41_RS10900 0.22 -0.4 2,239,067 + ABIE41_RS10900 0.26 +0.7 2,239,068 - ABIE41_RS10900 0.26 -0.8 2,239,070 + ABIE41_RS10900 0.27 +0.3 2,239,070 + ABIE41_RS10900 0.27 -2.5 2,239,070 + ABIE41_RS10900 0.27 -2.6 2,239,070 + ABIE41_RS10900 0.27 +0.1 2,239,070 + ABIE41_RS10900 0.27 +0.2 2,239,070 + ABIE41_RS10900 0.27 -0.6 2,239,071 - ABIE41_RS10900 0.27 +1.1 2,239,071 - ABIE41_RS10900 0.27 -0.1 2,239,071 - ABIE41_RS10900 0.27 +0.3 2,239,071 - ABIE41_RS10900 0.27 -1.0 2,239,088 + ABIE41_RS10900 0.32 -1.1 2,239,089 - ABIE41_RS10900 0.32 +0.1 2,239,091 + ABIE41_RS10900 0.33 -1.3 2,239,092 - ABIE41_RS10900 0.33 -0.7 2,239,112 + ABIE41_RS10900 0.39 +0.3 2,239,112 + ABIE41_RS10900 0.39 -0.7 2,239,112 + ABIE41_RS10900 0.39 +0.8 2,239,112 + ABIE41_RS10900 0.39 +2.2 2,239,112 + ABIE41_RS10900 0.39 +2.3 2,239,113 - ABIE41_RS10900 0.40 -0.2 2,239,113 - ABIE41_RS10900 0.40 +0.4 2,239,113 - ABIE41_RS10900 0.40 -0.3 2,239,113 - ABIE41_RS10900 0.40 -2.4 2,239,113 - ABIE41_RS10900 0.40 -0.6 2,239,113 - ABIE41_RS10900 0.40 +0.5 2,239,113 - ABIE41_RS10900 0.40 +0.9 2,239,113 - ABIE41_RS10900 0.40 +0.1 2,239,115 + ABIE41_RS10900 0.40 +1.2 2,239,115 + ABIE41_RS10900 0.40 +0.5 2,239,115 + ABIE41_RS10900 0.40 +0.3 2,239,115 + ABIE41_RS10900 0.40 +0.2 2,239,116 - ABIE41_RS10900 0.41 +0.0 2,239,116 - ABIE41_RS10900 0.41 +1.3 2,239,127 + ABIE41_RS10900 0.44 +0.9 2,239,128 - ABIE41_RS10900 0.44 -0.3 2,239,356 + +0.1 2,239,356 + -1.2 2,239,356 + -2.2 2,239,356 + +0.2 2,239,456 + +1.5 2,239,457 - -1.6 2,239,457 - +1.3 2,239,669 + ABIE41_RS10905 0.14 -0.8 2,239,670 - ABIE41_RS10905 0.14 +0.3 2,239,670 - ABIE41_RS10905 0.14 +0.8 2,239,670 - ABIE41_RS10905 0.14 +0.2 2,239,670 - ABIE41_RS10905 0.14 -0.3 2,239,670 - ABIE41_RS10905 0.14 +0.1 2,239,670 - ABIE41_RS10905 0.14 -0.4 2,239,670 - ABIE41_RS10905 0.14 +0.5 2,239,670 - ABIE41_RS10905 0.14 +0.1 2,239,729 + ABIE41_RS10905 0.18 -2.4 2,239,822 + ABIE41_RS10905 0.25 +1.8 2,239,822 + ABIE41_RS10905 0.25 -1.6 2,239,822 + ABIE41_RS10905 0.25 -4.1 2,239,823 - ABIE41_RS10905 0.25 -0.7 2,239,858 + ABIE41_RS10905 0.27 -0.2 2,239,858 + ABIE41_RS10905 0.27 -0.9 2,239,859 - ABIE41_RS10905 0.27 -1.4 2,239,984 + ABIE41_RS10905 0.36 -0.1 2,239,984 + ABIE41_RS10905 0.36 +1.1 2,239,984 + ABIE41_RS10905 0.36 +1.4 2,239,984 + ABIE41_RS10905 0.36 +1.1 2,239,984 + ABIE41_RS10905 0.36 +0.2 2,239,984 + ABIE41_RS10905 0.36 -1.3 2,239,984 + ABIE41_RS10905 0.36 +0.7 2,239,985 - ABIE41_RS10905 0.36 -2.4 2,239,985 - ABIE41_RS10905 0.36 -1.0 2,239,985 - ABIE41_RS10905 0.36 -0.5 2,240,009 - ABIE41_RS10905 0.38 +0.2 2,240,009 - ABIE41_RS10905 0.38 +0.5 2,240,009 - ABIE41_RS10905 0.38 -1.8 2,240,009 - ABIE41_RS10905 0.38 -2.2 2,240,029 + ABIE41_RS10905 0.39 +0.8 2,240,029 + ABIE41_RS10905 0.39 +1.4 2,240,029 + ABIE41_RS10905 0.39 +0.7 2,240,030 - ABIE41_RS10905 0.39 +0.9 2,240,030 - ABIE41_RS10905 0.39 +0.7 2,240,030 - ABIE41_RS10905 0.39 -0.2 2,240,172 + ABIE41_RS10905 0.49 -2.5 2,240,173 + ABIE41_RS10905 0.49 +0.4 2,240,173 + ABIE41_RS10905 0.49 +0.6 2,240,173 + ABIE41_RS10905 0.49 +0.9 2,240,173 + ABIE41_RS10905 0.49 +1.9 2,240,173 + ABIE41_RS10905 0.49 -0.3 2,240,174 - ABIE41_RS10905 0.49 +0.9 2,240,174 - ABIE41_RS10905 0.49 -0.3 2,240,174 - ABIE41_RS10905 0.49 +3.7 2,240,174 - ABIE41_RS10905 0.49 +1.4 2,240,174 - ABIE41_RS10905 0.49 -2.9 2,240,174 - ABIE41_RS10905 0.49 -1.7 2,240,174 - ABIE41_RS10905 0.49 -3.0 2,240,174 - ABIE41_RS10905 0.49 -0.6 2,240,488 + ABIE41_RS10905 0.71 +0.4 2,240,489 - ABIE41_RS10905 0.71 +0.2 2,240,489 - ABIE41_RS10905 0.71 +0.3 2,240,489 - ABIE41_RS10905 0.71 -0.0 2,240,489 - ABIE41_RS10905 0.71 -0.7 2,240,581 + ABIE41_RS10905 0.77 +2.5 2,240,581 + ABIE41_RS10905 0.77 +1.2 2,240,581 + ABIE41_RS10905 0.77 -1.0 2,240,582 - ABIE41_RS10905 0.77 +1.1 2,240,674 + ABIE41_RS10905 0.84 +1.5 2,240,674 + ABIE41_RS10905 0.84 +0.7 2,240,674 + ABIE41_RS10905 0.84 -0.2 2,240,674 + ABIE41_RS10905 0.84 +0.9 2,240,675 - ABIE41_RS10905 0.84 +0.0 2,240,816 + +0.1 2,240,817 - -0.3 2,240,817 - +0.8 2,241,057 - ABIE41_RS10910 0.16 -0.3 2,241,057 - ABIE41_RS10910 0.16 -1.3 2,241,059 + ABIE41_RS10910 0.17 +0.4 2,241,059 + ABIE41_RS10910 0.17 -0.4 2,241,059 + ABIE41_RS10910 0.17 +1.0 2,241,059 + ABIE41_RS10910 0.17 +1.8 2,241,060 - ABIE41_RS10910 0.17 -0.6 2,241,336 - -3.2 2,241,336 - -1.8 2,241,336 - -2.0 2,241,351 + -0.4 2,241,352 - -2.6 2,241,412 + -1.0 2,241,412 + -0.4 2,241,452 + -2.0 2,241,453 - -2.5 2,241,466 + +1.4 2,241,467 - +0.4 2,241,483 + +2.6 2,241,484 - +2.1 2,241,484 - -0.6 2,241,484 - +1.1 2,241,552 - -1.7 2,241,552 - -4.1 2,241,552 - -0.5
Or see this region's nucleotide sequence