Experiment: R2A with 2-pentyl-furan 21.3684 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS07535 and ABIE41_RS07540 are separated by 130 nucleotides ABIE41_RS07540 and ABIE41_RS07545 are separated by 27 nucleotides ABIE41_RS07545 and ABIE41_RS07550 overlap by 4 nucleotides
ABIE41_RS07535: ABIE41_RS07535 - glucose-6-phosphate isomerase, at 1,544,746 to 1,546,362
_RS07535
ABIE41_RS07540: ABIE41_RS07540 - transporter substrate-binding domain-containing protein, at 1,546,493 to 1,547,248
_RS07540
ABIE41_RS07545: ABIE41_RS07545 - RNA pyrophosphohydrolase, at 1,547,276 to 1,547,788
_RS07545
ABIE41_RS07550: ABIE41_RS07550 - divergent polysaccharide deacetylase family protein, at 1,547,785 to 1,548,999
_RS07550
Position (kb)
1546
1547
1548 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1545.855 kb on - strand, within ABIE41_RS07535 at 1545.878 kb on + strand, within ABIE41_RS07535 at 1546.827 kb on + strand, within ABIE41_RS07540 at 1546.827 kb on + strand, within ABIE41_RS07540 at 1546.828 kb on - strand, within ABIE41_RS07540 at 1546.828 kb on - strand, within ABIE41_RS07540 at 1546.914 kb on + strand, within ABIE41_RS07540 at 1546.914 kb on + strand, within ABIE41_RS07540 at 1546.914 kb on + strand, within ABIE41_RS07540 at 1546.914 kb on + strand, within ABIE41_RS07540 at 1546.914 kb on + strand, within ABIE41_RS07540 at 1546.915 kb on - strand, within ABIE41_RS07540 at 1546.915 kb on - strand, within ABIE41_RS07540 at 1546.915 kb on - strand, within ABIE41_RS07540 at 1546.915 kb on - strand, within ABIE41_RS07540 at 1546.935 kb on + strand, within ABIE41_RS07540 at 1547.043 kb on + strand, within ABIE41_RS07540 at 1547.044 kb on - strand, within ABIE41_RS07540 at 1547.044 kb on - strand, within ABIE41_RS07540 at 1547.044 kb on - strand, within ABIE41_RS07540 at 1547.319 kb on + strand at 1547.760 kb on + strand at 1548.191 kb on + strand, within ABIE41_RS07550 at 1548.192 kb on - strand, within ABIE41_RS07550
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 2-pentyl-furan 21.3684 mM remove 1,545,855 - ABIE41_RS07535 0.69 -1.8 1,545,878 + ABIE41_RS07535 0.70 -0.2 1,546,827 + ABIE41_RS07540 0.44 +0.6 1,546,827 + ABIE41_RS07540 0.44 -0.0 1,546,828 - ABIE41_RS07540 0.44 +0.4 1,546,828 - ABIE41_RS07540 0.44 +0.5 1,546,914 + ABIE41_RS07540 0.56 +0.6 1,546,914 + ABIE41_RS07540 0.56 +1.5 1,546,914 + ABIE41_RS07540 0.56 +0.5 1,546,914 + ABIE41_RS07540 0.56 -3.2 1,546,914 + ABIE41_RS07540 0.56 -0.1 1,546,915 - ABIE41_RS07540 0.56 +0.6 1,546,915 - ABIE41_RS07540 0.56 +1.2 1,546,915 - ABIE41_RS07540 0.56 +1.9 1,546,915 - ABIE41_RS07540 0.56 -0.9 1,546,935 + ABIE41_RS07540 0.58 -1.1 1,547,043 + ABIE41_RS07540 0.73 -0.4 1,547,044 - ABIE41_RS07540 0.73 +0.2 1,547,044 - ABIE41_RS07540 0.73 -2.0 1,547,044 - ABIE41_RS07540 0.73 +0.4 1,547,319 + -0.5 1,547,760 + -1.6 1,548,191 + ABIE41_RS07550 0.33 +0.6 1,548,192 - ABIE41_RS07550 0.33 -0.7
Or see this region's nucleotide sequence