Experiment: R2A with 2-pentyl-furan 21.3684 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS00125 and ABIE41_RS00130 are separated by 220 nucleotides ABIE41_RS00130 and ABIE41_RS00135 are separated by 158 nucleotides ABIE41_RS00135 and ABIE41_RS00140 are separated by 2 nucleotides
ABIE41_RS00125: ABIE41_RS00125 - RNA polymerase factor sigma-54, at 18,900 to 20,426
_RS00125
ABIE41_RS00130: ABIE41_RS00130 - LPS export ABC transporter ATP-binding protein, at 20,647 to 21,435
_RS00130
ABIE41_RS00135: ABIE41_RS00135 - LptA/OstA family protein, at 21,594 to 22,271
_RS00135
ABIE41_RS00140: ABIE41_RS00140 - LPS export ABC transporter periplasmic protein LptC, at 22,274 to 23,050
_RS00140
Position (kb) 20
21
22 Strain fitness (log2 ratio) -2
-1
0
1 at 19.814 kb on + strand, within ABIE41_RS00125 at 19.815 kb on - strand, within ABIE41_RS00125 at 20.060 kb on - strand, within ABIE41_RS00125 at 20.060 kb on - strand, within ABIE41_RS00125 at 20.060 kb on - strand, within ABIE41_RS00125 at 20.206 kb on + strand, within ABIE41_RS00125 at 20.207 kb on - strand, within ABIE41_RS00125 at 20.207 kb on - strand, within ABIE41_RS00125 at 20.207 kb on - strand, within ABIE41_RS00125 at 20.545 kb on + strand at 20.545 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 2-pentyl-furan 21.3684 mM remove 19,814 + ABIE41_RS00125 0.60 -2.1 19,815 - ABIE41_RS00125 0.60 +1.7 20,060 - ABIE41_RS00125 0.76 -0.3 20,060 - ABIE41_RS00125 0.76 +1.0 20,060 - ABIE41_RS00125 0.76 -0.7 20,206 + ABIE41_RS00125 0.86 -1.0 20,207 - ABIE41_RS00125 0.86 -0.4 20,207 - ABIE41_RS00125 0.86 -1.1 20,207 - ABIE41_RS00125 0.86 -1.8 20,545 + -2.0 20,545 + -0.3
Or see this region's nucleotide sequence