Experiment: R2A with 2-pentyl-furan 21.3684 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS12985 and ABIE41_RS12990 are separated by 45 nucleotides ABIE41_RS12990 and ABIE41_RS12995 are separated by 229 nucleotides ABIE41_RS12995 and ABIE41_RS13000 are separated by 390 nucleotides
ABIE41_RS12985: ABIE41_RS12985 - M3 family metallopeptidase, at 2,655,336 to 2,657,438
_RS12985
ABIE41_RS12990: ABIE41_RS12990 - hypothetical protein, at 2,657,484 to 2,657,825
_RS12990
ABIE41_RS12995: ABIE41_RS12995 - YoaK family protein, at 2,658,055 to 2,658,696
_RS12995
ABIE41_RS13000: ABIE41_RS13000 - 2-isopropylmalate synthase, at 2,659,087 to 2,660,670
_RS13000
Position (kb)
2658
2659 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2657.226 kb on + strand, within ABIE41_RS12985 at 2657.226 kb on + strand, within ABIE41_RS12985 at 2657.226 kb on + strand, within ABIE41_RS12985 at 2657.227 kb on - strand, within ABIE41_RS12985 at 2657.227 kb on - strand, within ABIE41_RS12985 at 2657.227 kb on - strand, within ABIE41_RS12985 at 2657.227 kb on - strand, within ABIE41_RS12985 at 2657.232 kb on + strand at 2657.232 kb on + strand at 2657.232 kb on + strand at 2657.232 kb on + strand at 2657.233 kb on - strand at 2657.329 kb on - strand at 2657.329 kb on - strand at 2657.329 kb on - strand at 2657.329 kb on - strand at 2657.329 kb on - strand at 2657.334 kb on + strand at 2657.436 kb on + strand at 2657.436 kb on + strand at 2657.437 kb on - strand at 2657.437 kb on - strand at 2657.437 kb on - strand at 2657.439 kb on + strand at 2657.440 kb on - strand at 2657.934 kb on - strand at 2658.037 kb on + strand at 2658.037 kb on + strand at 2658.038 kb on - strand at 2658.038 kb on - strand at 2658.038 kb on - strand at 2658.052 kb on + strand at 2658.143 kb on + strand, within ABIE41_RS12995 at 2658.143 kb on + strand, within ABIE41_RS12995 at 2658.144 kb on - strand, within ABIE41_RS12995 at 2658.247 kb on - strand, within ABIE41_RS12995 at 2658.279 kb on + strand, within ABIE41_RS12995 at 2658.280 kb on - strand, within ABIE41_RS12995 at 2658.281 kb on + strand, within ABIE41_RS12995 at 2658.281 kb on + strand, within ABIE41_RS12995 at 2658.281 kb on + strand, within ABIE41_RS12995 at 2658.281 kb on + strand, within ABIE41_RS12995 at 2658.281 kb on + strand, within ABIE41_RS12995 at 2658.281 kb on + strand, within ABIE41_RS12995 at 2658.281 kb on + strand, within ABIE41_RS12995 at 2658.281 kb on + strand, within ABIE41_RS12995 at 2658.281 kb on + strand, within ABIE41_RS12995 at 2658.281 kb on + strand, within ABIE41_RS12995 at 2658.282 kb on - strand, within ABIE41_RS12995 at 2658.282 kb on - strand, within ABIE41_RS12995 at 2658.282 kb on - strand, within ABIE41_RS12995 at 2658.282 kb on - strand, within ABIE41_RS12995 at 2658.282 kb on - strand, within ABIE41_RS12995 at 2658.282 kb on - strand, within ABIE41_RS12995 at 2658.282 kb on - strand, within ABIE41_RS12995 at 2658.282 kb on - strand, within ABIE41_RS12995 at 2658.282 kb on - strand, within ABIE41_RS12995 at 2658.685 kb on - strand at 2658.686 kb on + strand at 2658.686 kb on + strand at 2658.686 kb on + strand at 2658.687 kb on - strand at 2658.687 kb on - strand at 2658.687 kb on - strand at 2658.687 kb on - strand at 2658.797 kb on + strand at 2658.797 kb on + strand at 2658.797 kb on + strand at 2658.797 kb on + strand at 2658.798 kb on - strand at 2658.798 kb on - strand at 2658.798 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 2-pentyl-furan 21.3684 mM remove 2,657,226 + ABIE41_RS12985 0.90 +1.1 2,657,226 + ABIE41_RS12985 0.90 +1.6 2,657,226 + ABIE41_RS12985 0.90 +1.5 2,657,227 - ABIE41_RS12985 0.90 +1.1 2,657,227 - ABIE41_RS12985 0.90 +0.9 2,657,227 - ABIE41_RS12985 0.90 +1.0 2,657,227 - ABIE41_RS12985 0.90 +0.6 2,657,232 + +0.3 2,657,232 + +1.3 2,657,232 + +1.3 2,657,232 + +2.3 2,657,233 - +0.9 2,657,329 - +0.7 2,657,329 - +1.3 2,657,329 - +0.9 2,657,329 - +0.4 2,657,329 - +0.7 2,657,334 + +0.8 2,657,436 + +0.9 2,657,436 + +1.4 2,657,437 - +0.1 2,657,437 - +0.7 2,657,437 - +1.2 2,657,439 + +0.9 2,657,440 - +0.3 2,657,934 - +0.0 2,658,037 + +0.3 2,658,037 + +1.3 2,658,038 - +1.3 2,658,038 - +1.1 2,658,038 - -1.9 2,658,052 + +0.2 2,658,143 + ABIE41_RS12995 0.14 -0.7 2,658,143 + ABIE41_RS12995 0.14 -1.1 2,658,144 - ABIE41_RS12995 0.14 +0.2 2,658,247 - ABIE41_RS12995 0.30 +2.6 2,658,279 + ABIE41_RS12995 0.35 -1.0 2,658,280 - ABIE41_RS12995 0.35 -0.6 2,658,281 + ABIE41_RS12995 0.35 +0.2 2,658,281 + ABIE41_RS12995 0.35 +0.5 2,658,281 + ABIE41_RS12995 0.35 -0.4 2,658,281 + ABIE41_RS12995 0.35 -0.8 2,658,281 + ABIE41_RS12995 0.35 +0.3 2,658,281 + ABIE41_RS12995 0.35 -0.2 2,658,281 + ABIE41_RS12995 0.35 -0.6 2,658,281 + ABIE41_RS12995 0.35 -0.3 2,658,281 + ABIE41_RS12995 0.35 +1.4 2,658,281 + ABIE41_RS12995 0.35 +0.0 2,658,282 - ABIE41_RS12995 0.35 -1.6 2,658,282 - ABIE41_RS12995 0.35 -1.6 2,658,282 - ABIE41_RS12995 0.35 -0.0 2,658,282 - ABIE41_RS12995 0.35 -0.2 2,658,282 - ABIE41_RS12995 0.35 +0.4 2,658,282 - ABIE41_RS12995 0.35 -0.6 2,658,282 - ABIE41_RS12995 0.35 -0.1 2,658,282 - ABIE41_RS12995 0.35 -0.4 2,658,282 - ABIE41_RS12995 0.35 +0.3 2,658,685 - -0.7 2,658,686 + -1.3 2,658,686 + +0.3 2,658,686 + +3.6 2,658,687 - +0.3 2,658,687 - +0.6 2,658,687 - -1.2 2,658,687 - +0.6 2,658,797 + +0.5 2,658,797 + +0.2 2,658,797 + +0.4 2,658,797 + -0.3 2,658,798 - -0.5 2,658,798 - +2.8 2,658,798 - +0.0
Or see this region's nucleotide sequence