Experiment: R2A with 2-pentyl-furan 21.3684 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS13975 and ABIE41_RS13980 are separated by 237 nucleotides ABIE41_RS13980 and ABIE41_RS13985 overlap by 7 nucleotides ABIE41_RS13985 and ABIE41_RS13990 are separated by 310 nucleotides ABIE41_RS13990 and ABIE41_RS13995 are separated by 74 nucleotides
ABIE41_RS13975: ABIE41_RS13975 - AraC family transcriptional regulator, at 2,869,916 to 2,870,776
_RS13975
ABIE41_RS13980: ABIE41_RS13980 - bacteriochlorophyll 4-vinyl reductase, at 2,871,014 to 2,871,700
_RS13980
ABIE41_RS13985: ABIE41_RS13985 - magnesium-protoporphyrin IX monomethyl ester anaerobic oxidative cyclase, at 2,871,694 to 2,873,349
_RS13985
ABIE41_RS13990: ABIE41_RS13990 - DUF3126 family protein, at 2,873,660 to 2,873,872
_RS13990
ABIE41_RS13995: ABIE41_RS13995 - hypothetical protein, at 2,873,947 to 2,874,702
_RS13995
Position (kb)
2871
2872
2873
2874 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2870.838 kb on + strand at 2870.838 kb on + strand at 2870.838 kb on + strand at 2870.838 kb on + strand at 2870.839 kb on - strand at 2870.839 kb on - strand at 2871.142 kb on - strand, within ABIE41_RS13980 at 2871.405 kb on + strand, within ABIE41_RS13980 at 2871.405 kb on + strand, within ABIE41_RS13980 at 2871.405 kb on + strand, within ABIE41_RS13980 at 2871.405 kb on + strand, within ABIE41_RS13980 at 2871.405 kb on + strand, within ABIE41_RS13980 at 2871.405 kb on + strand, within ABIE41_RS13980 at 2871.405 kb on + strand, within ABIE41_RS13980 at 2871.405 kb on + strand, within ABIE41_RS13980 at 2871.406 kb on - strand, within ABIE41_RS13980 at 2871.406 kb on - strand, within ABIE41_RS13980 at 2871.406 kb on - strand, within ABIE41_RS13980 at 2871.406 kb on - strand, within ABIE41_RS13980 at 2871.406 kb on - strand, within ABIE41_RS13980 at 2871.406 kb on - strand, within ABIE41_RS13980 at 2871.406 kb on - strand, within ABIE41_RS13980 at 2871.744 kb on + strand at 2871.745 kb on - strand at 2871.745 kb on - strand at 2871.781 kb on + strand at 2871.950 kb on + strand, within ABIE41_RS13985 at 2871.951 kb on - strand, within ABIE41_RS13985 at 2872.004 kb on + strand, within ABIE41_RS13985 at 2872.061 kb on + strand, within ABIE41_RS13985 at 2872.073 kb on + strand, within ABIE41_RS13985 at 2872.148 kb on + strand, within ABIE41_RS13985 at 2872.148 kb on + strand, within ABIE41_RS13985 at 2872.148 kb on + strand, within ABIE41_RS13985 at 2872.148 kb on + strand, within ABIE41_RS13985 at 2872.149 kb on - strand, within ABIE41_RS13985 at 2872.220 kb on + strand, within ABIE41_RS13985 at 2872.220 kb on + strand, within ABIE41_RS13985 at 2872.220 kb on + strand, within ABIE41_RS13985 at 2872.220 kb on + strand, within ABIE41_RS13985 at 2872.220 kb on + strand, within ABIE41_RS13985 at 2872.221 kb on - strand, within ABIE41_RS13985 at 2872.221 kb on - strand, within ABIE41_RS13985 at 2872.221 kb on - strand, within ABIE41_RS13985 at 2872.357 kb on + strand, within ABIE41_RS13985 at 2872.358 kb on - strand, within ABIE41_RS13985 at 2872.787 kb on + strand, within ABIE41_RS13985 at 2872.787 kb on + strand, within ABIE41_RS13985 at 2872.787 kb on + strand, within ABIE41_RS13985 at 2872.787 kb on + strand, within ABIE41_RS13985 at 2872.788 kb on - strand, within ABIE41_RS13985 at 2872.788 kb on - strand, within ABIE41_RS13985 at 2872.788 kb on - strand, within ABIE41_RS13985 at 2872.788 kb on - strand, within ABIE41_RS13985 at 2872.788 kb on - strand, within ABIE41_RS13985 at 2872.788 kb on - strand, within ABIE41_RS13985 at 2872.788 kb on - strand, within ABIE41_RS13985 at 2872.788 kb on - strand, within ABIE41_RS13985 at 2872.788 kb on - strand, within ABIE41_RS13985 at 2872.788 kb on - strand, within ABIE41_RS13985 at 2872.788 kb on - strand, within ABIE41_RS13985 at 2872.793 kb on + strand, within ABIE41_RS13985 at 2872.793 kb on + strand, within ABIE41_RS13985 at 2872.793 kb on + strand, within ABIE41_RS13985 at 2872.794 kb on - strand, within ABIE41_RS13985 at 2872.794 kb on - strand, within ABIE41_RS13985 at 2872.794 kb on - strand, within ABIE41_RS13985 at 2873.030 kb on + strand, within ABIE41_RS13985 at 2873.030 kb on + strand, within ABIE41_RS13985 at 2873.030 kb on + strand, within ABIE41_RS13985 at 2873.030 kb on + strand, within ABIE41_RS13985 at 2873.030 kb on + strand, within ABIE41_RS13985 at 2873.030 kb on + strand, within ABIE41_RS13985 at 2873.030 kb on + strand, within ABIE41_RS13985 at 2873.031 kb on - strand, within ABIE41_RS13985 at 2873.117 kb on + strand, within ABIE41_RS13985 at 2873.117 kb on + strand, within ABIE41_RS13985 at 2873.118 kb on - strand, within ABIE41_RS13985 at 2873.118 kb on - strand, within ABIE41_RS13985 at 2873.118 kb on - strand, within ABIE41_RS13985 at 2873.118 kb on - strand, within ABIE41_RS13985 at 2873.118 kb on - strand, within ABIE41_RS13985 at 2873.267 kb on + strand at 2873.267 kb on + strand at 2873.267 kb on + strand at 2873.268 kb on - strand at 2873.321 kb on + strand at 2873.321 kb on + strand at 2873.321 kb on + strand at 2873.321 kb on + strand at 2873.322 kb on - strand at 2873.322 kb on - strand at 2873.322 kb on - strand at 2873.693 kb on + strand, within ABIE41_RS13990 at 2873.693 kb on + strand, within ABIE41_RS13990 at 2873.693 kb on + strand, within ABIE41_RS13990 at 2873.694 kb on - strand, within ABIE41_RS13990 at 2873.694 kb on - strand, within ABIE41_RS13990 at 2873.932 kb on - strand at 2874.161 kb on + strand, within ABIE41_RS13995 at 2874.161 kb on + strand, within ABIE41_RS13995 at 2874.161 kb on + strand, within ABIE41_RS13995 at 2874.161 kb on + strand, within ABIE41_RS13995 at 2874.162 kb on - strand, within ABIE41_RS13995 at 2874.162 kb on - strand, within ABIE41_RS13995 at 2874.162 kb on - strand, within ABIE41_RS13995 at 2874.162 kb on - strand, within ABIE41_RS13995 at 2874.257 kb on + strand, within ABIE41_RS13995 at 2874.258 kb on - strand, within ABIE41_RS13995 at 2874.291 kb on + strand, within ABIE41_RS13995 at 2874.291 kb on + strand, within ABIE41_RS13995
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 2-pentyl-furan 21.3684 mM remove 2,870,838 + +1.3 2,870,838 + -0.2 2,870,838 + +1.9 2,870,838 + +0.3 2,870,839 - +0.0 2,870,839 - +0.2 2,871,142 - ABIE41_RS13980 0.19 +0.9 2,871,405 + ABIE41_RS13980 0.57 -0.0 2,871,405 + ABIE41_RS13980 0.57 -1.1 2,871,405 + ABIE41_RS13980 0.57 -1.0 2,871,405 + ABIE41_RS13980 0.57 +1.1 2,871,405 + ABIE41_RS13980 0.57 -0.5 2,871,405 + ABIE41_RS13980 0.57 +0.5 2,871,405 + ABIE41_RS13980 0.57 -0.3 2,871,405 + ABIE41_RS13980 0.57 -0.2 2,871,406 - ABIE41_RS13980 0.57 +0.0 2,871,406 - ABIE41_RS13980 0.57 -0.3 2,871,406 - ABIE41_RS13980 0.57 -0.4 2,871,406 - ABIE41_RS13980 0.57 -1.5 2,871,406 - ABIE41_RS13980 0.57 +0.3 2,871,406 - ABIE41_RS13980 0.57 -0.6 2,871,406 - ABIE41_RS13980 0.57 -1.2 2,871,744 + -0.4 2,871,745 - -0.5 2,871,745 - -1.5 2,871,781 + +0.7 2,871,950 + ABIE41_RS13985 0.15 -0.5 2,871,951 - ABIE41_RS13985 0.16 -0.1 2,872,004 + ABIE41_RS13985 0.19 -1.8 2,872,061 + ABIE41_RS13985 0.22 -1.1 2,872,073 + ABIE41_RS13985 0.23 +0.9 2,872,148 + ABIE41_RS13985 0.27 -0.2 2,872,148 + ABIE41_RS13985 0.27 +0.6 2,872,148 + ABIE41_RS13985 0.27 -0.4 2,872,148 + ABIE41_RS13985 0.27 +0.4 2,872,149 - ABIE41_RS13985 0.27 +0.5 2,872,220 + ABIE41_RS13985 0.32 -0.8 2,872,220 + ABIE41_RS13985 0.32 +0.3 2,872,220 + ABIE41_RS13985 0.32 -0.1 2,872,220 + ABIE41_RS13985 0.32 -0.3 2,872,220 + ABIE41_RS13985 0.32 +0.8 2,872,221 - ABIE41_RS13985 0.32 +0.1 2,872,221 - ABIE41_RS13985 0.32 +2.7 2,872,221 - ABIE41_RS13985 0.32 +0.2 2,872,357 + ABIE41_RS13985 0.40 -0.7 2,872,358 - ABIE41_RS13985 0.40 -0.5 2,872,787 + ABIE41_RS13985 0.66 +1.2 2,872,787 + ABIE41_RS13985 0.66 +1.5 2,872,787 + ABIE41_RS13985 0.66 +0.5 2,872,787 + ABIE41_RS13985 0.66 -0.7 2,872,788 - ABIE41_RS13985 0.66 -1.7 2,872,788 - ABIE41_RS13985 0.66 -0.5 2,872,788 - ABIE41_RS13985 0.66 +0.2 2,872,788 - ABIE41_RS13985 0.66 +0.3 2,872,788 - ABIE41_RS13985 0.66 -1.3 2,872,788 - ABIE41_RS13985 0.66 +1.3 2,872,788 - ABIE41_RS13985 0.66 -1.3 2,872,788 - ABIE41_RS13985 0.66 -0.1 2,872,788 - ABIE41_RS13985 0.66 +0.1 2,872,788 - ABIE41_RS13985 0.66 -0.7 2,872,788 - ABIE41_RS13985 0.66 -0.1 2,872,793 + ABIE41_RS13985 0.66 -0.2 2,872,793 + ABIE41_RS13985 0.66 -0.8 2,872,793 + ABIE41_RS13985 0.66 +0.3 2,872,794 - ABIE41_RS13985 0.66 -0.7 2,872,794 - ABIE41_RS13985 0.66 +0.8 2,872,794 - ABIE41_RS13985 0.66 -0.8 2,873,030 + ABIE41_RS13985 0.81 -0.8 2,873,030 + ABIE41_RS13985 0.81 -2.0 2,873,030 + ABIE41_RS13985 0.81 +0.3 2,873,030 + ABIE41_RS13985 0.81 -0.2 2,873,030 + ABIE41_RS13985 0.81 -0.7 2,873,030 + ABIE41_RS13985 0.81 -0.1 2,873,030 + ABIE41_RS13985 0.81 -0.0 2,873,031 - ABIE41_RS13985 0.81 +0.0 2,873,117 + ABIE41_RS13985 0.86 -0.1 2,873,117 + ABIE41_RS13985 0.86 +0.5 2,873,118 - ABIE41_RS13985 0.86 -1.5 2,873,118 - ABIE41_RS13985 0.86 -0.5 2,873,118 - ABIE41_RS13985 0.86 -0.9 2,873,118 - ABIE41_RS13985 0.86 +1.5 2,873,118 - ABIE41_RS13985 0.86 +0.3 2,873,267 + -0.4 2,873,267 + +0.0 2,873,267 + -3.8 2,873,268 - +0.3 2,873,321 + +1.2 2,873,321 + -1.2 2,873,321 + -2.1 2,873,321 + -0.2 2,873,322 - -0.1 2,873,322 - -0.6 2,873,322 - -0.7 2,873,693 + ABIE41_RS13990 0.15 +2.3 2,873,693 + ABIE41_RS13990 0.15 +1.6 2,873,693 + ABIE41_RS13990 0.15 +2.3 2,873,694 - ABIE41_RS13990 0.16 +2.0 2,873,694 - ABIE41_RS13990 0.16 +2.1 2,873,932 - -0.3 2,874,161 + ABIE41_RS13995 0.28 +0.0 2,874,161 + ABIE41_RS13995 0.28 -0.2 2,874,161 + ABIE41_RS13995 0.28 -0.5 2,874,161 + ABIE41_RS13995 0.28 -0.3 2,874,162 - ABIE41_RS13995 0.28 -0.8 2,874,162 - ABIE41_RS13995 0.28 -0.4 2,874,162 - ABIE41_RS13995 0.28 +0.1 2,874,162 - ABIE41_RS13995 0.28 -0.5 2,874,257 + ABIE41_RS13995 0.41 +1.0 2,874,258 - ABIE41_RS13995 0.41 +0.6 2,874,291 + ABIE41_RS13995 0.46 +0.2 2,874,291 + ABIE41_RS13995 0.46 -0.6
Or see this region's nucleotide sequence