Experiment: R2A with 2-pentyl-furan 21.3684 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS07705 and ABIE41_RS07710 overlap by 4 nucleotides ABIE41_RS07710 and ABIE41_RS07715 are separated by 110 nucleotides ABIE41_RS07715 and ABIE41_RS07720 are separated by 61 nucleotides
ABIE41_RS07705: ABIE41_RS07705 - phosphonate ABC transporter, permease protein PhnE, at 1,574,488 to 1,575,342
_RS07705
ABIE41_RS07710: ABIE41_RS07710 - phosphonate ABC transporter, permease protein PhnE, at 1,575,339 to 1,576,220
_RS07710
ABIE41_RS07715: ABIE41_RS07715 - phosphonate ABC transporter substrate-binding protein, at 1,576,331 to 1,577,248
_RS07715
ABIE41_RS07720: ABIE41_RS07720 - phosphonate ABC transporter ATP-binding protein, at 1,577,310 to 1,578,128
_RS07720
Position (kb)
1576
1577
1578 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1575.451 kb on + strand, within ABIE41_RS07710 at 1575.557 kb on - strand, within ABIE41_RS07710 at 1575.557 kb on - strand, within ABIE41_RS07710 at 1575.557 kb on - strand, within ABIE41_RS07710 at 1575.873 kb on + strand, within ABIE41_RS07710 at 1575.873 kb on + strand, within ABIE41_RS07710 at 1575.873 kb on + strand, within ABIE41_RS07710 at 1575.873 kb on + strand, within ABIE41_RS07710 at 1575.910 kb on + strand, within ABIE41_RS07710 at 1575.910 kb on + strand, within ABIE41_RS07710 at 1575.910 kb on + strand, within ABIE41_RS07710 at 1575.911 kb on - strand, within ABIE41_RS07710 at 1576.027 kb on + strand, within ABIE41_RS07710 at 1576.027 kb on + strand, within ABIE41_RS07710 at 1576.027 kb on + strand, within ABIE41_RS07710 at 1576.027 kb on + strand, within ABIE41_RS07710 at 1576.027 kb on + strand, within ABIE41_RS07710 at 1576.027 kb on + strand, within ABIE41_RS07710 at 1576.028 kb on - strand, within ABIE41_RS07710 at 1576.165 kb on + strand at 1576.165 kb on + strand at 1576.392 kb on + strand at 1576.392 kb on + strand at 1576.392 kb on + strand at 1576.393 kb on - strand at 1576.393 kb on - strand at 1576.393 kb on - strand at 1576.393 kb on - strand at 1576.393 kb on - strand at 1576.436 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.437 kb on + strand, within ABIE41_RS07715 at 1576.438 kb on - strand, within ABIE41_RS07715 at 1576.438 kb on - strand, within ABIE41_RS07715 at 1576.438 kb on - strand, within ABIE41_RS07715 at 1576.438 kb on - strand, within ABIE41_RS07715 at 1576.530 kb on + strand, within ABIE41_RS07715 at 1576.567 kb on - strand, within ABIE41_RS07715 at 1576.567 kb on - strand, within ABIE41_RS07715 at 1576.567 kb on - strand, within ABIE41_RS07715 at 1576.731 kb on + strand, within ABIE41_RS07715 at 1576.731 kb on + strand, within ABIE41_RS07715 at 1576.732 kb on - strand, within ABIE41_RS07715 at 1576.732 kb on - strand, within ABIE41_RS07715 at 1576.733 kb on - strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.869 kb on + strand, within ABIE41_RS07715 at 1576.870 kb on - strand, within ABIE41_RS07715 at 1576.870 kb on - strand, within ABIE41_RS07715 at 1576.870 kb on - strand, within ABIE41_RS07715 at 1576.870 kb on - strand, within ABIE41_RS07715 at 1576.873 kb on - strand, within ABIE41_RS07715 at 1576.881 kb on + strand, within ABIE41_RS07715 at 1576.884 kb on + strand, within ABIE41_RS07715 at 1576.885 kb on - strand, within ABIE41_RS07715 at 1576.885 kb on - strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.974 kb on + strand, within ABIE41_RS07715 at 1576.975 kb on - strand, within ABIE41_RS07715 at 1576.975 kb on - strand, within ABIE41_RS07715 at 1576.975 kb on - strand, within ABIE41_RS07715 at 1576.975 kb on - strand, within ABIE41_RS07715 at 1576.975 kb on - strand, within ABIE41_RS07715 at 1577.026 kb on - strand, within ABIE41_RS07715 at 1577.076 kb on + strand, within ABIE41_RS07715 at 1577.076 kb on + strand, within ABIE41_RS07715 at 1577.077 kb on - strand, within ABIE41_RS07715 at 1577.077 kb on - strand, within ABIE41_RS07715 at 1577.077 kb on - strand, within ABIE41_RS07715 at 1577.077 kb on - strand, within ABIE41_RS07715 at 1577.094 kb on + strand, within ABIE41_RS07715 at 1577.094 kb on + strand, within ABIE41_RS07715 at 1577.094 kb on + strand, within ABIE41_RS07715 at 1577.155 kb on - strand, within ABIE41_RS07715 at 1577.817 kb on + strand, within ABIE41_RS07720 at 1578.216 kb on + strand at 1578.216 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 2-pentyl-furan 21.3684 mM remove 1,575,451 + ABIE41_RS07710 0.13 -1.5 1,575,557 - ABIE41_RS07710 0.25 -0.1 1,575,557 - ABIE41_RS07710 0.25 +0.1 1,575,557 - ABIE41_RS07710 0.25 -0.1 1,575,873 + ABIE41_RS07710 0.61 -0.4 1,575,873 + ABIE41_RS07710 0.61 +3.9 1,575,873 + ABIE41_RS07710 0.61 +0.2 1,575,873 + ABIE41_RS07710 0.61 +0.6 1,575,910 + ABIE41_RS07710 0.65 +0.2 1,575,910 + ABIE41_RS07710 0.65 +0.1 1,575,910 + ABIE41_RS07710 0.65 +0.5 1,575,911 - ABIE41_RS07710 0.65 +0.1 1,576,027 + ABIE41_RS07710 0.78 +0.1 1,576,027 + ABIE41_RS07710 0.78 +0.8 1,576,027 + ABIE41_RS07710 0.78 +0.8 1,576,027 + ABIE41_RS07710 0.78 -0.9 1,576,027 + ABIE41_RS07710 0.78 -0.6 1,576,027 + ABIE41_RS07710 0.78 +1.8 1,576,028 - ABIE41_RS07710 0.78 +0.0 1,576,165 + -3.3 1,576,165 + -0.6 1,576,392 + +0.5 1,576,392 + +0.7 1,576,392 + +0.3 1,576,393 - +2.9 1,576,393 - +0.6 1,576,393 - +0.8 1,576,393 - -0.6 1,576,393 - +0.7 1,576,436 + ABIE41_RS07715 0.11 -0.4 1,576,437 + ABIE41_RS07715 0.12 +0.1 1,576,437 + ABIE41_RS07715 0.12 +1.7 1,576,437 + ABIE41_RS07715 0.12 -0.0 1,576,437 + ABIE41_RS07715 0.12 -0.2 1,576,437 + ABIE41_RS07715 0.12 +1.0 1,576,437 + ABIE41_RS07715 0.12 -0.4 1,576,437 + ABIE41_RS07715 0.12 -0.1 1,576,438 - ABIE41_RS07715 0.12 -0.2 1,576,438 - ABIE41_RS07715 0.12 +0.5 1,576,438 - ABIE41_RS07715 0.12 +0.2 1,576,438 - ABIE41_RS07715 0.12 +0.3 1,576,530 + ABIE41_RS07715 0.22 -0.5 1,576,567 - ABIE41_RS07715 0.26 +0.2 1,576,567 - ABIE41_RS07715 0.26 +0.5 1,576,567 - ABIE41_RS07715 0.26 +0.3 1,576,731 + ABIE41_RS07715 0.44 -0.4 1,576,731 + ABIE41_RS07715 0.44 +1.6 1,576,732 - ABIE41_RS07715 0.44 +0.4 1,576,732 - ABIE41_RS07715 0.44 +0.5 1,576,733 - ABIE41_RS07715 0.44 +0.3 1,576,869 + ABIE41_RS07715 0.59 -0.6 1,576,869 + ABIE41_RS07715 0.59 +0.6 1,576,869 + ABIE41_RS07715 0.59 +0.4 1,576,869 + ABIE41_RS07715 0.59 +0.4 1,576,869 + ABIE41_RS07715 0.59 +0.9 1,576,869 + ABIE41_RS07715 0.59 +0.6 1,576,869 + ABIE41_RS07715 0.59 +0.6 1,576,869 + ABIE41_RS07715 0.59 -0.5 1,576,869 + ABIE41_RS07715 0.59 -1.4 1,576,870 - ABIE41_RS07715 0.59 +0.7 1,576,870 - ABIE41_RS07715 0.59 +0.8 1,576,870 - ABIE41_RS07715 0.59 +1.1 1,576,870 - ABIE41_RS07715 0.59 -0.4 1,576,873 - ABIE41_RS07715 0.59 +0.3 1,576,881 + ABIE41_RS07715 0.60 +0.4 1,576,884 + ABIE41_RS07715 0.60 -0.1 1,576,885 - ABIE41_RS07715 0.60 -0.7 1,576,885 - ABIE41_RS07715 0.60 -0.9 1,576,974 + ABIE41_RS07715 0.70 -1.2 1,576,974 + ABIE41_RS07715 0.70 -1.4 1,576,974 + ABIE41_RS07715 0.70 -2.4 1,576,974 + ABIE41_RS07715 0.70 -0.5 1,576,974 + ABIE41_RS07715 0.70 -0.1 1,576,974 + ABIE41_RS07715 0.70 +1.0 1,576,974 + ABIE41_RS07715 0.70 -0.1 1,576,974 + ABIE41_RS07715 0.70 +1.1 1,576,974 + ABIE41_RS07715 0.70 +0.2 1,576,974 + ABIE41_RS07715 0.70 -1.1 1,576,974 + ABIE41_RS07715 0.70 +0.9 1,576,974 + ABIE41_RS07715 0.70 +0.5 1,576,974 + ABIE41_RS07715 0.70 -0.6 1,576,975 - ABIE41_RS07715 0.70 -0.2 1,576,975 - ABIE41_RS07715 0.70 -0.6 1,576,975 - ABIE41_RS07715 0.70 -2.2 1,576,975 - ABIE41_RS07715 0.70 +0.8 1,576,975 - ABIE41_RS07715 0.70 -1.0 1,577,026 - ABIE41_RS07715 0.76 +0.6 1,577,076 + ABIE41_RS07715 0.81 -2.1 1,577,076 + ABIE41_RS07715 0.81 -0.7 1,577,077 - ABIE41_RS07715 0.81 +0.5 1,577,077 - ABIE41_RS07715 0.81 -2.4 1,577,077 - ABIE41_RS07715 0.81 +0.6 1,577,077 - ABIE41_RS07715 0.81 +0.5 1,577,094 + ABIE41_RS07715 0.83 -0.8 1,577,094 + ABIE41_RS07715 0.83 +0.8 1,577,094 + ABIE41_RS07715 0.83 +1.0 1,577,155 - ABIE41_RS07715 0.90 +0.0 1,577,817 + ABIE41_RS07720 0.62 -0.3 1,578,216 + -0.7 1,578,216 + +0.4
Or see this region's nucleotide sequence