Experiment: no phage control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS15315 and ABID97_RS15320 are separated by 54 nucleotides ABID97_RS15320 and ABID97_RS15325 overlap by 4 nucleotides ABID97_RS15325 and ABID97_RS15330 are separated by 59 nucleotides ABID97_RS15330 and ABID97_RS15335 overlap by 47 nucleotides
ABID97_RS15315: ABID97_RS15315 - thiazole synthase, at 3,169,090 to 3,169,893
_RS15315
ABID97_RS15320: ABID97_RS15320 - sulfur carrier protein ThiS, at 3,169,948 to 3,170,151
_RS15320
ABID97_RS15325: ABID97_RS15325 - FAD-dependent oxidoreductase, at 3,170,148 to 3,171,239
_RS15325
ABID97_RS15330: ABID97_RS15330 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 3,171,299 to 3,172,159
_RS15330
ABID97_RS15335: ABID97_RS15335 - cytosine permease, at 3,172,113 to 3,173,453
_RS15335
Position (kb) 3170
3171
3172 Strain fitness (log2 ratio) -2
-1
0
1 at 3169.481 kb on + strand, within ABID97_RS15315 at 3169.481 kb on + strand, within ABID97_RS15315 at 3169.559 kb on + strand, within ABID97_RS15315 at 3169.820 kb on + strand at 3169.933 kb on + strand at 3170.141 kb on + strand at 3170.500 kb on + strand, within ABID97_RS15325 at 3170.501 kb on - strand, within ABID97_RS15325
Per-strain Table
Position Strand Gene LocusTag Fraction no phage control remove 3,169,481 + ABID97_RS15315 0.49 +0.3 3,169,481 + ABID97_RS15315 0.49 -0.3 3,169,559 + ABID97_RS15315 0.58 -0.8 3,169,820 + +0.4 3,169,933 + -0.4 3,170,141 + +0.2 3,170,500 + ABID97_RS15325 0.32 -0.5 3,170,501 - ABID97_RS15325 0.32 -1.9
Or see this region's nucleotide sequence