Experiment: R2A with 1-octen-3-ol 0.493390333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS19835 and ABIE41_RS19840 are separated by 55 nucleotides ABIE41_RS19840 and ABIE41_RS19845 are separated by 76 nucleotides ABIE41_RS19845 and ABIE41_RS19850 are separated by 157 nucleotides
ABIE41_RS19835: ABIE41_RS19835 - threonine ammonia-lyase, at 4,070,679 to 4,071,938
_RS19835
ABIE41_RS19840: ABIE41_RS19840 - RDD family protein, at 4,071,994 to 4,072,467
_RS19840
ABIE41_RS19845: ABIE41_RS19845 - arginyltransferase, at 4,072,544 to 4,073,290
_RS19845
ABIE41_RS19850: ABIE41_RS19850 - DUF2336 domain-containing protein, at 4,073,448 to 4,074,473
_RS19850
Position (kb)
4072
4073
4074 Strain fitness (log2 ratio)
-1
0
1
2 at 4072.009 kb on + strand at 4072.351 kb on + strand, within ABIE41_RS19840 at 4072.466 kb on - strand at 4072.505 kb on + strand at 4072.604 kb on + strand at 4072.604 kb on + strand at 4072.868 kb on + strand, within ABIE41_RS19845 at 4072.868 kb on + strand, within ABIE41_RS19845 at 4072.868 kb on + strand, within ABIE41_RS19845 at 4072.868 kb on + strand, within ABIE41_RS19845 at 4072.868 kb on + strand, within ABIE41_RS19845 at 4072.869 kb on - strand, within ABIE41_RS19845 at 4072.869 kb on - strand, within ABIE41_RS19845 at 4072.886 kb on + strand, within ABIE41_RS19845 at 4072.887 kb on - strand, within ABIE41_RS19845 at 4072.989 kb on - strand, within ABIE41_RS19845 at 4073.090 kb on + strand, within ABIE41_RS19845 at 4073.090 kb on + strand, within ABIE41_RS19845 at 4073.090 kb on + strand, within ABIE41_RS19845 at 4073.091 kb on - strand, within ABIE41_RS19845 at 4073.091 kb on - strand, within ABIE41_RS19845 at 4073.091 kb on - strand, within ABIE41_RS19845 at 4073.091 kb on - strand, within ABIE41_RS19845 at 4073.183 kb on + strand, within ABIE41_RS19845 at 4073.183 kb on + strand, within ABIE41_RS19845 at 4073.184 kb on - strand, within ABIE41_RS19845 at 4073.184 kb on - strand, within ABIE41_RS19845 at 4073.184 kb on - strand, within ABIE41_RS19845 at 4073.190 kb on - strand, within ABIE41_RS19845 at 4073.190 kb on - strand, within ABIE41_RS19845 at 4073.228 kb on + strand at 4073.228 kb on + strand at 4073.229 kb on - strand at 4073.350 kb on + strand at 4073.628 kb on + strand, within ABIE41_RS19850 at 4073.629 kb on - strand, within ABIE41_RS19850
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.493390333333333 mM remove 4,072,009 + +1.4 4,072,351 + ABIE41_RS19840 0.75 +1.3 4,072,466 - -0.1 4,072,505 + -0.0 4,072,604 + -0.0 4,072,604 + +0.0 4,072,868 + ABIE41_RS19845 0.43 +0.2 4,072,868 + ABIE41_RS19845 0.43 -0.4 4,072,868 + ABIE41_RS19845 0.43 -1.0 4,072,868 + ABIE41_RS19845 0.43 +0.8 4,072,868 + ABIE41_RS19845 0.43 -0.4 4,072,869 - ABIE41_RS19845 0.44 +0.4 4,072,869 - ABIE41_RS19845 0.44 +0.7 4,072,886 + ABIE41_RS19845 0.46 -0.2 4,072,887 - ABIE41_RS19845 0.46 -0.2 4,072,989 - ABIE41_RS19845 0.60 -0.4 4,073,090 + ABIE41_RS19845 0.73 -1.4 4,073,090 + ABIE41_RS19845 0.73 +0.3 4,073,090 + ABIE41_RS19845 0.73 +0.8 4,073,091 - ABIE41_RS19845 0.73 -0.5 4,073,091 - ABIE41_RS19845 0.73 -0.2 4,073,091 - ABIE41_RS19845 0.73 -0.3 4,073,091 - ABIE41_RS19845 0.73 +0.0 4,073,183 + ABIE41_RS19845 0.86 +0.6 4,073,183 + ABIE41_RS19845 0.86 -0.1 4,073,184 - ABIE41_RS19845 0.86 -0.4 4,073,184 - ABIE41_RS19845 0.86 -0.1 4,073,184 - ABIE41_RS19845 0.86 +0.7 4,073,190 - ABIE41_RS19845 0.86 +1.8 4,073,190 - ABIE41_RS19845 0.86 +0.1 4,073,228 + +1.8 4,073,228 + +0.1 4,073,229 - -0.8 4,073,350 + -1.7 4,073,628 + ABIE41_RS19850 0.18 -0.5 4,073,629 - ABIE41_RS19850 0.18 +0.4
Or see this region's nucleotide sequence