Experiment: phage_S63 5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS01215 and ABID97_RS01220 are separated by 9 nucleotides ABID97_RS01220 and ABID97_RS01225 are separated by 122 nucleotides ABID97_RS01225 and ABID97_RS01230 are separated by 9 nucleotides
ABID97_RS01215: ABID97_RS01215 - tripartite tricarboxylate transporter substrate binding protein, at 249,332 to 250,318
_RS01215
ABID97_RS01220: ABID97_RS01220 - M20 aminoacylase family protein, at 250,328 to 251,509
_RS01220
ABID97_RS01225: ABID97_RS01225 - VOC family protein, at 251,632 to 252,063
_RS01225
ABID97_RS01230: ABID97_RS01230 - amino acid ABC transporter substrate-binding protein, at 252,073 to 252,987
_RS01230
Position (kb) 250
251
252 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 249.596 kb on + strand, within ABID97_RS01215 at 249.600 kb on - strand, within ABID97_RS01215 at 249.600 kb on - strand, within ABID97_RS01215 at 249.600 kb on - strand, within ABID97_RS01215 at 249.600 kb on - strand, within ABID97_RS01215 at 249.600 kb on - strand, within ABID97_RS01215 at 249.828 kb on - strand, within ABID97_RS01215 at 250.085 kb on + strand, within ABID97_RS01215 at 250.598 kb on + strand, within ABID97_RS01220 at 251.364 kb on - strand, within ABID97_RS01220 at 251.364 kb on - strand, within ABID97_RS01220 at 251.367 kb on + strand, within ABID97_RS01220 at 251.367 kb on + strand, within ABID97_RS01220 at 251.368 kb on - strand, within ABID97_RS01220 at 251.557 kb on + strand at 251.561 kb on + strand at 251.592 kb on + strand at 251.948 kb on - strand, within ABID97_RS01225 at 251.948 kb on - strand, within ABID97_RS01225 at 251.948 kb on - strand, within ABID97_RS01225 at 251.948 kb on - strand, within ABID97_RS01225 at 251.948 kb on - strand, within ABID97_RS01225 at 252.247 kb on + strand, within ABID97_RS01230 at 252.247 kb on + strand, within ABID97_RS01230 at 252.248 kb on - strand, within ABID97_RS01230 at 252.248 kb on - strand, within ABID97_RS01230 at 252.248 kb on - strand, within ABID97_RS01230 at 252.331 kb on + strand, within ABID97_RS01230 at 252.455 kb on - strand, within ABID97_RS01230 at 252.455 kb on - strand, within ABID97_RS01230 at 252.455 kb on - strand, within ABID97_RS01230
Per-strain Table
Position Strand Gene LocusTag Fraction phage_S63 5 MOI remove 249,596 + ABID97_RS01215 0.27 +3.2 249,600 - ABID97_RS01215 0.27 +0.4 249,600 - ABID97_RS01215 0.27 -0.4 249,600 - ABID97_RS01215 0.27 +0.3 249,600 - ABID97_RS01215 0.27 +1.7 249,600 - ABID97_RS01215 0.27 -0.5 249,828 - ABID97_RS01215 0.50 +0.5 250,085 + ABID97_RS01215 0.76 -0.3 250,598 + ABID97_RS01220 0.23 -1.8 251,364 - ABID97_RS01220 0.88 +0.9 251,364 - ABID97_RS01220 0.88 +1.7 251,367 + ABID97_RS01220 0.88 -0.6 251,367 + ABID97_RS01220 0.88 +0.4 251,368 - ABID97_RS01220 0.88 -1.2 251,557 + +0.1 251,561 + +1.2 251,592 + +0.9 251,948 - ABID97_RS01225 0.73 +1.0 251,948 - ABID97_RS01225 0.73 -0.0 251,948 - ABID97_RS01225 0.73 +1.6 251,948 - ABID97_RS01225 0.73 +1.2 251,948 - ABID97_RS01225 0.73 -0.3 252,247 + ABID97_RS01230 0.19 +0.3 252,247 + ABID97_RS01230 0.19 -0.3 252,248 - ABID97_RS01230 0.19 +0.8 252,248 - ABID97_RS01230 0.19 -0.9 252,248 - ABID97_RS01230 0.19 -0.4 252,331 + ABID97_RS01230 0.28 -1.0 252,455 - ABID97_RS01230 0.42 +0.9 252,455 - ABID97_RS01230 0.42 +0.3 252,455 - ABID97_RS01230 0.42 -0.2
Or see this region's nucleotide sequence