Experiment: phage_S63 5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS28325 and ABID97_RS28330 are separated by 385 nucleotides ABID97_RS28330 and ABID97_RS28335 overlap by 4 nucleotides ABID97_RS28335 and ABID97_RS28340 are separated by 29 nucleotides
ABID97_RS28325: ABID97_RS28325 - xanthine dehydrogenase family protein molybdopterin-binding subunit, at 584,067 to 586,448
_RS28325
ABID97_RS28330: ABID97_RS28330 - non-heme iron oxygenase ferredoxin subunit, at 586,834 to 587,151
_RS28330
ABID97_RS28335: ABID97_RS28335 - FAD-dependent oxidoreductase, at 587,148 to 588,401
_RS28335
ABID97_RS28340: ABID97_RS28340 - Rieske 2Fe-2S domain-containing protein, at 588,431 to 589,741
_RS28340
Position (kb) 587
588
589 Strain fitness (log2 ratio) -1
0
1 at 586.171 kb on + strand, within ABID97_RS28325 at 586.171 kb on + strand at 586.171 kb on + strand, within ABID97_RS28325 at 586.588 kb on - strand at 588.178 kb on - strand, within ABID97_RS28335 at 588.399 kb on + strand at 588.435 kb on - strand at 588.558 kb on - strand at 588.558 kb on - strand at 588.767 kb on - strand, within ABID97_RS28340
Per-strain Table
Position Strand Gene LocusTag Fraction phage_S63 5 MOI remove 586,171 + ABID97_RS28325 0.88 -0.2 586,171 + +1.0 586,171 + ABID97_RS28325 0.88 -0.3 586,588 - +0.7 588,178 - ABID97_RS28335 0.82 +1.0 588,399 + -0.2 588,435 - -0.1 588,558 - -0.4 588,558 - -0.5 588,767 - ABID97_RS28340 0.26 -0.5
Or see this region's nucleotide sequence