Experiment: phage_S63 5 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS26480 and ABID97_RS26485 are separated by 122 nucleotides ABID97_RS26485 and ABID97_RS26490 are separated by 15 nucleotides ABID97_RS26490 and ABID97_RS26495 overlap by 4 nucleotides
ABID97_RS26480: ABID97_RS26480 - methanol/ethanol family PQQ-dependent dehydrogenase, at 213,705 to 215,519
_RS26480
ABID97_RS26485: ABID97_RS26485 - cytochrome c oxidase assembly protein, at 215,642 to 216,592
_RS26485
ABID97_RS26490: ABID97_RS26490 - aspartate/glutamate racemase family protein, at 216,608 to 217,300
_RS26490
ABID97_RS26495: ABID97_RS26495 - Lrp/AsnC family transcriptional regulator, at 217,297 to 217,773
_RS26495
Position (kb) 215
216
217 Strain fitness (log2 ratio) -1
0
1
2 at 214.672 kb on + strand, within ABID97_RS26480 at 214.672 kb on + strand, within ABID97_RS26480 at 214.673 kb on - strand, within ABID97_RS26480 at 214.673 kb on - strand, within ABID97_RS26480 at 214.697 kb on - strand, within ABID97_RS26480 at 215.083 kb on + strand, within ABID97_RS26480 at 215.083 kb on + strand, within ABID97_RS26480 at 215.227 kb on + strand, within ABID97_RS26480 at 215.227 kb on + strand, within ABID97_RS26480 at 215.228 kb on - strand, within ABID97_RS26480 at 215.228 kb on - strand, within ABID97_RS26480 at 215.228 kb on - strand, within ABID97_RS26480 at 215.228 kb on - strand, within ABID97_RS26480 at 215.554 kb on + strand at 215.555 kb on - strand at 215.644 kb on - strand at 215.830 kb on - strand, within ABID97_RS26485 at 215.898 kb on + strand, within ABID97_RS26485 at 215.899 kb on - strand, within ABID97_RS26485 at 217.210 kb on - strand, within ABID97_RS26490 at 217.460 kb on + strand, within ABID97_RS26495
Per-strain Table
Position Strand Gene LocusTag Fraction phage_S63 5 MOI remove 214,672 + ABID97_RS26480 0.53 -1.5 214,672 + ABID97_RS26480 0.53 -0.0 214,673 - ABID97_RS26480 0.53 +2.0 214,673 - ABID97_RS26480 0.53 -0.3 214,697 - ABID97_RS26480 0.55 -0.5 215,083 + ABID97_RS26480 0.76 -1.0 215,083 + ABID97_RS26480 0.76 -0.4 215,227 + ABID97_RS26480 0.84 -0.9 215,227 + ABID97_RS26480 0.84 +2.0 215,228 - ABID97_RS26480 0.84 -0.3 215,228 - ABID97_RS26480 0.84 +0.2 215,228 - ABID97_RS26480 0.84 +0.7 215,228 - ABID97_RS26480 0.84 -0.0 215,554 + -0.0 215,555 - +0.9 215,644 - -0.2 215,830 - ABID97_RS26485 0.20 -0.8 215,898 + ABID97_RS26485 0.27 -0.1 215,899 - ABID97_RS26485 0.27 -1.1 217,210 - ABID97_RS26490 0.87 -1.4 217,460 + ABID97_RS26495 0.34 -0.7
Or see this region's nucleotide sequence