Experiment: phage_S63 10 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS26300 and ABID97_RS26305 are separated by 30 nucleotides ABID97_RS26305 and ABID97_RS26310 are separated by 351 nucleotides ABID97_RS26310 and ABID97_RS26315 are separated by 20 nucleotides ABID97_RS26315 and ABID97_RS26320 overlap by 4 nucleotides
ABID97_RS26300: ABID97_RS26300 - response regulator, at 184,278 to 184,916
_RS26300
ABID97_RS26305: ABID97_RS26305 - response regulator, at 184,947 to 185,336
_RS26305
ABID97_RS26310: ABID97_RS26310 - PAS domain-containing protein, at 185,688 to 187,202
_RS26310
ABID97_RS26315: ABID97_RS26315 - malonic semialdehyde reductase, at 187,223 to 187,813
_RS26315
ABID97_RS26320: ABID97_RS26320 - alpha/beta hydrolase fold domain-containing protein, at 187,810 to 188,646
_RS26320
Position (kb) 185
186
187
188 Strain fitness (log2 ratio) -2
-1
0
1 at 184.915 kb on - strand at 185.502 kb on + strand at 185.503 kb on - strand at 185.561 kb on + strand at 185.561 kb on + strand at 185.562 kb on - strand at 185.562 kb on - strand at 185.700 kb on + strand at 185.842 kb on - strand, within ABID97_RS26310 at 186.009 kb on + strand, within ABID97_RS26310 at 186.142 kb on - strand, within ABID97_RS26310 at 186.451 kb on - strand, within ABID97_RS26310 at 186.639 kb on - strand, within ABID97_RS26310 at 186.677 kb on - strand, within ABID97_RS26310 at 186.843 kb on - strand, within ABID97_RS26310 at 186.861 kb on - strand, within ABID97_RS26310 at 187.422 kb on + strand, within ABID97_RS26315 at 187.423 kb on - strand, within ABID97_RS26315
Per-strain Table
Position Strand Gene LocusTag Fraction phage_S63 10 MOI remove 184,915 - +1.4 185,502 + -0.0 185,503 - -0.4 185,561 + -0.8 185,561 + +0.1 185,562 - -0.1 185,562 - -0.7 185,700 + +0.7 185,842 - ABID97_RS26310 0.10 +0.9 186,009 + ABID97_RS26310 0.21 +1.5 186,142 - ABID97_RS26310 0.30 -0.1 186,451 - ABID97_RS26310 0.50 +1.6 186,639 - ABID97_RS26310 0.63 -0.1 186,677 - ABID97_RS26310 0.65 +0.5 186,843 - ABID97_RS26310 0.76 -2.1 186,861 - ABID97_RS26310 0.77 -0.4 187,422 + ABID97_RS26315 0.34 +0.5 187,423 - ABID97_RS26315 0.34 -0.8
Or see this region's nucleotide sequence