Experiment: R2A with 1-octen-3-ol 0.493390333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS14925 and ABIE41_RS14930 are separated by 118 nucleotides ABIE41_RS14930 and ABIE41_RS14935 are separated by 103 nucleotides ABIE41_RS14935 and ABIE41_RS14940 are separated by 3 nucleotides ABIE41_RS14940 and ABIE41_RS14945 are separated by 247 nucleotides
ABIE41_RS14925: ABIE41_RS14925 - aminotransferase, at 3,047,867 to 3,049,051
_RS14925
ABIE41_RS14930: ABIE41_RS14930 - Sir2 family NAD-dependent protein deacetylase, at 3,049,170 to 3,049,925
_RS14930
ABIE41_RS14935: ABIE41_RS14935 - DUF3429 domain-containing protein, at 3,050,029 to 3,050,484
_RS14935
ABIE41_RS14940: ABIE41_RS14940 - LysE family translocator, at 3,050,488 to 3,051,120
_RS14940
ABIE41_RS14945: ABIE41_RS14945 - cold-shock protein, at 3,051,368 to 3,051,916
_RS14945
Position (kb)
3050
3051 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3049.153 kb on + strand at 3049.401 kb on + strand, within ABIE41_RS14930 at 3049.401 kb on + strand, within ABIE41_RS14930 at 3049.402 kb on - strand, within ABIE41_RS14930 at 3049.557 kb on + strand, within ABIE41_RS14930 at 3049.557 kb on + strand, within ABIE41_RS14930 at 3049.557 kb on + strand, within ABIE41_RS14930 at 3049.557 kb on + strand, within ABIE41_RS14930 at 3049.557 kb on + strand, within ABIE41_RS14930 at 3049.558 kb on - strand, within ABIE41_RS14930 at 3049.558 kb on - strand, within ABIE41_RS14930 at 3049.558 kb on - strand, within ABIE41_RS14930 at 3049.558 kb on - strand, within ABIE41_RS14930 at 3049.558 kb on - strand, within ABIE41_RS14930 at 3049.558 kb on - strand, within ABIE41_RS14930 at 3049.558 kb on - strand, within ABIE41_RS14930 at 3049.558 kb on - strand, within ABIE41_RS14930 at 3049.558 kb on - strand, within ABIE41_RS14930 at 3049.780 kb on - strand, within ABIE41_RS14930 at 3049.987 kb on + strand at 3049.987 kb on + strand at 3049.987 kb on + strand at 3049.987 kb on + strand at 3049.988 kb on - strand at 3049.988 kb on - strand at 3050.179 kb on + strand, within ABIE41_RS14935 at 3050.180 kb on - strand, within ABIE41_RS14935 at 3050.237 kb on - strand, within ABIE41_RS14935 at 3050.386 kb on + strand, within ABIE41_RS14935 at 3050.387 kb on - strand, within ABIE41_RS14935 at 3050.387 kb on - strand, within ABIE41_RS14935 at 3050.413 kb on + strand, within ABIE41_RS14935 at 3050.873 kb on + strand, within ABIE41_RS14940 at 3050.874 kb on - strand, within ABIE41_RS14940 at 3050.966 kb on + strand, within ABIE41_RS14940 at 3050.966 kb on + strand, within ABIE41_RS14940 at 3050.966 kb on + strand, within ABIE41_RS14940 at 3050.967 kb on - strand, within ABIE41_RS14940 at 3050.967 kb on - strand, within ABIE41_RS14940 at 3050.990 kb on + strand, within ABIE41_RS14940 at 3050.990 kb on + strand, within ABIE41_RS14940 at 3050.990 kb on + strand, within ABIE41_RS14940 at 3050.990 kb on + strand, within ABIE41_RS14940 at 3050.991 kb on - strand, within ABIE41_RS14940 at 3050.991 kb on - strand, within ABIE41_RS14940 at 3051.232 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.493390333333333 mM remove 3,049,153 + +0.1 3,049,401 + ABIE41_RS14930 0.31 -0.1 3,049,401 + ABIE41_RS14930 0.31 +0.2 3,049,402 - ABIE41_RS14930 0.31 -0.8 3,049,557 + ABIE41_RS14930 0.51 +0.1 3,049,557 + ABIE41_RS14930 0.51 +0.1 3,049,557 + ABIE41_RS14930 0.51 +0.1 3,049,557 + ABIE41_RS14930 0.51 +1.1 3,049,557 + ABIE41_RS14930 0.51 -0.1 3,049,558 - ABIE41_RS14930 0.51 +0.5 3,049,558 - ABIE41_RS14930 0.51 +0.6 3,049,558 - ABIE41_RS14930 0.51 +0.1 3,049,558 - ABIE41_RS14930 0.51 +0.6 3,049,558 - ABIE41_RS14930 0.51 -0.1 3,049,558 - ABIE41_RS14930 0.51 +0.4 3,049,558 - ABIE41_RS14930 0.51 +0.9 3,049,558 - ABIE41_RS14930 0.51 +0.5 3,049,558 - ABIE41_RS14930 0.51 +0.3 3,049,780 - ABIE41_RS14930 0.81 -0.6 3,049,987 + +0.9 3,049,987 + +0.4 3,049,987 + -0.3 3,049,987 + +2.0 3,049,988 - -0.3 3,049,988 - +2.0 3,050,179 + ABIE41_RS14935 0.33 -0.2 3,050,180 - ABIE41_RS14935 0.33 -1.0 3,050,237 - ABIE41_RS14935 0.46 -0.7 3,050,386 + ABIE41_RS14935 0.78 +0.2 3,050,387 - ABIE41_RS14935 0.79 +0.1 3,050,387 - ABIE41_RS14935 0.79 -1.3 3,050,413 + ABIE41_RS14935 0.84 +0.1 3,050,873 + ABIE41_RS14940 0.61 +1.0 3,050,874 - ABIE41_RS14940 0.61 -0.5 3,050,966 + ABIE41_RS14940 0.76 +0.2 3,050,966 + ABIE41_RS14940 0.76 -0.8 3,050,966 + ABIE41_RS14940 0.76 -0.1 3,050,967 - ABIE41_RS14940 0.76 +0.1 3,050,967 - ABIE41_RS14940 0.76 +0.9 3,050,990 + ABIE41_RS14940 0.79 -2.4 3,050,990 + ABIE41_RS14940 0.79 +1.3 3,050,990 + ABIE41_RS14940 0.79 -0.1 3,050,990 + ABIE41_RS14940 0.79 +0.5 3,050,991 - ABIE41_RS14940 0.79 -1.2 3,050,991 - ABIE41_RS14940 0.79 -0.2 3,051,232 + -0.9
Or see this region's nucleotide sequence