Experiment: R2A with 1-octen-3-ol 0.493390333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS10565 and ABIE41_RS10570 are separated by 142 nucleotides ABIE41_RS10570 and ABIE41_RS10575 are separated by 15 nucleotides
ABIE41_RS10565: ABIE41_RS10565 - cytochrome-c oxidase, cbb3-type subunit I, at 2,178,648 to 2,180,315
_RS10565
ABIE41_RS10570: ABIE41_RS10570 - 2-hydroxyacid dehydrogenase, at 2,180,458 to 2,181,423
_RS10570
ABIE41_RS10575: ABIE41_RS10575 - gamma-glutamyltransferase, at 2,181,439 to 2,183,115
_RS10575
Position (kb)
2180
2181
2182 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2179.473 kb on - strand, within ABIE41_RS10565 at 2179.473 kb on - strand, within ABIE41_RS10565 at 2179.481 kb on - strand, within ABIE41_RS10565 at 2179.525 kb on + strand, within ABIE41_RS10565 at 2179.525 kb on + strand, within ABIE41_RS10565 at 2179.526 kb on - strand, within ABIE41_RS10565 at 2179.526 kb on - strand, within ABIE41_RS10565 at 2179.526 kb on - strand, within ABIE41_RS10565 at 2179.609 kb on + strand, within ABIE41_RS10565 at 2179.609 kb on + strand, within ABIE41_RS10565 at 2179.610 kb on - strand, within ABIE41_RS10565 at 2179.626 kb on + strand, within ABIE41_RS10565 at 2179.627 kb on + strand, within ABIE41_RS10565 at 2179.627 kb on + strand, within ABIE41_RS10565 at 2179.627 kb on + strand, within ABIE41_RS10565 at 2179.627 kb on + strand, within ABIE41_RS10565 at 2179.627 kb on + strand, within ABIE41_RS10565 at 2179.628 kb on - strand, within ABIE41_RS10565 at 2179.628 kb on - strand, within ABIE41_RS10565 at 2179.628 kb on - strand, within ABIE41_RS10565 at 2179.628 kb on - strand, within ABIE41_RS10565 at 2179.628 kb on - strand, within ABIE41_RS10565 at 2179.678 kb on + strand, within ABIE41_RS10565 at 2179.714 kb on + strand, within ABIE41_RS10565 at 2179.762 kb on + strand, within ABIE41_RS10565 at 2179.795 kb on + strand, within ABIE41_RS10565 at 2179.795 kb on + strand, within ABIE41_RS10565 at 2179.796 kb on - strand, within ABIE41_RS10565 at 2179.864 kb on + strand, within ABIE41_RS10565 at 2179.864 kb on + strand, within ABIE41_RS10565 at 2179.864 kb on + strand, within ABIE41_RS10565 at 2179.864 kb on + strand, within ABIE41_RS10565 at 2180.074 kb on + strand, within ABIE41_RS10565 at 2180.074 kb on + strand, within ABIE41_RS10565 at 2180.074 kb on + strand, within ABIE41_RS10565 at 2180.074 kb on + strand, within ABIE41_RS10565 at 2180.075 kb on - strand, within ABIE41_RS10565 at 2180.075 kb on - strand, within ABIE41_RS10565 at 2180.075 kb on - strand, within ABIE41_RS10565 at 2180.075 kb on - strand, within ABIE41_RS10565 at 2180.075 kb on - strand, within ABIE41_RS10565 at 2180.075 kb on - strand, within ABIE41_RS10565 at 2180.075 kb on - strand, within ABIE41_RS10565 at 2180.194 kb on + strand at 2180.195 kb on - strand at 2180.195 kb on - strand at 2180.372 kb on + strand at 2180.392 kb on - strand at 2180.402 kb on + strand at 2180.439 kb on + strand at 2180.439 kb on + strand at 2180.439 kb on + strand at 2180.440 kb on - strand at 2180.440 kb on - strand at 2180.440 kb on - strand at 2180.636 kb on + strand, within ABIE41_RS10570 at 2180.637 kb on - strand, within ABIE41_RS10570 at 2180.637 kb on - strand, within ABIE41_RS10570 at 2180.637 kb on - strand, within ABIE41_RS10570 at 2180.852 kb on + strand, within ABIE41_RS10570 at 2180.853 kb on - strand, within ABIE41_RS10570 at 2180.906 kb on + strand, within ABIE41_RS10570 at 2180.906 kb on + strand, within ABIE41_RS10570 at 2180.907 kb on - strand, within ABIE41_RS10570 at 2180.907 kb on - strand, within ABIE41_RS10570 at 2181.071 kb on + strand, within ABIE41_RS10570 at 2181.071 kb on + strand, within ABIE41_RS10570 at 2181.071 kb on + strand, within ABIE41_RS10570 at 2181.071 kb on + strand, within ABIE41_RS10570 at 2181.183 kb on + strand, within ABIE41_RS10570 at 2181.183 kb on + strand, within ABIE41_RS10570 at 2181.207 kb on + strand, within ABIE41_RS10570 at 2181.208 kb on - strand, within ABIE41_RS10570 at 2181.208 kb on - strand, within ABIE41_RS10570 at 2181.208 kb on - strand, within ABIE41_RS10570 at 2181.208 kb on - strand, within ABIE41_RS10570 at 2181.208 kb on - strand, within ABIE41_RS10570 at 2181.278 kb on + strand, within ABIE41_RS10570 at 2181.368 kb on - strand at 2181.896 kb on + strand, within ABIE41_RS10575 at 2181.896 kb on + strand, within ABIE41_RS10575 at 2181.896 kb on + strand, within ABIE41_RS10575 at 2181.896 kb on + strand, within ABIE41_RS10575 at 2181.896 kb on + strand, within ABIE41_RS10575 at 2181.896 kb on + strand, within ABIE41_RS10575 at 2181.896 kb on + strand, within ABIE41_RS10575 at 2181.896 kb on + strand, within ABIE41_RS10575 at 2181.896 kb on + strand, within ABIE41_RS10575 at 2181.896 kb on + strand, within ABIE41_RS10575 at 2181.896 kb on + strand, within ABIE41_RS10575 at 2181.896 kb on + strand, within ABIE41_RS10575 at 2181.897 kb on - strand, within ABIE41_RS10575 at 2181.897 kb on - strand, within ABIE41_RS10575 at 2181.897 kb on - strand, within ABIE41_RS10575 at 2181.897 kb on - strand, within ABIE41_RS10575 at 2181.897 kb on - strand, within ABIE41_RS10575 at 2181.897 kb on - strand, within ABIE41_RS10575 at 2181.897 kb on - strand, within ABIE41_RS10575 at 2181.897 kb on - strand, within ABIE41_RS10575 at 2182.097 kb on + strand, within ABIE41_RS10575 at 2182.097 kb on + strand, within ABIE41_RS10575 at 2182.097 kb on + strand, within ABIE41_RS10575 at 2182.097 kb on + strand, within ABIE41_RS10575 at 2182.098 kb on - strand, within ABIE41_RS10575 at 2182.098 kb on - strand, within ABIE41_RS10575 at 2182.275 kb on + strand, within ABIE41_RS10575 at 2182.329 kb on - strand, within ABIE41_RS10575 at 2182.329 kb on - strand, within ABIE41_RS10575 at 2182.329 kb on - strand, within ABIE41_RS10575 at 2182.398 kb on + strand, within ABIE41_RS10575 at 2182.398 kb on + strand, within ABIE41_RS10575
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.493390333333333 mM remove 2,179,473 - ABIE41_RS10565 0.49 +0.2 2,179,473 - ABIE41_RS10565 0.49 -0.9 2,179,481 - ABIE41_RS10565 0.50 +0.7 2,179,525 + ABIE41_RS10565 0.53 +1.2 2,179,525 + ABIE41_RS10565 0.53 -0.2 2,179,526 - ABIE41_RS10565 0.53 +0.1 2,179,526 - ABIE41_RS10565 0.53 -0.3 2,179,526 - ABIE41_RS10565 0.53 -0.3 2,179,609 + ABIE41_RS10565 0.58 -1.4 2,179,609 + ABIE41_RS10565 0.58 +0.1 2,179,610 - ABIE41_RS10565 0.58 +0.5 2,179,626 + ABIE41_RS10565 0.59 -0.4 2,179,627 + ABIE41_RS10565 0.59 -0.1 2,179,627 + ABIE41_RS10565 0.59 -0.2 2,179,627 + ABIE41_RS10565 0.59 -3.0 2,179,627 + ABIE41_RS10565 0.59 +1.0 2,179,627 + ABIE41_RS10565 0.59 +0.8 2,179,628 - ABIE41_RS10565 0.59 -0.8 2,179,628 - ABIE41_RS10565 0.59 +0.6 2,179,628 - ABIE41_RS10565 0.59 -1.3 2,179,628 - ABIE41_RS10565 0.59 -0.2 2,179,628 - ABIE41_RS10565 0.59 +0.1 2,179,678 + ABIE41_RS10565 0.62 +0.2 2,179,714 + ABIE41_RS10565 0.64 +0.3 2,179,762 + ABIE41_RS10565 0.67 +0.4 2,179,795 + ABIE41_RS10565 0.69 +1.0 2,179,795 + ABIE41_RS10565 0.69 -0.3 2,179,796 - ABIE41_RS10565 0.69 +0.5 2,179,864 + ABIE41_RS10565 0.73 -0.2 2,179,864 + ABIE41_RS10565 0.73 -0.2 2,179,864 + ABIE41_RS10565 0.73 -1.5 2,179,864 + ABIE41_RS10565 0.73 +0.2 2,180,074 + ABIE41_RS10565 0.85 -1.3 2,180,074 + ABIE41_RS10565 0.85 +2.4 2,180,074 + ABIE41_RS10565 0.85 -0.2 2,180,074 + ABIE41_RS10565 0.85 +0.6 2,180,075 - ABIE41_RS10565 0.86 -1.0 2,180,075 - ABIE41_RS10565 0.86 -0.7 2,180,075 - ABIE41_RS10565 0.86 -1.2 2,180,075 - ABIE41_RS10565 0.86 -1.5 2,180,075 - ABIE41_RS10565 0.86 +0.2 2,180,075 - ABIE41_RS10565 0.86 +0.8 2,180,075 - ABIE41_RS10565 0.86 +0.6 2,180,194 + +0.4 2,180,195 - -0.2 2,180,195 - -0.5 2,180,372 + -1.2 2,180,392 - +0.1 2,180,402 + -2.2 2,180,439 + +0.1 2,180,439 + -1.5 2,180,439 + -0.2 2,180,440 - +0.0 2,180,440 - -0.1 2,180,440 - +0.7 2,180,636 + ABIE41_RS10570 0.18 +0.9 2,180,637 - ABIE41_RS10570 0.19 +0.9 2,180,637 - ABIE41_RS10570 0.19 +0.1 2,180,637 - ABIE41_RS10570 0.19 -0.2 2,180,852 + ABIE41_RS10570 0.41 -0.0 2,180,853 - ABIE41_RS10570 0.41 +1.2 2,180,906 + ABIE41_RS10570 0.46 -0.8 2,180,906 + ABIE41_RS10570 0.46 -1.0 2,180,907 - ABIE41_RS10570 0.46 -0.4 2,180,907 - ABIE41_RS10570 0.46 -0.3 2,181,071 + ABIE41_RS10570 0.63 -0.5 2,181,071 + ABIE41_RS10570 0.63 +0.6 2,181,071 + ABIE41_RS10570 0.63 +0.8 2,181,071 + ABIE41_RS10570 0.63 -1.0 2,181,183 + ABIE41_RS10570 0.75 -1.0 2,181,183 + ABIE41_RS10570 0.75 -0.2 2,181,207 + ABIE41_RS10570 0.78 -0.7 2,181,208 - ABIE41_RS10570 0.78 +0.5 2,181,208 - ABIE41_RS10570 0.78 -0.2 2,181,208 - ABIE41_RS10570 0.78 +0.4 2,181,208 - ABIE41_RS10570 0.78 +0.0 2,181,208 - ABIE41_RS10570 0.78 -0.7 2,181,278 + ABIE41_RS10570 0.85 +0.2 2,181,368 - +0.0 2,181,896 + ABIE41_RS10575 0.27 -0.1 2,181,896 + ABIE41_RS10575 0.27 +0.4 2,181,896 + ABIE41_RS10575 0.27 +1.5 2,181,896 + ABIE41_RS10575 0.27 +1.3 2,181,896 + ABIE41_RS10575 0.27 -1.0 2,181,896 + ABIE41_RS10575 0.27 +0.1 2,181,896 + ABIE41_RS10575 0.27 +0.6 2,181,896 + ABIE41_RS10575 0.27 +2.2 2,181,896 + ABIE41_RS10575 0.27 +0.8 2,181,896 + ABIE41_RS10575 0.27 -0.4 2,181,896 + ABIE41_RS10575 0.27 +0.6 2,181,896 + ABIE41_RS10575 0.27 -0.0 2,181,897 - ABIE41_RS10575 0.27 -0.7 2,181,897 - ABIE41_RS10575 0.27 -0.3 2,181,897 - ABIE41_RS10575 0.27 -1.0 2,181,897 - ABIE41_RS10575 0.27 +0.1 2,181,897 - ABIE41_RS10575 0.27 -0.1 2,181,897 - ABIE41_RS10575 0.27 -0.0 2,181,897 - ABIE41_RS10575 0.27 -0.5 2,181,897 - ABIE41_RS10575 0.27 -0.1 2,182,097 + ABIE41_RS10575 0.39 -1.7 2,182,097 + ABIE41_RS10575 0.39 -0.9 2,182,097 + ABIE41_RS10575 0.39 +0.1 2,182,097 + ABIE41_RS10575 0.39 +0.0 2,182,098 - ABIE41_RS10575 0.39 +0.3 2,182,098 - ABIE41_RS10575 0.39 -0.3 2,182,275 + ABIE41_RS10575 0.50 +0.3 2,182,329 - ABIE41_RS10575 0.53 -1.9 2,182,329 - ABIE41_RS10575 0.53 -0.5 2,182,329 - ABIE41_RS10575 0.53 -1.3 2,182,398 + ABIE41_RS10575 0.57 -0.0 2,182,398 + ABIE41_RS10575 0.57 +0.3
Or see this region's nucleotide sequence