Experiment: R2A with 1-octen-3-ol 0.493390333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS01765 and ABIE41_RS01770 are separated by 7 nucleotides ABIE41_RS01770 and ABIE41_RS01775 are separated by 426 nucleotides ABIE41_RS01775 and ABIE41_RS01780 are separated by 13 nucleotides ABIE41_RS01780 and ABIE41_RS01785 overlap by 4 nucleotides
ABIE41_RS01765: ABIE41_RS01765 - cob(I)yrinic acid a,c-diamide adenosyltransferase, at 357,832 to 358,443
_RS01765
ABIE41_RS01770: ABIE41_RS01770 - bifunctional adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase, at 358,451 to 358,969
_RS01770
ABIE41_RS01775: ABIE41_RS01775 - HupE/UreJ family protein, at 359,396 to 360,019
_RS01775
ABIE41_RS01780: ABIE41_RS01780 - DUF1636 domain-containing protein, at 360,033 to 360,452
_RS01780
ABIE41_RS01785: ABIE41_RS01785 - cobalamin biosynthesis protein CobW, at 360,449 to 361,486
_RS01785
Position (kb)
359
360
361 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 358.413 kb on - strand at 358.413 kb on - strand at 358.413 kb on - strand at 358.458 kb on - strand at 359.090 kb on + strand at 359.169 kb on + strand at 359.169 kb on + strand at 359.169 kb on + strand at 359.169 kb on + strand at 359.169 kb on + strand at 359.272 kb on + strand at 359.272 kb on + strand at 359.351 kb on + strand at 359.351 kb on + strand at 359.351 kb on + strand at 359.351 kb on + strand at 359.351 kb on + strand at 359.351 kb on + strand at 359.585 kb on + strand, within ABIE41_RS01775 at 359.653 kb on + strand, within ABIE41_RS01775 at 359.653 kb on + strand, within ABIE41_RS01775 at 360.046 kb on + strand at 360.046 kb on + strand at 360.047 kb on - strand at 360.047 kb on - strand at 360.282 kb on + strand, within ABIE41_RS01780 at 360.282 kb on + strand, within ABIE41_RS01780 at 360.282 kb on + strand, within ABIE41_RS01780 at 360.282 kb on + strand, within ABIE41_RS01780 at 360.282 kb on + strand, within ABIE41_RS01780 at 360.282 kb on + strand, within ABIE41_RS01780 at 360.282 kb on + strand, within ABIE41_RS01780 at 360.282 kb on + strand, within ABIE41_RS01780 at 360.282 kb on + strand, within ABIE41_RS01780 at 360.282 kb on + strand, within ABIE41_RS01780 at 360.282 kb on + strand, within ABIE41_RS01780 at 360.288 kb on + strand, within ABIE41_RS01780 at 360.288 kb on + strand, within ABIE41_RS01780 at 360.288 kb on + strand, within ABIE41_RS01780 at 360.288 kb on + strand, within ABIE41_RS01780 at 360.288 kb on + strand, within ABIE41_RS01780
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.493390333333333 mM remove 358,413 - -1.4 358,413 - -0.5 358,413 - -3.4 358,458 - +0.1 359,090 + -0.2 359,169 + -1.3 359,169 + -0.2 359,169 + +0.7 359,169 + +2.2 359,169 + +0.4 359,272 + +0.2 359,272 + +0.6 359,351 + -0.4 359,351 + -0.5 359,351 + +1.7 359,351 + +2.0 359,351 + -0.1 359,351 + -0.0 359,585 + ABIE41_RS01775 0.30 -0.9 359,653 + ABIE41_RS01775 0.41 -0.5 359,653 + ABIE41_RS01775 0.41 -0.3 360,046 + -1.3 360,046 + -0.0 360,047 - -1.6 360,047 - -0.3 360,282 + ABIE41_RS01780 0.59 -3.0 360,282 + ABIE41_RS01780 0.59 +1.8 360,282 + ABIE41_RS01780 0.59 +0.1 360,282 + ABIE41_RS01780 0.59 +0.6 360,282 + ABIE41_RS01780 0.59 +0.1 360,282 + ABIE41_RS01780 0.59 -0.1 360,282 + ABIE41_RS01780 0.59 +0.1 360,282 + ABIE41_RS01780 0.59 -0.9 360,282 + ABIE41_RS01780 0.59 +0.7 360,282 + ABIE41_RS01780 0.59 +0.0 360,282 + ABIE41_RS01780 0.59 +1.3 360,288 + ABIE41_RS01780 0.61 -2.7 360,288 + ABIE41_RS01780 0.61 -1.2 360,288 + ABIE41_RS01780 0.61 +0.5 360,288 + ABIE41_RS01780 0.61 -0.7 360,288 + ABIE41_RS01780 0.61 +1.1
Or see this region's nucleotide sequence