Experiment: R2A with 1-octen-3-ol 0.542729333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS10525 and ABIE41_RS10530 are separated by 96 nucleotides ABIE41_RS10530 and ABIE41_RS10535 overlap by 4 nucleotides ABIE41_RS10535 and ABIE41_RS10540 are separated by 4 nucleotides ABIE41_RS10540 and ABIE41_RS10545 are separated by 35 nucleotides
ABIE41_RS10525: ABIE41_RS10525 - NAD-dependent epimerase, at 2,171,263 to 2,172,291
_RS10525
ABIE41_RS10530: ABIE41_RS10530 - cbb3-type cytochrome oxidase assembly protein CcoS, at 2,172,388 to 2,172,603
_RS10530
ABIE41_RS10535: ABIE41_RS10535 - heavy metal translocating P-type ATPase, at 2,172,600 to 2,174,807
_RS10535
ABIE41_RS10540: ABIE41_RS10540 - FixH family protein, at 2,174,812 to 2,175,336
_RS10540
ABIE41_RS10545: ABIE41_RS10545 - cytochrome c oxidase accessory protein CcoG, at 2,175,372 to 2,176,814
_RS10545
Position (kb)
2172
2173
2174
2175 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2171.615 kb on + strand, within ABIE41_RS10525 at 2171.616 kb on - strand, within ABIE41_RS10525 at 2171.616 kb on - strand, within ABIE41_RS10525 at 2171.648 kb on + strand, within ABIE41_RS10525 at 2171.765 kb on + strand, within ABIE41_RS10525 at 2171.777 kb on + strand, within ABIE41_RS10525 at 2171.903 kb on + strand, within ABIE41_RS10525 at 2171.903 kb on + strand, within ABIE41_RS10525 at 2171.904 kb on - strand, within ABIE41_RS10525 at 2172.080 kb on + strand, within ABIE41_RS10525 at 2172.080 kb on + strand, within ABIE41_RS10525 at 2172.163 kb on + strand, within ABIE41_RS10525 at 2172.164 kb on + strand, within ABIE41_RS10525 at 2172.513 kb on - strand, within ABIE41_RS10530 at 2172.752 kb on - strand at 2173.638 kb on + strand, within ABIE41_RS10535 at 2173.638 kb on + strand, within ABIE41_RS10535 at 2173.638 kb on + strand, within ABIE41_RS10535 at 2173.638 kb on + strand, within ABIE41_RS10535 at 2173.638 kb on + strand, within ABIE41_RS10535 at 2173.638 kb on + strand, within ABIE41_RS10535 at 2173.638 kb on + strand, within ABIE41_RS10535 at 2173.639 kb on - strand, within ABIE41_RS10535 at 2173.639 kb on - strand, within ABIE41_RS10535 at 2173.639 kb on - strand, within ABIE41_RS10535 at 2173.639 kb on - strand, within ABIE41_RS10535 at 2173.738 kb on + strand, within ABIE41_RS10535 at 2173.739 kb on - strand, within ABIE41_RS10535 at 2174.055 kb on + strand, within ABIE41_RS10535 at 2174.055 kb on + strand, within ABIE41_RS10535 at 2174.055 kb on + strand, within ABIE41_RS10535 at 2174.055 kb on + strand, within ABIE41_RS10535 at 2174.158 kb on + strand, within ABIE41_RS10535 at 2174.206 kb on + strand, within ABIE41_RS10535 at 2174.207 kb on - strand, within ABIE41_RS10535 at 2174.207 kb on - strand, within ABIE41_RS10535 at 2174.347 kb on + strand, within ABIE41_RS10535 at 2174.347 kb on + strand, within ABIE41_RS10535 at 2174.348 kb on - strand, within ABIE41_RS10535 at 2174.491 kb on + strand, within ABIE41_RS10535 at 2174.492 kb on - strand, within ABIE41_RS10535 at 2174.492 kb on - strand, within ABIE41_RS10535 at 2174.554 kb on + strand, within ABIE41_RS10535 at 2174.554 kb on + strand, within ABIE41_RS10535 at 2174.775 kb on + strand at 2174.776 kb on - strand at 2174.924 kb on + strand, within ABIE41_RS10540 at 2174.925 kb on - strand, within ABIE41_RS10540 at 2175.143 kb on + strand, within ABIE41_RS10540 at 2175.143 kb on + strand, within ABIE41_RS10540 at 2175.144 kb on - strand, within ABIE41_RS10540 at 2175.144 kb on - strand, within ABIE41_RS10540 at 2175.162 kb on - strand, within ABIE41_RS10540 at 2175.162 kb on - strand, within ABIE41_RS10540 at 2175.162 kb on - strand, within ABIE41_RS10540 at 2175.162 kb on - strand, within ABIE41_RS10540 at 2175.162 kb on - strand, within ABIE41_RS10540 at 2175.162 kb on - strand, within ABIE41_RS10540 at 2175.649 kb on + strand, within ABIE41_RS10545 at 2175.784 kb on + strand, within ABIE41_RS10545 at 2175.784 kb on + strand, within ABIE41_RS10545 at 2175.784 kb on + strand, within ABIE41_RS10545 at 2175.784 kb on + strand, within ABIE41_RS10545 at 2175.784 kb on + strand, within ABIE41_RS10545 at 2175.784 kb on + strand, within ABIE41_RS10545 at 2175.785 kb on - strand, within ABIE41_RS10545 at 2175.785 kb on - strand, within ABIE41_RS10545 at 2175.785 kb on - strand, within ABIE41_RS10545 at 2175.785 kb on - strand, within ABIE41_RS10545
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.542729333333333 mM remove 2,171,615 + ABIE41_RS10525 0.34 -2.5 2,171,616 - ABIE41_RS10525 0.34 -1.1 2,171,616 - ABIE41_RS10525 0.34 -0.7 2,171,648 + ABIE41_RS10525 0.37 -2.7 2,171,765 + ABIE41_RS10525 0.49 -3.4 2,171,777 + ABIE41_RS10525 0.50 -0.5 2,171,903 + ABIE41_RS10525 0.62 -1.8 2,171,903 + ABIE41_RS10525 0.62 -2.0 2,171,904 - ABIE41_RS10525 0.62 -1.7 2,172,080 + ABIE41_RS10525 0.79 -1.5 2,172,080 + ABIE41_RS10525 0.79 -1.8 2,172,163 + ABIE41_RS10525 0.87 -3.2 2,172,164 + ABIE41_RS10525 0.88 +1.9 2,172,513 - ABIE41_RS10530 0.58 +0.1 2,172,752 - +0.4 2,173,638 + ABIE41_RS10535 0.47 +0.0 2,173,638 + ABIE41_RS10535 0.47 +0.2 2,173,638 + ABIE41_RS10535 0.47 -0.5 2,173,638 + ABIE41_RS10535 0.47 -1.3 2,173,638 + ABIE41_RS10535 0.47 -0.4 2,173,638 + ABIE41_RS10535 0.47 -0.1 2,173,638 + ABIE41_RS10535 0.47 -0.6 2,173,639 - ABIE41_RS10535 0.47 -0.1 2,173,639 - ABIE41_RS10535 0.47 +0.4 2,173,639 - ABIE41_RS10535 0.47 +0.8 2,173,639 - ABIE41_RS10535 0.47 +0.9 2,173,738 + ABIE41_RS10535 0.52 +0.0 2,173,739 - ABIE41_RS10535 0.52 -2.5 2,174,055 + ABIE41_RS10535 0.66 -0.7 2,174,055 + ABIE41_RS10535 0.66 -1.8 2,174,055 + ABIE41_RS10535 0.66 -0.3 2,174,055 + ABIE41_RS10535 0.66 -1.8 2,174,158 + ABIE41_RS10535 0.71 +1.3 2,174,206 + ABIE41_RS10535 0.73 +0.0 2,174,207 - ABIE41_RS10535 0.73 +0.2 2,174,207 - ABIE41_RS10535 0.73 -0.4 2,174,347 + ABIE41_RS10535 0.79 -0.3 2,174,347 + ABIE41_RS10535 0.79 -0.8 2,174,348 - ABIE41_RS10535 0.79 +0.9 2,174,491 + ABIE41_RS10535 0.86 -0.2 2,174,492 - ABIE41_RS10535 0.86 -0.9 2,174,492 - ABIE41_RS10535 0.86 -0.2 2,174,554 + ABIE41_RS10535 0.88 -0.4 2,174,554 + ABIE41_RS10535 0.88 -0.3 2,174,775 + -0.4 2,174,776 - -0.2 2,174,924 + ABIE41_RS10540 0.21 -0.9 2,174,925 - ABIE41_RS10540 0.22 -0.1 2,175,143 + ABIE41_RS10540 0.63 +0.0 2,175,143 + ABIE41_RS10540 0.63 +0.1 2,175,144 - ABIE41_RS10540 0.63 -1.0 2,175,144 - ABIE41_RS10540 0.63 -1.5 2,175,162 - ABIE41_RS10540 0.67 -0.5 2,175,162 - ABIE41_RS10540 0.67 +0.7 2,175,162 - ABIE41_RS10540 0.67 -2.0 2,175,162 - ABIE41_RS10540 0.67 +0.3 2,175,162 - ABIE41_RS10540 0.67 +0.4 2,175,162 - ABIE41_RS10540 0.67 -0.6 2,175,649 + ABIE41_RS10545 0.19 +0.6 2,175,784 + ABIE41_RS10545 0.29 +0.1 2,175,784 + ABIE41_RS10545 0.29 +0.0 2,175,784 + ABIE41_RS10545 0.29 -2.3 2,175,784 + ABIE41_RS10545 0.29 +1.1 2,175,784 + ABIE41_RS10545 0.29 +0.2 2,175,784 + ABIE41_RS10545 0.29 +1.4 2,175,785 - ABIE41_RS10545 0.29 -0.0 2,175,785 - ABIE41_RS10545 0.29 +0.4 2,175,785 - ABIE41_RS10545 0.29 -0.5 2,175,785 - ABIE41_RS10545 0.29 -1.4
Or see this region's nucleotide sequence