Experiment: R2A with 1-octen-3-ol 0.542729333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS15320 and ABIE41_RS15325 are separated by 111 nucleotides ABIE41_RS15325 and ABIE41_RS15330 are separated by 49 nucleotides ABIE41_RS15330 and ABIE41_RS15335 overlap by 14 nucleotides ABIE41_RS15335 and ABIE41_RS15340 are separated by 3 nucleotides ABIE41_RS15340 and ABIE41_RS15345 are separated by 203 nucleotides
ABIE41_RS15320: ABIE41_RS15320 - FAD-dependent oxidoreductase, at 3,133,820 to 3,134,767
_RS15320
ABIE41_RS15325: ABIE41_RS15325 - hypothetical protein, at 3,134,879 to 3,135,034
_RS15325
ABIE41_RS15330: ABIE41_RS15330 - DUF934 domain-containing protein, at 3,135,084 to 3,135,614
_RS15330
ABIE41_RS15335: ABIE41_RS15335 - nitrite/sulfite reductase, at 3,135,601 to 3,137,271
_RS15335
ABIE41_RS15340: ABIE41_RS15340 - DUF2849 domain-containing protein, at 3,137,275 to 3,137,631
_RS15340
ABIE41_RS15345: ABIE41_RS15345 - sulfate/molybdate ABC transporter ATP-binding protein, at 3,137,835 to 3,138,881
_RS15345
Position (kb)
3135
3136
3137
3138 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3134.607 kb on - strand, within ABIE41_RS15320 at 3135.082 kb on - strand at 3135.082 kb on - strand at 3135.082 kb on - strand at 3135.082 kb on - strand at 3135.082 kb on - strand at 3135.175 kb on + strand, within ABIE41_RS15330 at 3135.176 kb on - strand, within ABIE41_RS15330 at 3135.176 kb on - strand, within ABIE41_RS15330 at 3135.280 kb on + strand, within ABIE41_RS15330 at 3135.280 kb on + strand, within ABIE41_RS15330 at 3135.281 kb on - strand, within ABIE41_RS15330 at 3135.365 kb on - strand, within ABIE41_RS15330 at 3135.448 kb on - strand, within ABIE41_RS15330 at 3135.451 kb on + strand, within ABIE41_RS15330 at 3135.452 kb on + strand, within ABIE41_RS15330 at 3135.707 kb on + strand at 3135.707 kb on + strand at 3135.708 kb on - strand at 3135.708 kb on - strand at 3136.010 kb on + strand, within ABIE41_RS15335 at 3136.010 kb on + strand, within ABIE41_RS15335 at 3136.011 kb on - strand, within ABIE41_RS15335 at 3136.173 kb on - strand, within ABIE41_RS15335 at 3136.268 kb on + strand, within ABIE41_RS15335 at 3136.268 kb on + strand, within ABIE41_RS15335 at 3136.268 kb on + strand, within ABIE41_RS15335 at 3136.269 kb on - strand, within ABIE41_RS15335 at 3136.377 kb on - strand, within ABIE41_RS15335 at 3136.394 kb on + strand, within ABIE41_RS15335 at 3136.395 kb on - strand, within ABIE41_RS15335 at 3136.479 kb on + strand, within ABIE41_RS15335 at 3136.530 kb on - strand, within ABIE41_RS15335 at 3136.530 kb on - strand, within ABIE41_RS15335 at 3136.530 kb on - strand, within ABIE41_RS15335 at 3136.530 kb on - strand, within ABIE41_RS15335 at 3136.530 kb on - strand, within ABIE41_RS15335 at 3136.559 kb on + strand, within ABIE41_RS15335 at 3136.560 kb on - strand, within ABIE41_RS15335 at 3136.560 kb on - strand, within ABIE41_RS15335 at 3136.791 kb on - strand, within ABIE41_RS15335 at 3136.791 kb on - strand, within ABIE41_RS15335 at 3136.791 kb on - strand, within ABIE41_RS15335 at 3136.791 kb on - strand, within ABIE41_RS15335 at 3136.791 kb on - strand, within ABIE41_RS15335 at 3137.040 kb on - strand, within ABIE41_RS15335 at 3137.040 kb on - strand, within ABIE41_RS15335 at 3137.054 kb on + strand, within ABIE41_RS15335 at 3137.055 kb on - strand, within ABIE41_RS15335 at 3137.115 kb on + strand at 3137.115 kb on + strand at 3137.115 kb on + strand at 3137.115 kb on + strand at 3137.116 kb on - strand at 3137.116 kb on - strand at 3137.116 kb on - strand at 3137.116 kb on - strand at 3137.117 kb on + strand at 3137.117 kb on + strand at 3137.118 kb on - strand at 3137.118 kb on - strand at 3137.118 kb on - strand at 3137.118 kb on - strand at 3137.118 kb on - strand at 3137.118 kb on - strand at 3137.118 kb on - strand at 3137.136 kb on - strand at 3137.136 kb on - strand at 3137.267 kb on + strand at 3137.268 kb on - strand at 3137.506 kb on + strand, within ABIE41_RS15340 at 3137.727 kb on + strand at 3138.055 kb on + strand, within ABIE41_RS15345 at 3138.056 kb on - strand, within ABIE41_RS15345 at 3138.056 kb on - strand, within ABIE41_RS15345 at 3138.056 kb on - strand, within ABIE41_RS15345 at 3138.202 kb on + strand, within ABIE41_RS15345 at 3138.203 kb on - strand, within ABIE41_RS15345 at 3138.203 kb on - strand, within ABIE41_RS15345
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.542729333333333 mM remove 3,134,607 - ABIE41_RS15320 0.83 +0.4 3,135,082 - +0.4 3,135,082 - -1.5 3,135,082 - -0.6 3,135,082 - -0.6 3,135,082 - +0.2 3,135,175 + ABIE41_RS15330 0.17 +0.8 3,135,176 - ABIE41_RS15330 0.17 +0.4 3,135,176 - ABIE41_RS15330 0.17 +0.5 3,135,280 + ABIE41_RS15330 0.37 +0.2 3,135,280 + ABIE41_RS15330 0.37 +0.5 3,135,281 - ABIE41_RS15330 0.37 -2.0 3,135,365 - ABIE41_RS15330 0.53 -0.8 3,135,448 - ABIE41_RS15330 0.69 -1.2 3,135,451 + ABIE41_RS15330 0.69 +1.9 3,135,452 + ABIE41_RS15330 0.69 +0.4 3,135,707 + -0.3 3,135,707 + +0.1 3,135,708 - -0.2 3,135,708 - -0.3 3,136,010 + ABIE41_RS15335 0.24 -0.2 3,136,010 + ABIE41_RS15335 0.24 +0.8 3,136,011 - ABIE41_RS15335 0.25 +0.1 3,136,173 - ABIE41_RS15335 0.34 -0.4 3,136,268 + ABIE41_RS15335 0.40 -1.2 3,136,268 + ABIE41_RS15335 0.40 -0.4 3,136,268 + ABIE41_RS15335 0.40 -0.2 3,136,269 - ABIE41_RS15335 0.40 -0.5 3,136,377 - ABIE41_RS15335 0.46 +0.7 3,136,394 + ABIE41_RS15335 0.47 +0.2 3,136,395 - ABIE41_RS15335 0.48 +0.5 3,136,479 + ABIE41_RS15335 0.53 -0.6 3,136,530 - ABIE41_RS15335 0.56 -1.4 3,136,530 - ABIE41_RS15335 0.56 -0.3 3,136,530 - ABIE41_RS15335 0.56 -1.8 3,136,530 - ABIE41_RS15335 0.56 -0.2 3,136,530 - ABIE41_RS15335 0.56 -1.9 3,136,559 + ABIE41_RS15335 0.57 -0.2 3,136,560 - ABIE41_RS15335 0.57 +1.1 3,136,560 - ABIE41_RS15335 0.57 -2.3 3,136,791 - ABIE41_RS15335 0.71 -1.1 3,136,791 - ABIE41_RS15335 0.71 -0.1 3,136,791 - ABIE41_RS15335 0.71 +0.0 3,136,791 - ABIE41_RS15335 0.71 -1.9 3,136,791 - ABIE41_RS15335 0.71 -0.5 3,137,040 - ABIE41_RS15335 0.86 +0.4 3,137,040 - ABIE41_RS15335 0.86 -1.2 3,137,054 + ABIE41_RS15335 0.87 -2.1 3,137,055 - ABIE41_RS15335 0.87 +0.1 3,137,115 + -3.0 3,137,115 + +0.4 3,137,115 + -0.1 3,137,115 + -0.2 3,137,116 - -0.1 3,137,116 - +0.6 3,137,116 - +0.6 3,137,116 - -0.5 3,137,117 + +0.5 3,137,117 + +0.8 3,137,118 - +0.1 3,137,118 - -0.2 3,137,118 - +1.2 3,137,118 - -0.0 3,137,118 - -0.4 3,137,118 - -0.1 3,137,118 - -0.2 3,137,136 - -1.8 3,137,136 - -1.1 3,137,267 + +0.7 3,137,268 - -1.5 3,137,506 + ABIE41_RS15340 0.65 +0.9 3,137,727 + -0.8 3,138,055 + ABIE41_RS15345 0.21 +0.1 3,138,056 - ABIE41_RS15345 0.21 -1.2 3,138,056 - ABIE41_RS15345 0.21 +0.5 3,138,056 - ABIE41_RS15345 0.21 -0.3 3,138,202 + ABIE41_RS15345 0.35 +2.6 3,138,203 - ABIE41_RS15345 0.35 -1.8 3,138,203 - ABIE41_RS15345 0.35 +0.1
Or see this region's nucleotide sequence