Experiment: R2A with 1-octen-3-ol 0.542729333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS11365 and ABIE41_RS11370 are separated by 65 nucleotides ABIE41_RS11370 and ABIE41_RS11375 are separated by 98 nucleotides ABIE41_RS11375 and ABIE41_RS11380 overlap by 4 nucleotides ABIE41_RS11380 and ABIE41_RS11385 are separated by 78 nucleotides
ABIE41_RS11365: ABIE41_RS11365 - pyrroline-5-carboxylate reductase, at 2,333,960 to 2,334,775
_RS11365
ABIE41_RS11370: ABIE41_RS11370 - TetR/AcrR family transcriptional regulator, at 2,334,841 to 2,335,590
_RS11370
ABIE41_RS11375: ABIE41_RS11375 - methyl-accepting chemotaxis protein, at 2,335,689 to 2,337,686
_RS11375
ABIE41_RS11380: ABIE41_RS11380 - tRNA-binding protein, at 2,337,683 to 2,338,051
_RS11380
ABIE41_RS11385: ABIE41_RS11385 - histidine kinase dimerization/phosphoacceptor domain -containing protein, at 2,338,130 to 2,339,344
_RS11385
Position (kb)
2335
2336
2337
2338 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2334.805 kb on + strand at 2334.805 kb on + strand at 2334.805 kb on + strand at 2334.805 kb on + strand at 2334.805 kb on + strand at 2334.805 kb on + strand at 2334.805 kb on + strand at 2334.805 kb on + strand at 2334.805 kb on + strand at 2334.805 kb on + strand at 2334.806 kb on - strand at 2334.806 kb on - strand at 2334.806 kb on - strand at 2334.806 kb on - strand at 2334.806 kb on - strand at 2334.806 kb on - strand at 2334.806 kb on - strand at 2335.013 kb on + strand, within ABIE41_RS11370 at 2335.171 kb on + strand, within ABIE41_RS11370 at 2335.171 kb on + strand, within ABIE41_RS11370 at 2335.171 kb on + strand, within ABIE41_RS11370 at 2335.172 kb on - strand, within ABIE41_RS11370 at 2335.265 kb on - strand, within ABIE41_RS11370 at 2335.265 kb on - strand, within ABIE41_RS11370 at 2335.638 kb on - strand at 2335.686 kb on + strand at 2335.687 kb on - strand at 2335.687 kb on - strand at 2335.687 kb on - strand at 2335.794 kb on + strand at 2335.794 kb on + strand at 2335.794 kb on + strand at 2335.794 kb on + strand at 2335.794 kb on + strand at 2335.795 kb on - strand at 2335.795 kb on - strand at 2335.795 kb on - strand at 2335.795 kb on - strand at 2335.795 kb on - strand at 2335.975 kb on - strand, within ABIE41_RS11375 at 2335.975 kb on - strand, within ABIE41_RS11375 at 2335.975 kb on - strand, within ABIE41_RS11375 at 2335.975 kb on - strand, within ABIE41_RS11375 at 2335.975 kb on - strand, within ABIE41_RS11375 at 2335.977 kb on + strand, within ABIE41_RS11375 at 2335.978 kb on - strand, within ABIE41_RS11375 at 2335.978 kb on - strand, within ABIE41_RS11375 at 2335.978 kb on - strand, within ABIE41_RS11375 at 2336.121 kb on + strand, within ABIE41_RS11375 at 2336.122 kb on - strand, within ABIE41_RS11375 at 2336.131 kb on - strand, within ABIE41_RS11375 at 2336.178 kb on + strand, within ABIE41_RS11375 at 2336.178 kb on + strand, within ABIE41_RS11375 at 2336.178 kb on + strand, within ABIE41_RS11375 at 2336.178 kb on + strand, within ABIE41_RS11375 at 2336.178 kb on + strand, within ABIE41_RS11375 at 2336.179 kb on - strand, within ABIE41_RS11375 at 2336.179 kb on - strand, within ABIE41_RS11375 at 2336.665 kb on - strand, within ABIE41_RS11375 at 2336.694 kb on + strand, within ABIE41_RS11375 at 2336.768 kb on + strand, within ABIE41_RS11375 at 2336.769 kb on - strand, within ABIE41_RS11375 at 2336.769 kb on - strand, within ABIE41_RS11375 at 2337.816 kb on + strand, within ABIE41_RS11380 at 2337.816 kb on + strand, within ABIE41_RS11380 at 2337.878 kb on + strand, within ABIE41_RS11380 at 2337.878 kb on + strand, within ABIE41_RS11380 at 2337.879 kb on - strand, within ABIE41_RS11380 at 2337.879 kb on - strand, within ABIE41_RS11380 at 2338.046 kb on + strand at 2338.046 kb on + strand at 2338.046 kb on + strand at 2338.046 kb on + strand at 2338.046 kb on + strand at 2338.046 kb on + strand at 2338.047 kb on - strand at 2338.047 kb on - strand at 2338.047 kb on - strand at 2338.047 kb on - strand at 2338.047 kb on - strand at 2338.047 kb on - strand at 2338.096 kb on - strand at 2338.193 kb on + strand at 2338.313 kb on + strand, within ABIE41_RS11385 at 2338.520 kb on + strand, within ABIE41_RS11385 at 2338.643 kb on - strand, within ABIE41_RS11385 at 2338.647 kb on - strand, within ABIE41_RS11385 at 2338.647 kb on - strand, within ABIE41_RS11385
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.542729333333333 mM remove 2,334,805 + +0.4 2,334,805 + +0.4 2,334,805 + -0.0 2,334,805 + +1.1 2,334,805 + +1.7 2,334,805 + +0.8 2,334,805 + +1.9 2,334,805 + +0.2 2,334,805 + +0.2 2,334,805 + -0.5 2,334,806 - -0.8 2,334,806 - -2.5 2,334,806 - +0.7 2,334,806 - -0.1 2,334,806 - -1.1 2,334,806 - +0.3 2,334,806 - +0.3 2,335,013 + ABIE41_RS11370 0.23 -0.0 2,335,171 + ABIE41_RS11370 0.44 +0.9 2,335,171 + ABIE41_RS11370 0.44 -1.3 2,335,171 + ABIE41_RS11370 0.44 -0.2 2,335,172 - ABIE41_RS11370 0.44 +0.2 2,335,265 - ABIE41_RS11370 0.57 -2.4 2,335,265 - ABIE41_RS11370 0.57 +1.3 2,335,638 - -0.1 2,335,686 + +0.5 2,335,687 - -1.3 2,335,687 - -0.4 2,335,687 - -0.5 2,335,794 + +1.0 2,335,794 + +1.4 2,335,794 + -1.5 2,335,794 + -1.3 2,335,794 + +0.5 2,335,795 - +0.2 2,335,795 - +0.4 2,335,795 - -1.8 2,335,795 - +0.4 2,335,795 - +0.3 2,335,975 - ABIE41_RS11375 0.14 -0.2 2,335,975 - ABIE41_RS11375 0.14 +0.1 2,335,975 - ABIE41_RS11375 0.14 +0.6 2,335,975 - ABIE41_RS11375 0.14 +0.1 2,335,975 - ABIE41_RS11375 0.14 +0.5 2,335,977 + ABIE41_RS11375 0.14 +0.5 2,335,978 - ABIE41_RS11375 0.14 +0.5 2,335,978 - ABIE41_RS11375 0.14 -0.3 2,335,978 - ABIE41_RS11375 0.14 -3.6 2,336,121 + ABIE41_RS11375 0.22 +0.3 2,336,122 - ABIE41_RS11375 0.22 -1.5 2,336,131 - ABIE41_RS11375 0.22 -0.2 2,336,178 + ABIE41_RS11375 0.24 +2.1 2,336,178 + ABIE41_RS11375 0.24 -0.5 2,336,178 + ABIE41_RS11375 0.24 +0.9 2,336,178 + ABIE41_RS11375 0.24 +0.6 2,336,178 + ABIE41_RS11375 0.24 +0.8 2,336,179 - ABIE41_RS11375 0.25 -0.9 2,336,179 - ABIE41_RS11375 0.25 -0.0 2,336,665 - ABIE41_RS11375 0.49 -0.6 2,336,694 + ABIE41_RS11375 0.50 +0.3 2,336,768 + ABIE41_RS11375 0.54 -0.5 2,336,769 - ABIE41_RS11375 0.54 -0.2 2,336,769 - ABIE41_RS11375 0.54 +1.7 2,337,816 + ABIE41_RS11380 0.36 -0.7 2,337,816 + ABIE41_RS11380 0.36 +0.7 2,337,878 + ABIE41_RS11380 0.53 -0.6 2,337,878 + ABIE41_RS11380 0.53 -0.1 2,337,879 - ABIE41_RS11380 0.53 -0.4 2,337,879 - ABIE41_RS11380 0.53 +0.0 2,338,046 + +0.3 2,338,046 + -1.3 2,338,046 + +0.5 2,338,046 + +0.4 2,338,046 + +0.5 2,338,046 + -2.1 2,338,047 - -2.2 2,338,047 - +0.1 2,338,047 - -0.1 2,338,047 - -0.1 2,338,047 - -0.1 2,338,047 - -0.4 2,338,096 - +0.2 2,338,193 + -0.9 2,338,313 + ABIE41_RS11385 0.15 -0.4 2,338,520 + ABIE41_RS11385 0.32 -2.5 2,338,643 - ABIE41_RS11385 0.42 -1.0 2,338,647 - ABIE41_RS11385 0.43 -1.4 2,338,647 - ABIE41_RS11385 0.43 +0.2
Or see this region's nucleotide sequence