Experiment: R2A with 1-octen-3-ol 0.542729333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS09720 and ABIE41_RS09725 overlap by 4 nucleotides ABIE41_RS09725 and ABIE41_RS09730 are separated by 96 nucleotides ABIE41_RS09730 and ABIE41_RS09735 are separated by 22 nucleotides
ABIE41_RS09720: ABIE41_RS09720 - ABC transporter permease subunit, at 2,009,202 to 2,010,035
_RS09720
ABIE41_RS09725: ABIE41_RS09725 - ABC transporter permease subunit, at 2,010,032 to 2,010,848
_RS09725
ABIE41_RS09730: ABIE41_RS09730 - O-antigen ligase, at 2,010,945 to 2,012,267
_RS09730
ABIE41_RS09735: ABIE41_RS09735 - acyltransferase, at 2,012,290 to 2,013,405
_RS09735
Position (kb)
2010
2011
2012
2013 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2009.967 kb on + strand at 2009.967 kb on + strand at 2009.967 kb on + strand at 2009.967 kb on + strand at 2009.968 kb on - strand at 2009.968 kb on - strand at 2009.968 kb on - strand at 2009.968 kb on - strand at 2009.968 kb on - strand at 2009.968 kb on - strand at 2009.998 kb on - strand at 2010.152 kb on + strand, within ABIE41_RS09725 at 2010.153 kb on - strand, within ABIE41_RS09725 at 2010.153 kb on - strand, within ABIE41_RS09725 at 2010.161 kb on + strand, within ABIE41_RS09725 at 2010.161 kb on + strand, within ABIE41_RS09725 at 2010.161 kb on + strand, within ABIE41_RS09725 at 2010.161 kb on + strand, within ABIE41_RS09725 at 2010.162 kb on - strand, within ABIE41_RS09725 at 2010.162 kb on - strand, within ABIE41_RS09725 at 2010.162 kb on - strand, within ABIE41_RS09725 at 2010.162 kb on - strand, within ABIE41_RS09725 at 2010.179 kb on + strand, within ABIE41_RS09725 at 2010.392 kb on + strand, within ABIE41_RS09725 at 2010.393 kb on - strand, within ABIE41_RS09725 at 2010.393 kb on - strand, within ABIE41_RS09725 at 2010.393 kb on - strand, within ABIE41_RS09725 at 2010.393 kb on - strand, within ABIE41_RS09725 at 2010.551 kb on + strand, within ABIE41_RS09725 at 2010.660 kb on - strand, within ABIE41_RS09725 at 2010.715 kb on - strand, within ABIE41_RS09725 at 2010.738 kb on + strand, within ABIE41_RS09725 at 2010.738 kb on + strand, within ABIE41_RS09725 at 2010.738 kb on + strand, within ABIE41_RS09725 at 2010.738 kb on + strand, within ABIE41_RS09725 at 2010.738 kb on + strand, within ABIE41_RS09725 at 2010.739 kb on - strand, within ABIE41_RS09725 at 2010.846 kb on + strand at 2010.847 kb on - strand at 2010.847 kb on - strand at 2010.885 kb on + strand at 2010.885 kb on + strand at 2010.886 kb on - strand at 2010.886 kb on - strand at 2010.886 kb on - strand at 2011.143 kb on + strand, within ABIE41_RS09730 at 2011.144 kb on - strand, within ABIE41_RS09730 at 2011.144 kb on - strand, within ABIE41_RS09730 at 2011.164 kb on + strand, within ABIE41_RS09730 at 2011.165 kb on - strand, within ABIE41_RS09730 at 2011.416 kb on + strand, within ABIE41_RS09730 at 2011.416 kb on + strand, within ABIE41_RS09730 at 2011.417 kb on - strand, within ABIE41_RS09730 at 2011.417 kb on - strand, within ABIE41_RS09730 at 2011.518 kb on + strand, within ABIE41_RS09730 at 2011.519 kb on - strand, within ABIE41_RS09730 at 2011.560 kb on + strand, within ABIE41_RS09730 at 2011.560 kb on + strand, within ABIE41_RS09730 at 2011.560 kb on + strand, within ABIE41_RS09730 at 2011.560 kb on + strand, within ABIE41_RS09730 at 2011.560 kb on + strand, within ABIE41_RS09730 at 2011.560 kb on + strand, within ABIE41_RS09730 at 2011.560 kb on + strand, within ABIE41_RS09730 at 2011.560 kb on + strand, within ABIE41_RS09730 at 2011.560 kb on + strand, within ABIE41_RS09730 at 2011.560 kb on + strand, within ABIE41_RS09730 at 2011.560 kb on + strand, within ABIE41_RS09730 at 2011.560 kb on + strand, within ABIE41_RS09730 at 2011.560 kb on + strand, within ABIE41_RS09730 at 2011.561 kb on - strand, within ABIE41_RS09730 at 2011.561 kb on - strand, within ABIE41_RS09730 at 2011.561 kb on - strand, within ABIE41_RS09730 at 2011.561 kb on - strand, within ABIE41_RS09730 at 2011.561 kb on - strand, within ABIE41_RS09730 at 2011.561 kb on - strand, within ABIE41_RS09730 at 2011.561 kb on - strand, within ABIE41_RS09730 at 2011.899 kb on + strand, within ABIE41_RS09730 at 2011.899 kb on + strand, within ABIE41_RS09730 at 2011.900 kb on - strand, within ABIE41_RS09730 at 2011.900 kb on - strand, within ABIE41_RS09730 at 2011.995 kb on + strand, within ABIE41_RS09730 at 2011.995 kb on + strand, within ABIE41_RS09730 at 2011.995 kb on + strand, within ABIE41_RS09730 at 2011.996 kb on - strand, within ABIE41_RS09730 at 2011.996 kb on - strand, within ABIE41_RS09730 at 2011.996 kb on - strand, within ABIE41_RS09730 at 2011.996 kb on - strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.046 kb on + strand, within ABIE41_RS09730 at 2012.047 kb on - strand, within ABIE41_RS09730 at 2012.047 kb on - strand, within ABIE41_RS09730 at 2012.047 kb on - strand, within ABIE41_RS09730 at 2012.047 kb on - strand, within ABIE41_RS09730 at 2012.047 kb on - strand, within ABIE41_RS09730 at 2012.047 kb on - strand, within ABIE41_RS09730 at 2012.047 kb on - strand, within ABIE41_RS09730 at 2012.076 kb on + strand, within ABIE41_RS09730 at 2012.076 kb on + strand, within ABIE41_RS09730 at 2012.076 kb on + strand, within ABIE41_RS09730 at 2012.076 kb on + strand, within ABIE41_RS09730 at 2012.076 kb on + strand, within ABIE41_RS09730 at 2012.076 kb on + strand, within ABIE41_RS09730 at 2012.077 kb on - strand, within ABIE41_RS09730 at 2012.118 kb on + strand, within ABIE41_RS09730 at 2012.151 kb on + strand at 2012.151 kb on + strand at 2012.151 kb on + strand at 2012.152 kb on - strand at 2012.152 kb on - strand at 2012.152 kb on - strand at 2012.152 kb on - strand at 2012.384 kb on + strand at 2012.384 kb on + strand at 2012.384 kb on + strand at 2012.384 kb on + strand at 2012.384 kb on + strand at 2012.385 kb on - strand at 2012.505 kb on - strand, within ABIE41_RS09735 at 2012.505 kb on - strand, within ABIE41_RS09735 at 2012.505 kb on - strand, within ABIE41_RS09735 at 2012.505 kb on - strand, within ABIE41_RS09735 at 2012.510 kb on + strand, within ABIE41_RS09735 at 2012.510 kb on + strand, within ABIE41_RS09735 at 2012.510 kb on + strand, within ABIE41_RS09735 at 2012.510 kb on + strand, within ABIE41_RS09735 at 2012.510 kb on + strand, within ABIE41_RS09735 at 2012.511 kb on - strand, within ABIE41_RS09735 at 2012.511 kb on - strand, within ABIE41_RS09735 at 2012.511 kb on - strand, within ABIE41_RS09735 at 2012.576 kb on + strand, within ABIE41_RS09735 at 2012.576 kb on + strand, within ABIE41_RS09735 at 2012.576 kb on + strand, within ABIE41_RS09735 at 2012.576 kb on + strand, within ABIE41_RS09735 at 2012.576 kb on + strand, within ABIE41_RS09735 at 2012.576 kb on + strand, within ABIE41_RS09735 at 2012.577 kb on - strand, within ABIE41_RS09735 at 2012.577 kb on - strand, within ABIE41_RS09735 at 2012.577 kb on - strand, within ABIE41_RS09735 at 2012.577 kb on - strand, within ABIE41_RS09735 at 2012.577 kb on - strand, within ABIE41_RS09735 at 2012.589 kb on - strand, within ABIE41_RS09735 at 2012.708 kb on + strand, within ABIE41_RS09735 at 2012.708 kb on + strand, within ABIE41_RS09735 at 2012.708 kb on + strand, within ABIE41_RS09735 at 2012.708 kb on + strand, within ABIE41_RS09735 at 2012.709 kb on - strand, within ABIE41_RS09735 at 2012.709 kb on - strand, within ABIE41_RS09735 at 2012.709 kb on - strand, within ABIE41_RS09735 at 2012.756 kb on + strand, within ABIE41_RS09735 at 2012.756 kb on + strand, within ABIE41_RS09735 at 2012.756 kb on + strand, within ABIE41_RS09735 at 2012.757 kb on - strand, within ABIE41_RS09735 at 2012.757 kb on - strand, within ABIE41_RS09735 at 2012.757 kb on - strand, within ABIE41_RS09735 at 2012.757 kb on - strand, within ABIE41_RS09735 at 2012.757 kb on - strand, within ABIE41_RS09735 at 2012.790 kb on - strand, within ABIE41_RS09735 at 2012.891 kb on + strand, within ABIE41_RS09735 at 2012.891 kb on + strand, within ABIE41_RS09735 at 2012.891 kb on + strand, within ABIE41_RS09735 at 2012.891 kb on + strand, within ABIE41_RS09735 at 2012.891 kb on + strand, within ABIE41_RS09735 at 2012.891 kb on + strand, within ABIE41_RS09735 at 2012.892 kb on - strand, within ABIE41_RS09735 at 2012.897 kb on + strand, within ABIE41_RS09735 at 2012.897 kb on + strand, within ABIE41_RS09735 at 2013.009 kb on - strand, within ABIE41_RS09735 at 2013.035 kb on + strand, within ABIE41_RS09735 at 2013.035 kb on + strand, within ABIE41_RS09735 at 2013.050 kb on + strand, within ABIE41_RS09735 at 2013.050 kb on + strand, within ABIE41_RS09735 at 2013.050 kb on + strand, within ABIE41_RS09735 at 2013.050 kb on + strand, within ABIE41_RS09735 at 2013.050 kb on + strand, within ABIE41_RS09735 at 2013.051 kb on - strand, within ABIE41_RS09735 at 2013.051 kb on - strand, within ABIE41_RS09735 at 2013.051 kb on - strand, within ABIE41_RS09735
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.542729333333333 mM remove 2,009,967 + -0.3 2,009,967 + -0.2 2,009,967 + -0.5 2,009,967 + +0.6 2,009,968 - -0.5 2,009,968 - +0.3 2,009,968 - -0.9 2,009,968 - -1.5 2,009,968 - +0.1 2,009,968 - +0.7 2,009,998 - +0.8 2,010,152 + ABIE41_RS09725 0.15 -2.0 2,010,153 - ABIE41_RS09725 0.15 +1.1 2,010,153 - ABIE41_RS09725 0.15 +0.3 2,010,161 + ABIE41_RS09725 0.16 -0.3 2,010,161 + ABIE41_RS09725 0.16 -1.9 2,010,161 + ABIE41_RS09725 0.16 -0.8 2,010,161 + ABIE41_RS09725 0.16 -0.3 2,010,162 - ABIE41_RS09725 0.16 +0.0 2,010,162 - ABIE41_RS09725 0.16 -0.4 2,010,162 - ABIE41_RS09725 0.16 -0.8 2,010,162 - ABIE41_RS09725 0.16 +1.4 2,010,179 + ABIE41_RS09725 0.18 -1.8 2,010,392 + ABIE41_RS09725 0.44 -0.7 2,010,393 - ABIE41_RS09725 0.44 +1.1 2,010,393 - ABIE41_RS09725 0.44 -0.4 2,010,393 - ABIE41_RS09725 0.44 -1.3 2,010,393 - ABIE41_RS09725 0.44 +0.6 2,010,551 + ABIE41_RS09725 0.64 -1.9 2,010,660 - ABIE41_RS09725 0.77 +3.1 2,010,715 - ABIE41_RS09725 0.84 +1.0 2,010,738 + ABIE41_RS09725 0.86 +0.5 2,010,738 + ABIE41_RS09725 0.86 +0.3 2,010,738 + ABIE41_RS09725 0.86 +0.5 2,010,738 + ABIE41_RS09725 0.86 +1.8 2,010,738 + ABIE41_RS09725 0.86 +1.1 2,010,739 - ABIE41_RS09725 0.87 -0.5 2,010,846 + -0.5 2,010,847 - -0.1 2,010,847 - -0.4 2,010,885 + -1.0 2,010,885 + -0.2 2,010,886 - -1.3 2,010,886 - -1.7 2,010,886 - -1.6 2,011,143 + ABIE41_RS09730 0.15 -1.8 2,011,144 - ABIE41_RS09730 0.15 -1.2 2,011,144 - ABIE41_RS09730 0.15 -0.3 2,011,164 + ABIE41_RS09730 0.17 -2.3 2,011,165 - ABIE41_RS09730 0.17 +0.1 2,011,416 + ABIE41_RS09730 0.36 -2.3 2,011,416 + ABIE41_RS09730 0.36 +0.1 2,011,417 - ABIE41_RS09730 0.36 -0.5 2,011,417 - ABIE41_RS09730 0.36 -0.5 2,011,518 + ABIE41_RS09730 0.43 -0.7 2,011,519 - ABIE41_RS09730 0.43 -1.3 2,011,560 + ABIE41_RS09730 0.46 +0.4 2,011,560 + ABIE41_RS09730 0.46 -0.6 2,011,560 + ABIE41_RS09730 0.46 -0.7 2,011,560 + ABIE41_RS09730 0.46 +0.4 2,011,560 + ABIE41_RS09730 0.46 +0.6 2,011,560 + ABIE41_RS09730 0.46 -1.5 2,011,560 + ABIE41_RS09730 0.46 -0.8 2,011,560 + ABIE41_RS09730 0.46 +0.3 2,011,560 + ABIE41_RS09730 0.46 +1.1 2,011,560 + ABIE41_RS09730 0.46 +0.0 2,011,560 + ABIE41_RS09730 0.46 +0.8 2,011,560 + ABIE41_RS09730 0.46 +0.6 2,011,560 + ABIE41_RS09730 0.46 -0.5 2,011,561 - ABIE41_RS09730 0.47 +1.4 2,011,561 - ABIE41_RS09730 0.47 +0.1 2,011,561 - ABIE41_RS09730 0.47 +0.2 2,011,561 - ABIE41_RS09730 0.47 -1.5 2,011,561 - ABIE41_RS09730 0.47 +0.0 2,011,561 - ABIE41_RS09730 0.47 -1.6 2,011,561 - ABIE41_RS09730 0.47 -0.6 2,011,899 + ABIE41_RS09730 0.72 +0.4 2,011,899 + ABIE41_RS09730 0.72 -0.2 2,011,900 - ABIE41_RS09730 0.72 +0.2 2,011,900 - ABIE41_RS09730 0.72 -0.2 2,011,995 + ABIE41_RS09730 0.79 -2.4 2,011,995 + ABIE41_RS09730 0.79 -0.0 2,011,995 + ABIE41_RS09730 0.79 +0.1 2,011,996 - ABIE41_RS09730 0.79 -0.0 2,011,996 - ABIE41_RS09730 0.79 -1.3 2,011,996 - ABIE41_RS09730 0.79 -0.4 2,011,996 - ABIE41_RS09730 0.79 +0.5 2,012,046 + ABIE41_RS09730 0.83 +1.4 2,012,046 + ABIE41_RS09730 0.83 -1.2 2,012,046 + ABIE41_RS09730 0.83 -0.7 2,012,046 + ABIE41_RS09730 0.83 -0.9 2,012,046 + ABIE41_RS09730 0.83 +2.0 2,012,046 + ABIE41_RS09730 0.83 -1.3 2,012,046 + ABIE41_RS09730 0.83 -1.2 2,012,046 + ABIE41_RS09730 0.83 +0.8 2,012,046 + ABIE41_RS09730 0.83 -1.7 2,012,046 + ABIE41_RS09730 0.83 -0.6 2,012,046 + ABIE41_RS09730 0.83 +0.7 2,012,046 + ABIE41_RS09730 0.83 +0.1 2,012,046 + ABIE41_RS09730 0.83 +0.1 2,012,046 + ABIE41_RS09730 0.83 -0.4 2,012,047 - ABIE41_RS09730 0.83 -0.9 2,012,047 - ABIE41_RS09730 0.83 -0.5 2,012,047 - ABIE41_RS09730 0.83 +0.3 2,012,047 - ABIE41_RS09730 0.83 -0.6 2,012,047 - ABIE41_RS09730 0.83 +0.2 2,012,047 - ABIE41_RS09730 0.83 +0.7 2,012,047 - ABIE41_RS09730 0.83 +0.2 2,012,076 + ABIE41_RS09730 0.85 +0.3 2,012,076 + ABIE41_RS09730 0.85 +2.1 2,012,076 + ABIE41_RS09730 0.85 +0.1 2,012,076 + ABIE41_RS09730 0.85 +0.1 2,012,076 + ABIE41_RS09730 0.85 +0.0 2,012,076 + ABIE41_RS09730 0.85 -1.1 2,012,077 - ABIE41_RS09730 0.86 +1.3 2,012,118 + ABIE41_RS09730 0.89 -1.0 2,012,151 + +0.1 2,012,151 + +3.6 2,012,151 + -0.5 2,012,152 - -3.5 2,012,152 - -0.1 2,012,152 - +0.1 2,012,152 - -0.6 2,012,384 + +0.4 2,012,384 + +0.7 2,012,384 + +0.8 2,012,384 + -2.3 2,012,384 + -0.7 2,012,385 - +1.8 2,012,505 - ABIE41_RS09735 0.19 +0.5 2,012,505 - ABIE41_RS09735 0.19 -0.3 2,012,505 - ABIE41_RS09735 0.19 -1.6 2,012,505 - ABIE41_RS09735 0.19 +0.2 2,012,510 + ABIE41_RS09735 0.20 -1.1 2,012,510 + ABIE41_RS09735 0.20 +0.0 2,012,510 + ABIE41_RS09735 0.20 +0.2 2,012,510 + ABIE41_RS09735 0.20 -1.5 2,012,510 + ABIE41_RS09735 0.20 -0.8 2,012,511 - ABIE41_RS09735 0.20 -0.0 2,012,511 - ABIE41_RS09735 0.20 -0.2 2,012,511 - ABIE41_RS09735 0.20 +2.5 2,012,576 + ABIE41_RS09735 0.26 +1.5 2,012,576 + ABIE41_RS09735 0.26 -1.3 2,012,576 + ABIE41_RS09735 0.26 +0.2 2,012,576 + ABIE41_RS09735 0.26 -1.0 2,012,576 + ABIE41_RS09735 0.26 +1.8 2,012,576 + ABIE41_RS09735 0.26 +0.6 2,012,577 - ABIE41_RS09735 0.26 -1.1 2,012,577 - ABIE41_RS09735 0.26 -0.5 2,012,577 - ABIE41_RS09735 0.26 +0.7 2,012,577 - ABIE41_RS09735 0.26 +0.5 2,012,577 - ABIE41_RS09735 0.26 -1.3 2,012,589 - ABIE41_RS09735 0.27 -0.6 2,012,708 + ABIE41_RS09735 0.37 -0.5 2,012,708 + ABIE41_RS09735 0.37 +0.2 2,012,708 + ABIE41_RS09735 0.37 -0.8 2,012,708 + ABIE41_RS09735 0.37 +0.5 2,012,709 - ABIE41_RS09735 0.38 +0.5 2,012,709 - ABIE41_RS09735 0.38 +0.2 2,012,709 - ABIE41_RS09735 0.38 -0.3 2,012,756 + ABIE41_RS09735 0.42 -0.5 2,012,756 + ABIE41_RS09735 0.42 +1.8 2,012,756 + ABIE41_RS09735 0.42 +0.5 2,012,757 - ABIE41_RS09735 0.42 +1.2 2,012,757 - ABIE41_RS09735 0.42 -2.8 2,012,757 - ABIE41_RS09735 0.42 -0.4 2,012,757 - ABIE41_RS09735 0.42 +0.1 2,012,757 - ABIE41_RS09735 0.42 +0.8 2,012,790 - ABIE41_RS09735 0.45 -0.7 2,012,891 + ABIE41_RS09735 0.54 -1.2 2,012,891 + ABIE41_RS09735 0.54 -0.5 2,012,891 + ABIE41_RS09735 0.54 +0.1 2,012,891 + ABIE41_RS09735 0.54 +1.2 2,012,891 + ABIE41_RS09735 0.54 +0.8 2,012,891 + ABIE41_RS09735 0.54 -0.0 2,012,892 - ABIE41_RS09735 0.54 +1.2 2,012,897 + ABIE41_RS09735 0.54 -0.1 2,012,897 + ABIE41_RS09735 0.54 +0.5 2,013,009 - ABIE41_RS09735 0.64 +0.0 2,013,035 + ABIE41_RS09735 0.67 +0.0 2,013,035 + ABIE41_RS09735 0.67 -0.4 2,013,050 + ABIE41_RS09735 0.68 +0.2 2,013,050 + ABIE41_RS09735 0.68 +0.4 2,013,050 + ABIE41_RS09735 0.68 +0.2 2,013,050 + ABIE41_RS09735 0.68 +0.2 2,013,050 + ABIE41_RS09735 0.68 -1.0 2,013,051 - ABIE41_RS09735 0.68 +0.1 2,013,051 - ABIE41_RS09735 0.68 -0.2 2,013,051 - ABIE41_RS09735 0.68 -1.8
Or see this region's nucleotide sequence