Experiment: R2A with 1-octen-3-ol 0.542729333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS04985 and ABIE41_RS04990 are separated by 13 nucleotides ABIE41_RS04990 and ABIE41_RS04995 overlap by 4 nucleotides ABIE41_RS04995 and ABIE41_RS05000 are separated by 116 nucleotides ABIE41_RS05000 and ABIE41_RS05005 are separated by 4 nucleotides ABIE41_RS05005 and ABIE41_RS05010 are separated by 80 nucleotides ABIE41_RS05010 and ABIE41_RS05015 are separated by 142 nucleotides
ABIE41_RS04985: ABIE41_RS04985 - heme ABC transporter permease, at 1,038,004 to 1,038,765
_RS04985
ABIE41_RS04990: ABIE41_RS04990 - heme exporter protein CcmD, at 1,038,779 to 1,038,952
_RS04990
ABIE41_RS04995: ABIE41_RS04995 - DsbE family thiol:disulfide interchange protein, at 1,038,949 to 1,039,554
_RS04995
ABIE41_RS05000: ABIE41_RS05000 - phosphoribosylformylglycinamidine synthase subunit PurQ, at 1,039,671 to 1,040,366
_RS05000
ABIE41_RS05005: ABIE41_RS05005 - phosphoribosylformylglycinamidine synthase subunit PurS, at 1,040,371 to 1,040,613
_RS05005
ABIE41_RS05010: ABIE41_RS05010 - hypothetical protein, at 1,040,694 to 1,041,200
_RS05010
ABIE41_RS05015: ABIE41_RS05015 - phosphoribosylaminoimidazolesuccinocarboxamide synthase, at 1,041,343 to 1,042,137
_RS05015
Position (kb)
1039
1040
1041 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1039.014 kb on - strand, within ABIE41_RS04995 at 1039.795 kb on + strand, within ABIE41_RS05000 at 1039.795 kb on + strand, within ABIE41_RS05000 at 1039.796 kb on - strand, within ABIE41_RS05000 at 1039.796 kb on - strand, within ABIE41_RS05000 at 1039.796 kb on - strand, within ABIE41_RS05000 at 1039.796 kb on - strand, within ABIE41_RS05000 at 1039.867 kb on + strand, within ABIE41_RS05000 at 1039.927 kb on + strand, within ABIE41_RS05000 at 1039.927 kb on + strand, within ABIE41_RS05000 at 1039.927 kb on + strand, within ABIE41_RS05000 at 1039.927 kb on + strand, within ABIE41_RS05000 at 1039.927 kb on + strand, within ABIE41_RS05000 at 1039.927 kb on + strand, within ABIE41_RS05000 at 1039.928 kb on - strand, within ABIE41_RS05000 at 1039.928 kb on - strand, within ABIE41_RS05000 at 1039.978 kb on + strand, within ABIE41_RS05000 at 1039.978 kb on + strand, within ABIE41_RS05000 at 1039.988 kb on + strand, within ABIE41_RS05000 at 1039.990 kb on + strand, within ABIE41_RS05000 at 1039.991 kb on - strand, within ABIE41_RS05000 at 1040.125 kb on + strand, within ABIE41_RS05000 at 1040.126 kb on - strand, within ABIE41_RS05000 at 1040.200 kb on + strand, within ABIE41_RS05000 at 1040.200 kb on + strand, within ABIE41_RS05000 at 1040.393 kb on + strand at 1040.393 kb on + strand at 1040.393 kb on + strand at 1040.393 kb on + strand at 1040.393 kb on + strand at 1040.393 kb on + strand at 1040.393 kb on + strand at 1040.394 kb on - strand at 1040.394 kb on - strand at 1040.394 kb on - strand at 1041.291 kb on + strand at 1041.291 kb on + strand at 1041.291 kb on + strand at 1041.292 kb on - strand at 1041.292 kb on - strand at 1041.344 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.542729333333333 mM remove 1,039,014 - ABIE41_RS04995 0.11 -2.8 1,039,795 + ABIE41_RS05000 0.18 -3.1 1,039,795 + ABIE41_RS05000 0.18 -1.8 1,039,796 - ABIE41_RS05000 0.18 -0.9 1,039,796 - ABIE41_RS05000 0.18 -1.8 1,039,796 - ABIE41_RS05000 0.18 -0.5 1,039,796 - ABIE41_RS05000 0.18 -3.5 1,039,867 + ABIE41_RS05000 0.28 -1.2 1,039,927 + ABIE41_RS05000 0.37 -0.9 1,039,927 + ABIE41_RS05000 0.37 -2.1 1,039,927 + ABIE41_RS05000 0.37 -0.9 1,039,927 + ABIE41_RS05000 0.37 -0.9 1,039,927 + ABIE41_RS05000 0.37 -1.1 1,039,927 + ABIE41_RS05000 0.37 -2.7 1,039,928 - ABIE41_RS05000 0.37 +1.2 1,039,928 - ABIE41_RS05000 0.37 -0.0 1,039,978 + ABIE41_RS05000 0.44 +0.6 1,039,978 + ABIE41_RS05000 0.44 +1.9 1,039,988 + ABIE41_RS05000 0.46 -1.0 1,039,990 + ABIE41_RS05000 0.46 +0.8 1,039,991 - ABIE41_RS05000 0.46 -2.1 1,040,125 + ABIE41_RS05000 0.65 -1.0 1,040,126 - ABIE41_RS05000 0.65 -2.2 1,040,200 + ABIE41_RS05000 0.76 -0.8 1,040,200 + ABIE41_RS05000 0.76 -1.6 1,040,393 + +0.3 1,040,393 + +0.3 1,040,393 + -2.0 1,040,393 + -0.4 1,040,393 + -0.4 1,040,393 + -1.4 1,040,393 + +0.3 1,040,394 - +0.5 1,040,394 - -2.0 1,040,394 - -0.9 1,041,291 + -0.3 1,041,291 + +0.6 1,041,291 + -1.6 1,041,292 - +0.5 1,041,292 - -0.6 1,041,344 + -0.7
Or see this region's nucleotide sequence