Experiment: R2A with 1-octen-3-ol 0.542729333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS04810 and ABIE41_RS04815 are separated by 174 nucleotides ABIE41_RS04815 and ABIE41_RS04820 are separated by 87 nucleotides ABIE41_RS04820 and ABIE41_RS04825 are separated by 86 nucleotides ABIE41_RS04825 and ABIE41_RS04830 are separated by 2 nucleotides ABIE41_RS04830 and ABIE41_RS04835 are separated by 9 nucleotides
ABIE41_RS04810: ABIE41_RS04810 - ABC transporter ATP-binding protein, at 1,006,414 to 1,007,151
_RS04810
ABIE41_RS04815: ABIE41_RS04815 - arylesterase, at 1,007,326 to 1,007,934
_RS04815
ABIE41_RS04820: ABIE41_RS04820 - aldo/keto reductase, at 1,008,022 to 1,009,077
_RS04820
ABIE41_RS04825: ABIE41_RS04825 - RNA 2',3'-cyclic phosphodiesterase, at 1,009,164 to 1,009,742
_RS04825
ABIE41_RS04830: ABIE41_RS04830 - 4a-hydroxytetrahydrobiopterin dehydratase, at 1,009,745 to 1,010,035
_RS04830
ABIE41_RS04835: ABIE41_RS04835 - RNA pseudouridine synthase, at 1,010,045 to 1,010,704
_RS04835
Position (kb)
1008
1009
1010 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1007.085 kb on + strand at 1007.086 kb on - strand at 1007.086 kb on - strand at 1007.086 kb on - strand at 1007.086 kb on - strand at 1007.086 kb on - strand at 1007.414 kb on - strand, within ABIE41_RS04815 at 1007.414 kb on - strand, within ABIE41_RS04815 at 1007.652 kb on + strand, within ABIE41_RS04815 at 1007.652 kb on + strand, within ABIE41_RS04815 at 1007.652 kb on + strand, within ABIE41_RS04815 at 1007.653 kb on - strand, within ABIE41_RS04815 at 1007.653 kb on - strand, within ABIE41_RS04815 at 1007.653 kb on - strand, within ABIE41_RS04815 at 1007.653 kb on - strand, within ABIE41_RS04815 at 1007.653 kb on - strand, within ABIE41_RS04815 at 1007.698 kb on + strand, within ABIE41_RS04815 at 1007.698 kb on + strand, within ABIE41_RS04815 at 1007.698 kb on + strand, within ABIE41_RS04815 at 1007.698 kb on + strand, within ABIE41_RS04815 at 1007.698 kb on + strand, within ABIE41_RS04815 at 1007.699 kb on - strand, within ABIE41_RS04815 at 1007.699 kb on - strand, within ABIE41_RS04815 at 1007.699 kb on - strand, within ABIE41_RS04815 at 1007.699 kb on - strand, within ABIE41_RS04815 at 1007.699 kb on - strand, within ABIE41_RS04815 at 1007.699 kb on - strand, within ABIE41_RS04815 at 1007.725 kb on + strand, within ABIE41_RS04815 at 1007.725 kb on + strand, within ABIE41_RS04815 at 1007.725 kb on + strand, within ABIE41_RS04815 at 1007.725 kb on + strand, within ABIE41_RS04815 at 1007.725 kb on + strand, within ABIE41_RS04815 at 1007.725 kb on + strand, within ABIE41_RS04815 at 1007.726 kb on - strand, within ABIE41_RS04815 at 1007.726 kb on - strand, within ABIE41_RS04815 at 1007.726 kb on - strand, within ABIE41_RS04815 at 1007.726 kb on - strand, within ABIE41_RS04815 at 1007.726 kb on - strand, within ABIE41_RS04815 at 1007.726 kb on - strand, within ABIE41_RS04815 at 1007.726 kb on - strand, within ABIE41_RS04815 at 1007.726 kb on - strand, within ABIE41_RS04815 at 1007.726 kb on - strand, within ABIE41_RS04815 at 1007.749 kb on + strand, within ABIE41_RS04815 at 1007.750 kb on - strand, within ABIE41_RS04815 at 1007.750 kb on - strand, within ABIE41_RS04815 at 1007.750 kb on - strand, within ABIE41_RS04815 at 1007.750 kb on - strand, within ABIE41_RS04815 at 1007.750 kb on - strand, within ABIE41_RS04815 at 1007.786 kb on - strand, within ABIE41_RS04815 at 1007.786 kb on - strand, within ABIE41_RS04815 at 1007.893 kb on - strand at 1007.893 kb on - strand at 1008.017 kb on + strand at 1008.017 kb on + strand at 1008.018 kb on - strand at 1008.026 kb on - strand at 1008.028 kb on + strand at 1008.028 kb on + strand at 1008.029 kb on - strand at 1008.088 kb on + strand at 1008.088 kb on + strand at 1008.088 kb on + strand at 1008.089 kb on - strand at 1008.089 kb on - strand at 1008.089 kb on - strand at 1008.134 kb on - strand, within ABIE41_RS04820 at 1008.134 kb on - strand, within ABIE41_RS04820 at 1008.134 kb on - strand, within ABIE41_RS04820 at 1008.134 kb on - strand, within ABIE41_RS04820 at 1008.134 kb on - strand, within ABIE41_RS04820 at 1008.178 kb on + strand, within ABIE41_RS04820 at 1008.178 kb on + strand, within ABIE41_RS04820 at 1008.178 kb on + strand, within ABIE41_RS04820 at 1008.179 kb on - strand, within ABIE41_RS04820 at 1008.397 kb on + strand, within ABIE41_RS04820 at 1008.397 kb on + strand, within ABIE41_RS04820 at 1008.397 kb on + strand, within ABIE41_RS04820 at 1008.397 kb on + strand, within ABIE41_RS04820 at 1008.397 kb on + strand, within ABIE41_RS04820 at 1008.397 kb on + strand, within ABIE41_RS04820 at 1008.397 kb on + strand, within ABIE41_RS04820 at 1008.398 kb on - strand, within ABIE41_RS04820 at 1008.398 kb on - strand, within ABIE41_RS04820 at 1008.398 kb on - strand, within ABIE41_RS04820 at 1008.398 kb on - strand, within ABIE41_RS04820 at 1008.399 kb on + strand, within ABIE41_RS04820 at 1008.399 kb on + strand, within ABIE41_RS04820 at 1008.400 kb on - strand, within ABIE41_RS04820 at 1008.400 kb on - strand, within ABIE41_RS04820 at 1008.400 kb on - strand, within ABIE41_RS04820 at 1008.400 kb on - strand, within ABIE41_RS04820 at 1008.400 kb on - strand, within ABIE41_RS04820 at 1008.400 kb on - strand, within ABIE41_RS04820 at 1008.410 kb on - strand, within ABIE41_RS04820 at 1008.410 kb on - strand, within ABIE41_RS04820 at 1008.423 kb on - strand, within ABIE41_RS04820 at 1008.659 kb on - strand, within ABIE41_RS04820 at 1008.733 kb on + strand, within ABIE41_RS04820 at 1008.734 kb on - strand, within ABIE41_RS04820 at 1008.769 kb on + strand, within ABIE41_RS04820 at 1008.769 kb on + strand, within ABIE41_RS04820 at 1008.769 kb on + strand, within ABIE41_RS04820 at 1008.770 kb on - strand, within ABIE41_RS04820 at 1008.770 kb on - strand, within ABIE41_RS04820 at 1008.864 kb on + strand, within ABIE41_RS04820 at 1008.865 kb on + strand, within ABIE41_RS04820 at 1008.865 kb on + strand, within ABIE41_RS04820 at 1008.865 kb on + strand, within ABIE41_RS04820 at 1008.865 kb on + strand, within ABIE41_RS04820 at 1008.865 kb on + strand, within ABIE41_RS04820 at 1008.866 kb on - strand, within ABIE41_RS04820 at 1008.866 kb on - strand, within ABIE41_RS04820 at 1008.866 kb on - strand, within ABIE41_RS04820 at 1008.866 kb on - strand, within ABIE41_RS04820 at 1009.078 kb on + strand at 1009.078 kb on + strand at 1009.078 kb on + strand at 1009.078 kb on + strand at 1009.078 kb on + strand at 1009.078 kb on + strand at 1009.078 kb on + strand at 1009.079 kb on - strand at 1009.079 kb on - strand at 1009.079 kb on - strand at 1009.079 kb on - strand at 1009.079 kb on - strand at 1009.079 kb on - strand at 1009.269 kb on + strand, within ABIE41_RS04825 at 1009.269 kb on + strand, within ABIE41_RS04825 at 1009.269 kb on + strand, within ABIE41_RS04825 at 1009.270 kb on - strand, within ABIE41_RS04825 at 1009.270 kb on - strand, within ABIE41_RS04825 at 1009.353 kb on + strand, within ABIE41_RS04825 at 1009.416 kb on + strand, within ABIE41_RS04825 at 1009.416 kb on + strand, within ABIE41_RS04825 at 1009.416 kb on + strand, within ABIE41_RS04825 at 1009.416 kb on + strand, within ABIE41_RS04825 at 1009.417 kb on - strand, within ABIE41_RS04825 at 1009.664 kb on + strand, within ABIE41_RS04825 at 1009.664 kb on + strand, within ABIE41_RS04825 at 1009.665 kb on + strand, within ABIE41_RS04825 at 1009.665 kb on + strand, within ABIE41_RS04825 at 1009.665 kb on + strand, within ABIE41_RS04825 at 1009.665 kb on + strand, within ABIE41_RS04825 at 1009.665 kb on + strand, within ABIE41_RS04825 at 1009.665 kb on + strand, within ABIE41_RS04825 at 1009.665 kb on + strand, within ABIE41_RS04825 at 1009.666 kb on - strand, within ABIE41_RS04825 at 1009.666 kb on - strand, within ABIE41_RS04825 at 1009.666 kb on - strand, within ABIE41_RS04825 at 1009.666 kb on - strand, within ABIE41_RS04825 at 1009.666 kb on - strand, within ABIE41_RS04825 at 1009.683 kb on + strand, within ABIE41_RS04825 at 1009.684 kb on - strand, within ABIE41_RS04825 at 1009.702 kb on - strand at 1009.840 kb on + strand, within ABIE41_RS04830 at 1009.841 kb on + strand, within ABIE41_RS04830 at 1009.841 kb on + strand, within ABIE41_RS04830 at 1009.842 kb on - strand, within ABIE41_RS04830 at 1009.842 kb on - strand, within ABIE41_RS04830 at 1009.842 kb on - strand, within ABIE41_RS04830 at 1009.937 kb on + strand, within ABIE41_RS04830 at 1009.938 kb on - strand, within ABIE41_RS04830 at 1010.033 kb on + strand at 1010.034 kb on - strand at 1010.046 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.542729333333333 mM remove 1,007,085 + -0.2 1,007,086 - +0.2 1,007,086 - +0.4 1,007,086 - -0.1 1,007,086 - +0.5 1,007,086 - -0.4 1,007,414 - ABIE41_RS04815 0.14 +1.0 1,007,414 - ABIE41_RS04815 0.14 +0.0 1,007,652 + ABIE41_RS04815 0.54 +0.9 1,007,652 + ABIE41_RS04815 0.54 -1.2 1,007,652 + ABIE41_RS04815 0.54 -0.4 1,007,653 - ABIE41_RS04815 0.54 +1.1 1,007,653 - ABIE41_RS04815 0.54 -0.2 1,007,653 - ABIE41_RS04815 0.54 -0.4 1,007,653 - ABIE41_RS04815 0.54 -1.0 1,007,653 - ABIE41_RS04815 0.54 +0.0 1,007,698 + ABIE41_RS04815 0.61 -0.4 1,007,698 + ABIE41_RS04815 0.61 +1.5 1,007,698 + ABIE41_RS04815 0.61 +0.4 1,007,698 + ABIE41_RS04815 0.61 -2.0 1,007,698 + ABIE41_RS04815 0.61 -0.4 1,007,699 - ABIE41_RS04815 0.61 -0.1 1,007,699 - ABIE41_RS04815 0.61 -0.2 1,007,699 - ABIE41_RS04815 0.61 -0.1 1,007,699 - ABIE41_RS04815 0.61 +0.4 1,007,699 - ABIE41_RS04815 0.61 +1.0 1,007,699 - ABIE41_RS04815 0.61 +1.5 1,007,725 + ABIE41_RS04815 0.66 +0.3 1,007,725 + ABIE41_RS04815 0.66 +0.7 1,007,725 + ABIE41_RS04815 0.66 +0.8 1,007,725 + ABIE41_RS04815 0.66 +0.1 1,007,725 + ABIE41_RS04815 0.66 -1.5 1,007,725 + ABIE41_RS04815 0.66 -0.6 1,007,726 - ABIE41_RS04815 0.66 -0.1 1,007,726 - ABIE41_RS04815 0.66 +1.2 1,007,726 - ABIE41_RS04815 0.66 -0.1 1,007,726 - ABIE41_RS04815 0.66 -1.8 1,007,726 - ABIE41_RS04815 0.66 -0.5 1,007,726 - ABIE41_RS04815 0.66 +0.9 1,007,726 - ABIE41_RS04815 0.66 -0.3 1,007,726 - ABIE41_RS04815 0.66 -0.3 1,007,726 - ABIE41_RS04815 0.66 +1.0 1,007,749 + ABIE41_RS04815 0.69 +1.2 1,007,750 - ABIE41_RS04815 0.70 +2.6 1,007,750 - ABIE41_RS04815 0.70 +0.1 1,007,750 - ABIE41_RS04815 0.70 +1.1 1,007,750 - ABIE41_RS04815 0.70 +0.2 1,007,750 - ABIE41_RS04815 0.70 +0.7 1,007,786 - ABIE41_RS04815 0.76 -0.1 1,007,786 - ABIE41_RS04815 0.76 +0.8 1,007,893 - +0.0 1,007,893 - +0.9 1,008,017 + +0.7 1,008,017 + -0.4 1,008,018 - -0.1 1,008,026 - +0.1 1,008,028 + -1.4 1,008,028 + -0.6 1,008,029 - +0.6 1,008,088 + -0.4 1,008,088 + +0.8 1,008,088 + -0.1 1,008,089 - -0.5 1,008,089 - -0.7 1,008,089 - +2.9 1,008,134 - ABIE41_RS04820 0.11 -0.3 1,008,134 - ABIE41_RS04820 0.11 -0.3 1,008,134 - ABIE41_RS04820 0.11 -2.1 1,008,134 - ABIE41_RS04820 0.11 +1.5 1,008,134 - ABIE41_RS04820 0.11 -0.3 1,008,178 + ABIE41_RS04820 0.15 -1.8 1,008,178 + ABIE41_RS04820 0.15 +0.1 1,008,178 + ABIE41_RS04820 0.15 -1.6 1,008,179 - ABIE41_RS04820 0.15 -0.0 1,008,397 + ABIE41_RS04820 0.36 +2.5 1,008,397 + ABIE41_RS04820 0.36 +2.1 1,008,397 + ABIE41_RS04820 0.36 -1.3 1,008,397 + ABIE41_RS04820 0.36 -0.7 1,008,397 + ABIE41_RS04820 0.36 -0.4 1,008,397 + ABIE41_RS04820 0.36 -0.8 1,008,397 + ABIE41_RS04820 0.36 +0.5 1,008,398 - ABIE41_RS04820 0.36 +0.7 1,008,398 - ABIE41_RS04820 0.36 +0.3 1,008,398 - ABIE41_RS04820 0.36 +0.0 1,008,398 - ABIE41_RS04820 0.36 -0.4 1,008,399 + ABIE41_RS04820 0.36 -0.4 1,008,399 + ABIE41_RS04820 0.36 +0.3 1,008,400 - ABIE41_RS04820 0.36 -0.2 1,008,400 - ABIE41_RS04820 0.36 +1.3 1,008,400 - ABIE41_RS04820 0.36 -0.5 1,008,400 - ABIE41_RS04820 0.36 -1.3 1,008,400 - ABIE41_RS04820 0.36 -0.7 1,008,400 - ABIE41_RS04820 0.36 -0.8 1,008,410 - ABIE41_RS04820 0.37 -0.6 1,008,410 - ABIE41_RS04820 0.37 +0.3 1,008,423 - ABIE41_RS04820 0.38 -1.9 1,008,659 - ABIE41_RS04820 0.60 +0.8 1,008,733 + ABIE41_RS04820 0.67 -0.3 1,008,734 - ABIE41_RS04820 0.67 +0.3 1,008,769 + ABIE41_RS04820 0.71 +0.9 1,008,769 + ABIE41_RS04820 0.71 +0.1 1,008,769 + ABIE41_RS04820 0.71 -1.6 1,008,770 - ABIE41_RS04820 0.71 -1.2 1,008,770 - ABIE41_RS04820 0.71 -0.1 1,008,864 + ABIE41_RS04820 0.80 +0.1 1,008,865 + ABIE41_RS04820 0.80 -0.5 1,008,865 + ABIE41_RS04820 0.80 -0.6 1,008,865 + ABIE41_RS04820 0.80 +0.7 1,008,865 + ABIE41_RS04820 0.80 -1.4 1,008,865 + ABIE41_RS04820 0.80 +0.8 1,008,866 - ABIE41_RS04820 0.80 -0.4 1,008,866 - ABIE41_RS04820 0.80 +0.3 1,008,866 - ABIE41_RS04820 0.80 -0.0 1,008,866 - ABIE41_RS04820 0.80 -0.4 1,009,078 + +0.4 1,009,078 + -1.6 1,009,078 + -0.1 1,009,078 + -0.1 1,009,078 + -1.8 1,009,078 + -0.2 1,009,078 + -0.4 1,009,079 - +0.2 1,009,079 - +1.4 1,009,079 - +0.2 1,009,079 - +0.0 1,009,079 - +0.5 1,009,079 - -0.7 1,009,269 + ABIE41_RS04825 0.18 -0.7 1,009,269 + ABIE41_RS04825 0.18 -0.4 1,009,269 + ABIE41_RS04825 0.18 -0.9 1,009,270 - ABIE41_RS04825 0.18 +1.0 1,009,270 - ABIE41_RS04825 0.18 +0.3 1,009,353 + ABIE41_RS04825 0.33 -1.1 1,009,416 + ABIE41_RS04825 0.44 +1.0 1,009,416 + ABIE41_RS04825 0.44 +0.6 1,009,416 + ABIE41_RS04825 0.44 +1.8 1,009,416 + ABIE41_RS04825 0.44 +2.2 1,009,417 - ABIE41_RS04825 0.44 +1.6 1,009,664 + ABIE41_RS04825 0.86 +1.3 1,009,664 + ABIE41_RS04825 0.86 -0.2 1,009,665 + ABIE41_RS04825 0.87 -0.5 1,009,665 + ABIE41_RS04825 0.87 -0.0 1,009,665 + ABIE41_RS04825 0.87 -1.4 1,009,665 + ABIE41_RS04825 0.87 +0.1 1,009,665 + ABIE41_RS04825 0.87 -1.1 1,009,665 + ABIE41_RS04825 0.87 -0.5 1,009,665 + ABIE41_RS04825 0.87 +0.3 1,009,666 - ABIE41_RS04825 0.87 -0.5 1,009,666 - ABIE41_RS04825 0.87 +1.4 1,009,666 - ABIE41_RS04825 0.87 +0.8 1,009,666 - ABIE41_RS04825 0.87 +0.0 1,009,666 - ABIE41_RS04825 0.87 -0.4 1,009,683 + ABIE41_RS04825 0.90 +0.5 1,009,684 - ABIE41_RS04825 0.90 +1.0 1,009,702 - +0.6 1,009,840 + ABIE41_RS04830 0.33 +0.8 1,009,841 + ABIE41_RS04830 0.33 -2.1 1,009,841 + ABIE41_RS04830 0.33 -0.9 1,009,842 - ABIE41_RS04830 0.33 +1.0 1,009,842 - ABIE41_RS04830 0.33 +0.8 1,009,842 - ABIE41_RS04830 0.33 -2.4 1,009,937 + ABIE41_RS04830 0.66 +0.7 1,009,938 - ABIE41_RS04830 0.66 -2.6 1,010,033 + -0.2 1,010,034 - -0.3 1,010,046 + +0.9
Or see this region's nucleotide sequence