Experiment: R2A with 1-octen-3-ol 0.603032666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS10580 and ABIE41_RS10585 are separated by 32 nucleotides ABIE41_RS10585 and ABIE41_RS10590 overlap by 4 nucleotides ABIE41_RS10590 and ABIE41_RS10595 overlap by 4 nucleotides
ABIE41_RS10580: ABIE41_RS10580 - creatininase family protein, at 2,183,214 to 2,184,041
_RS10580
ABIE41_RS10585: ABIE41_RS10585 - ABC transporter permease, at 2,184,074 to 2,184,895
_RS10585
ABIE41_RS10590: ABIE41_RS10590 - ABC transporter permease, at 2,184,892 to 2,185,848
_RS10590
ABIE41_RS10595: ABIE41_RS10595 - ABC transporter substrate-binding protein, at 2,185,845 to 2,187,377
_RS10595
Position (kb)
2184
2185
2186 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2184.011 kb on + strand at 2184.366 kb on + strand, within ABIE41_RS10585 at 2184.465 kb on + strand, within ABIE41_RS10585 at 2184.465 kb on + strand, within ABIE41_RS10585 at 2184.465 kb on + strand, within ABIE41_RS10585 at 2184.465 kb on + strand, within ABIE41_RS10585 at 2184.466 kb on - strand, within ABIE41_RS10585 at 2184.466 kb on - strand, within ABIE41_RS10585 at 2184.466 kb on - strand, within ABIE41_RS10585 at 2185.061 kb on + strand, within ABIE41_RS10590 at 2185.061 kb on + strand, within ABIE41_RS10590 at 2185.062 kb on - strand, within ABIE41_RS10590 at 2185.202 kb on + strand, within ABIE41_RS10590 at 2185.202 kb on + strand, within ABIE41_RS10590 at 2185.202 kb on + strand, within ABIE41_RS10590 at 2185.202 kb on + strand, within ABIE41_RS10590 at 2185.202 kb on + strand, within ABIE41_RS10590 at 2185.202 kb on + strand, within ABIE41_RS10590 at 2185.203 kb on - strand, within ABIE41_RS10590 at 2185.203 kb on - strand, within ABIE41_RS10590 at 2185.203 kb on - strand, within ABIE41_RS10590 at 2185.203 kb on - strand, within ABIE41_RS10590 at 2185.203 kb on - strand, within ABIE41_RS10590 at 2185.203 kb on - strand, within ABIE41_RS10590 at 2185.644 kb on - strand, within ABIE41_RS10590 at 2185.644 kb on - strand, within ABIE41_RS10590 at 2185.644 kb on - strand, within ABIE41_RS10590 at 2185.855 kb on + strand at 2185.855 kb on + strand at 2185.856 kb on - strand at 2185.856 kb on - strand at 2185.856 kb on - strand at 2185.940 kb on - strand at 2186.008 kb on + strand, within ABIE41_RS10595 at 2186.008 kb on + strand, within ABIE41_RS10595 at 2186.008 kb on + strand, within ABIE41_RS10595 at 2186.008 kb on + strand, within ABIE41_RS10595 at 2186.008 kb on + strand, within ABIE41_RS10595 at 2186.188 kb on + strand, within ABIE41_RS10595 at 2186.188 kb on + strand, within ABIE41_RS10595 at 2186.188 kb on + strand, within ABIE41_RS10595 at 2186.392 kb on + strand, within ABIE41_RS10595 at 2186.392 kb on + strand, within ABIE41_RS10595 at 2186.393 kb on - strand, within ABIE41_RS10595 at 2186.393 kb on - strand, within ABIE41_RS10595 at 2186.393 kb on - strand, within ABIE41_RS10595 at 2186.393 kb on - strand, within ABIE41_RS10595 at 2186.656 kb on + strand, within ABIE41_RS10595 at 2186.656 kb on + strand, within ABIE41_RS10595 at 2186.657 kb on - strand, within ABIE41_RS10595 at 2186.657 kb on - strand, within ABIE41_RS10595 at 2186.657 kb on - strand, within ABIE41_RS10595 at 2186.657 kb on - strand, within ABIE41_RS10595 at 2186.661 kb on + strand, within ABIE41_RS10595 at 2186.661 kb on + strand, within ABIE41_RS10595 at 2186.661 kb on + strand, within ABIE41_RS10595 at 2186.661 kb on + strand, within ABIE41_RS10595 at 2186.661 kb on + strand, within ABIE41_RS10595 at 2186.661 kb on + strand, within ABIE41_RS10595 at 2186.661 kb on + strand, within ABIE41_RS10595 at 2186.661 kb on + strand, within ABIE41_RS10595 at 2186.661 kb on + strand, within ABIE41_RS10595 at 2186.662 kb on - strand, within ABIE41_RS10595 at 2186.662 kb on - strand, within ABIE41_RS10595 at 2186.662 kb on - strand, within ABIE41_RS10595 at 2186.662 kb on - strand, within ABIE41_RS10595 at 2186.716 kb on + strand, within ABIE41_RS10595 at 2186.716 kb on + strand, within ABIE41_RS10595 at 2186.716 kb on + strand, within ABIE41_RS10595 at 2186.716 kb on + strand, within ABIE41_RS10595 at 2186.761 kb on + strand, within ABIE41_RS10595 at 2186.762 kb on - strand, within ABIE41_RS10595 at 2186.762 kb on - strand, within ABIE41_RS10595
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.603032666666667 mM remove 2,184,011 + -0.5 2,184,366 + ABIE41_RS10585 0.36 -0.6 2,184,465 + ABIE41_RS10585 0.48 -0.7 2,184,465 + ABIE41_RS10585 0.48 -0.7 2,184,465 + ABIE41_RS10585 0.48 -1.9 2,184,465 + ABIE41_RS10585 0.48 +0.5 2,184,466 - ABIE41_RS10585 0.48 -2.2 2,184,466 - ABIE41_RS10585 0.48 -0.5 2,184,466 - ABIE41_RS10585 0.48 -0.2 2,185,061 + ABIE41_RS10590 0.18 -0.1 2,185,061 + ABIE41_RS10590 0.18 -1.6 2,185,062 - ABIE41_RS10590 0.18 -2.8 2,185,202 + ABIE41_RS10590 0.32 +0.4 2,185,202 + ABIE41_RS10590 0.32 -0.4 2,185,202 + ABIE41_RS10590 0.32 +1.6 2,185,202 + ABIE41_RS10590 0.32 -0.2 2,185,202 + ABIE41_RS10590 0.32 +3.6 2,185,202 + ABIE41_RS10590 0.32 -0.3 2,185,203 - ABIE41_RS10590 0.32 +0.7 2,185,203 - ABIE41_RS10590 0.32 +0.1 2,185,203 - ABIE41_RS10590 0.32 -0.0 2,185,203 - ABIE41_RS10590 0.32 +2.0 2,185,203 - ABIE41_RS10590 0.32 -0.6 2,185,203 - ABIE41_RS10590 0.32 -0.1 2,185,644 - ABIE41_RS10590 0.79 +0.4 2,185,644 - ABIE41_RS10590 0.79 -2.1 2,185,644 - ABIE41_RS10590 0.79 -0.5 2,185,855 + -0.0 2,185,855 + -0.2 2,185,856 - +0.9 2,185,856 - +0.4 2,185,856 - -0.3 2,185,940 - -0.5 2,186,008 + ABIE41_RS10595 0.11 +0.2 2,186,008 + ABIE41_RS10595 0.11 +0.1 2,186,008 + ABIE41_RS10595 0.11 +0.3 2,186,008 + ABIE41_RS10595 0.11 +1.4 2,186,008 + ABIE41_RS10595 0.11 +0.3 2,186,188 + ABIE41_RS10595 0.22 +0.0 2,186,188 + ABIE41_RS10595 0.22 +0.6 2,186,188 + ABIE41_RS10595 0.22 +0.4 2,186,392 + ABIE41_RS10595 0.36 +0.2 2,186,392 + ABIE41_RS10595 0.36 +2.9 2,186,393 - ABIE41_RS10595 0.36 +0.3 2,186,393 - ABIE41_RS10595 0.36 -0.7 2,186,393 - ABIE41_RS10595 0.36 -0.4 2,186,393 - ABIE41_RS10595 0.36 +0.2 2,186,656 + ABIE41_RS10595 0.53 -0.7 2,186,656 + ABIE41_RS10595 0.53 +0.6 2,186,657 - ABIE41_RS10595 0.53 +1.5 2,186,657 - ABIE41_RS10595 0.53 -0.9 2,186,657 - ABIE41_RS10595 0.53 -1.8 2,186,657 - ABIE41_RS10595 0.53 -0.6 2,186,661 + ABIE41_RS10595 0.53 -0.0 2,186,661 + ABIE41_RS10595 0.53 -0.0 2,186,661 + ABIE41_RS10595 0.53 -1.1 2,186,661 + ABIE41_RS10595 0.53 +0.7 2,186,661 + ABIE41_RS10595 0.53 +3.1 2,186,661 + ABIE41_RS10595 0.53 +0.3 2,186,661 + ABIE41_RS10595 0.53 +1.2 2,186,661 + ABIE41_RS10595 0.53 -0.3 2,186,661 + ABIE41_RS10595 0.53 -0.2 2,186,662 - ABIE41_RS10595 0.53 +0.2 2,186,662 - ABIE41_RS10595 0.53 +0.6 2,186,662 - ABIE41_RS10595 0.53 +1.2 2,186,662 - ABIE41_RS10595 0.53 +0.9 2,186,716 + ABIE41_RS10595 0.57 -2.6 2,186,716 + ABIE41_RS10595 0.57 +0.0 2,186,716 + ABIE41_RS10595 0.57 -1.8 2,186,716 + ABIE41_RS10595 0.57 +0.3 2,186,761 + ABIE41_RS10595 0.60 +0.6 2,186,762 - ABIE41_RS10595 0.60 +1.0 2,186,762 - ABIE41_RS10595 0.60 +0.8
Or see this region's nucleotide sequence