Experiment: R2A with 1-octen-3-ol 0.603032666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS05250 and ABIE41_RS05255 are separated by 2 nucleotides ABIE41_RS05255 and ABIE41_RS05260 are separated by 169 nucleotides
ABIE41_RS05250: ABIE41_RS05250 - carbohydrate ABC transporter permease, at 1,086,328 to 1,087,380
_RS05250
ABIE41_RS05255: ABIE41_RS05255 - sn-glycerol-3-phosphate ABC transporter ATP-binding protein UgpC, at 1,087,383 to 1,088,447
_RS05255
ABIE41_RS05260: ABIE41_RS05260 - TIM-barrel domain-containing protein, at 1,088,617 to 1,090,905
_RS05260
Position (kb)
1087
1088
1089 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1086.400 kb on + strand at 1086.400 kb on + strand at 1086.401 kb on - strand at 1086.401 kb on - strand at 1086.406 kb on + strand at 1086.407 kb on - strand at 1086.727 kb on + strand, within ABIE41_RS05250 at 1086.727 kb on + strand, within ABIE41_RS05250 at 1086.728 kb on - strand, within ABIE41_RS05250 at 1086.835 kb on + strand, within ABIE41_RS05250 at 1086.836 kb on - strand, within ABIE41_RS05250 at 1086.836 kb on - strand, within ABIE41_RS05250 at 1086.836 kb on - strand, within ABIE41_RS05250 at 1086.836 kb on - strand, within ABIE41_RS05250 at 1086.836 kb on - strand, within ABIE41_RS05250 at 1086.934 kb on + strand, within ABIE41_RS05250 at 1087.031 kb on - strand, within ABIE41_RS05250 at 1087.031 kb on - strand, within ABIE41_RS05250 at 1087.031 kb on - strand, within ABIE41_RS05250 at 1087.031 kb on - strand, within ABIE41_RS05250 at 1087.099 kb on + strand, within ABIE41_RS05250 at 1087.099 kb on + strand, within ABIE41_RS05250 at 1087.099 kb on + strand, within ABIE41_RS05250 at 1087.099 kb on + strand, within ABIE41_RS05250 at 1087.100 kb on - strand, within ABIE41_RS05250 at 1087.100 kb on - strand, within ABIE41_RS05250 at 1087.219 kb on + strand, within ABIE41_RS05250 at 1087.219 kb on + strand, within ABIE41_RS05250 at 1087.219 kb on + strand, within ABIE41_RS05250 at 1087.220 kb on - strand, within ABIE41_RS05250 at 1087.276 kb on + strand at 1087.277 kb on - strand at 1087.277 kb on - strand at 1087.378 kb on + strand at 1087.378 kb on + strand at 1087.379 kb on - strand at 1087.379 kb on - strand at 1087.379 kb on - strand at 1087.379 kb on - strand at 1087.379 kb on - strand at 1087.381 kb on - strand at 1087.632 kb on + strand, within ABIE41_RS05255 at 1087.633 kb on - strand, within ABIE41_RS05255 at 1087.649 kb on + strand, within ABIE41_RS05255 at 1087.650 kb on - strand, within ABIE41_RS05255 at 1087.650 kb on - strand, within ABIE41_RS05255 at 1087.650 kb on - strand, within ABIE41_RS05255 at 1087.650 kb on - strand, within ABIE41_RS05255 at 1087.659 kb on + strand, within ABIE41_RS05255 at 1087.659 kb on + strand, within ABIE41_RS05255 at 1087.660 kb on - strand, within ABIE41_RS05255 at 1087.660 kb on - strand, within ABIE41_RS05255 at 1087.688 kb on + strand, within ABIE41_RS05255 at 1087.689 kb on - strand, within ABIE41_RS05255 at 1087.689 kb on - strand, within ABIE41_RS05255 at 1087.689 kb on - strand, within ABIE41_RS05255 at 1087.759 kb on - strand, within ABIE41_RS05255 at 1087.947 kb on + strand, within ABIE41_RS05255 at 1087.947 kb on + strand, within ABIE41_RS05255 at 1087.947 kb on + strand, within ABIE41_RS05255 at 1087.947 kb on + strand, within ABIE41_RS05255 at 1087.948 kb on - strand, within ABIE41_RS05255 at 1087.948 kb on - strand, within ABIE41_RS05255 at 1087.948 kb on - strand, within ABIE41_RS05255 at 1087.948 kb on - strand, within ABIE41_RS05255 at 1087.948 kb on - strand, within ABIE41_RS05255 at 1087.948 kb on - strand, within ABIE41_RS05255 at 1087.948 kb on - strand, within ABIE41_RS05255 at 1087.948 kb on - strand, within ABIE41_RS05255 at 1087.948 kb on - strand, within ABIE41_RS05255 at 1088.046 kb on + strand, within ABIE41_RS05255 at 1088.046 kb on + strand, within ABIE41_RS05255 at 1088.047 kb on - strand, within ABIE41_RS05255 at 1088.047 kb on - strand, within ABIE41_RS05255 at 1088.047 kb on - strand, within ABIE41_RS05255 at 1088.047 kb on - strand, within ABIE41_RS05255 at 1088.298 kb on + strand, within ABIE41_RS05255 at 1088.298 kb on + strand, within ABIE41_RS05255 at 1088.299 kb on - strand, within ABIE41_RS05255 at 1088.299 kb on - strand, within ABIE41_RS05255 at 1088.299 kb on - strand, within ABIE41_RS05255 at 1088.594 kb on - strand at 1088.641 kb on + strand at 1088.642 kb on - strand at 1088.806 kb on + strand at 1088.807 kb on - strand at 1088.807 kb on - strand at 1088.995 kb on + strand, within ABIE41_RS05260 at 1088.995 kb on + strand, within ABIE41_RS05260 at 1088.995 kb on + strand, within ABIE41_RS05260 at 1088.995 kb on + strand, within ABIE41_RS05260 at 1088.995 kb on + strand, within ABIE41_RS05260 at 1088.995 kb on + strand, within ABIE41_RS05260 at 1088.995 kb on + strand, within ABIE41_RS05260 at 1089.031 kb on + strand, within ABIE41_RS05260 at 1089.031 kb on + strand, within ABIE41_RS05260 at 1089.032 kb on - strand, within ABIE41_RS05260 at 1089.032 kb on - strand, within ABIE41_RS05260 at 1089.032 kb on - strand, within ABIE41_RS05260 at 1089.032 kb on - strand, within ABIE41_RS05260 at 1089.032 kb on - strand, within ABIE41_RS05260 at 1089.032 kb on - strand, within ABIE41_RS05260 at 1089.032 kb on - strand, within ABIE41_RS05260 at 1089.047 kb on - strand, within ABIE41_RS05260 at 1089.166 kb on + strand, within ABIE41_RS05260 at 1089.166 kb on + strand, within ABIE41_RS05260 at 1089.166 kb on + strand, within ABIE41_RS05260 at 1089.166 kb on + strand, within ABIE41_RS05260 at 1089.166 kb on + strand, within ABIE41_RS05260 at 1089.224 kb on - strand, within ABIE41_RS05260 at 1089.224 kb on - strand, within ABIE41_RS05260 at 1089.224 kb on - strand, within ABIE41_RS05260 at 1089.224 kb on - strand, within ABIE41_RS05260 at 1089.274 kb on + strand, within ABIE41_RS05260 at 1089.274 kb on + strand, within ABIE41_RS05260 at 1089.274 kb on + strand, within ABIE41_RS05260 at 1089.274 kb on + strand, within ABIE41_RS05260 at 1089.274 kb on + strand, within ABIE41_RS05260 at 1089.275 kb on - strand, within ABIE41_RS05260 at 1089.275 kb on - strand, within ABIE41_RS05260 at 1089.325 kb on + strand, within ABIE41_RS05260 at 1089.325 kb on + strand, within ABIE41_RS05260 at 1089.325 kb on + strand, within ABIE41_RS05260 at 1089.325 kb on + strand, within ABIE41_RS05260 at 1089.326 kb on - strand, within ABIE41_RS05260 at 1089.326 kb on - strand, within ABIE41_RS05260 at 1089.326 kb on - strand, within ABIE41_RS05260 at 1089.326 kb on - strand, within ABIE41_RS05260 at 1089.412 kb on + strand, within ABIE41_RS05260 at 1089.412 kb on + strand, within ABIE41_RS05260 at 1089.413 kb on - strand, within ABIE41_RS05260
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.603032666666667 mM remove 1,086,400 + +0.1 1,086,400 + +0.1 1,086,401 - +0.4 1,086,401 - -0.3 1,086,406 + -0.5 1,086,407 - -0.2 1,086,727 + ABIE41_RS05250 0.38 +0.4 1,086,727 + ABIE41_RS05250 0.38 -0.1 1,086,728 - ABIE41_RS05250 0.38 +0.4 1,086,835 + ABIE41_RS05250 0.48 -0.3 1,086,836 - ABIE41_RS05250 0.48 +0.7 1,086,836 - ABIE41_RS05250 0.48 +0.3 1,086,836 - ABIE41_RS05250 0.48 +0.9 1,086,836 - ABIE41_RS05250 0.48 -0.2 1,086,836 - ABIE41_RS05250 0.48 -0.0 1,086,934 + ABIE41_RS05250 0.58 +0.1 1,087,031 - ABIE41_RS05250 0.67 +1.3 1,087,031 - ABIE41_RS05250 0.67 +2.2 1,087,031 - ABIE41_RS05250 0.67 +0.0 1,087,031 - ABIE41_RS05250 0.67 -0.2 1,087,099 + ABIE41_RS05250 0.73 -0.7 1,087,099 + ABIE41_RS05250 0.73 -0.6 1,087,099 + ABIE41_RS05250 0.73 +0.9 1,087,099 + ABIE41_RS05250 0.73 +0.7 1,087,100 - ABIE41_RS05250 0.73 +0.5 1,087,100 - ABIE41_RS05250 0.73 -0.0 1,087,219 + ABIE41_RS05250 0.85 +0.4 1,087,219 + ABIE41_RS05250 0.85 +1.6 1,087,219 + ABIE41_RS05250 0.85 -0.3 1,087,220 - ABIE41_RS05250 0.85 -0.1 1,087,276 + -0.9 1,087,277 - +1.3 1,087,277 - +0.1 1,087,378 + -0.1 1,087,378 + +0.1 1,087,379 - +3.9 1,087,379 - -0.5 1,087,379 - +0.6 1,087,379 - +0.5 1,087,379 - +1.7 1,087,381 - -1.3 1,087,632 + ABIE41_RS05255 0.23 -0.5 1,087,633 - ABIE41_RS05255 0.23 +0.3 1,087,649 + ABIE41_RS05255 0.25 -1.0 1,087,650 - ABIE41_RS05255 0.25 -0.3 1,087,650 - ABIE41_RS05255 0.25 +0.1 1,087,650 - ABIE41_RS05255 0.25 -0.3 1,087,650 - ABIE41_RS05255 0.25 -1.0 1,087,659 + ABIE41_RS05255 0.26 -1.2 1,087,659 + ABIE41_RS05255 0.26 -1.1 1,087,660 - ABIE41_RS05255 0.26 -0.5 1,087,660 - ABIE41_RS05255 0.26 -0.2 1,087,688 + ABIE41_RS05255 0.29 +0.6 1,087,689 - ABIE41_RS05255 0.29 +0.7 1,087,689 - ABIE41_RS05255 0.29 +0.7 1,087,689 - ABIE41_RS05255 0.29 +0.2 1,087,759 - ABIE41_RS05255 0.35 +0.3 1,087,947 + ABIE41_RS05255 0.53 +0.1 1,087,947 + ABIE41_RS05255 0.53 -1.6 1,087,947 + ABIE41_RS05255 0.53 +0.9 1,087,947 + ABIE41_RS05255 0.53 -0.4 1,087,948 - ABIE41_RS05255 0.53 -0.0 1,087,948 - ABIE41_RS05255 0.53 -0.2 1,087,948 - ABIE41_RS05255 0.53 -0.2 1,087,948 - ABIE41_RS05255 0.53 +0.3 1,087,948 - ABIE41_RS05255 0.53 +1.5 1,087,948 - ABIE41_RS05255 0.53 -0.4 1,087,948 - ABIE41_RS05255 0.53 +0.1 1,087,948 - ABIE41_RS05255 0.53 -0.4 1,087,948 - ABIE41_RS05255 0.53 -1.0 1,088,046 + ABIE41_RS05255 0.62 +0.1 1,088,046 + ABIE41_RS05255 0.62 -1.9 1,088,047 - ABIE41_RS05255 0.62 +0.0 1,088,047 - ABIE41_RS05255 0.62 -0.9 1,088,047 - ABIE41_RS05255 0.62 -0.7 1,088,047 - ABIE41_RS05255 0.62 -0.0 1,088,298 + ABIE41_RS05255 0.86 +0.6 1,088,298 + ABIE41_RS05255 0.86 +0.2 1,088,299 - ABIE41_RS05255 0.86 -0.6 1,088,299 - ABIE41_RS05255 0.86 -0.4 1,088,299 - ABIE41_RS05255 0.86 -2.1 1,088,594 - -0.4 1,088,641 + +0.4 1,088,642 - +1.9 1,088,806 + +0.2 1,088,807 - +0.2 1,088,807 - +0.2 1,088,995 + ABIE41_RS05260 0.17 +1.0 1,088,995 + ABIE41_RS05260 0.17 -0.9 1,088,995 + ABIE41_RS05260 0.17 +1.2 1,088,995 + ABIE41_RS05260 0.17 +0.4 1,088,995 + ABIE41_RS05260 0.17 -2.0 1,088,995 + ABIE41_RS05260 0.17 -0.4 1,088,995 + ABIE41_RS05260 0.17 -0.3 1,089,031 + ABIE41_RS05260 0.18 -0.6 1,089,031 + ABIE41_RS05260 0.18 -0.4 1,089,032 - ABIE41_RS05260 0.18 -1.7 1,089,032 - ABIE41_RS05260 0.18 +0.5 1,089,032 - ABIE41_RS05260 0.18 -0.0 1,089,032 - ABIE41_RS05260 0.18 -0.2 1,089,032 - ABIE41_RS05260 0.18 +0.4 1,089,032 - ABIE41_RS05260 0.18 +1.0 1,089,032 - ABIE41_RS05260 0.18 +1.0 1,089,047 - ABIE41_RS05260 0.19 -0.2 1,089,166 + ABIE41_RS05260 0.24 +1.5 1,089,166 + ABIE41_RS05260 0.24 +0.0 1,089,166 + ABIE41_RS05260 0.24 -0.3 1,089,166 + ABIE41_RS05260 0.24 -0.2 1,089,166 + ABIE41_RS05260 0.24 -0.0 1,089,224 - ABIE41_RS05260 0.27 +0.4 1,089,224 - ABIE41_RS05260 0.27 +0.9 1,089,224 - ABIE41_RS05260 0.27 +0.3 1,089,224 - ABIE41_RS05260 0.27 -1.3 1,089,274 + ABIE41_RS05260 0.29 +0.1 1,089,274 + ABIE41_RS05260 0.29 +1.2 1,089,274 + ABIE41_RS05260 0.29 +0.3 1,089,274 + ABIE41_RS05260 0.29 +1.9 1,089,274 + ABIE41_RS05260 0.29 -1.7 1,089,275 - ABIE41_RS05260 0.29 +1.0 1,089,275 - ABIE41_RS05260 0.29 +0.6 1,089,325 + ABIE41_RS05260 0.31 +0.2 1,089,325 + ABIE41_RS05260 0.31 -0.1 1,089,325 + ABIE41_RS05260 0.31 +0.4 1,089,325 + ABIE41_RS05260 0.31 +0.1 1,089,326 - ABIE41_RS05260 0.31 +0.2 1,089,326 - ABIE41_RS05260 0.31 +0.3 1,089,326 - ABIE41_RS05260 0.31 -1.6 1,089,326 - ABIE41_RS05260 0.31 +0.7 1,089,412 + ABIE41_RS05260 0.35 +0.9 1,089,412 + ABIE41_RS05260 0.35 -0.4 1,089,413 - ABIE41_RS05260 0.35 +0.0
Or see this region's nucleotide sequence