Experiment: R2A with 1-octen-3-ol 0.603032666666667 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS20200 and ABIE41_RS20205 are separated by 218 nucleotides ABIE41_RS20205 and ABIE41_RS20210 are separated by 212 nucleotides ABIE41_RS20210 and ABIE41_RS20215 are separated by 171 nucleotides
ABIE41_RS20200: ABIE41_RS20200 - mandelate racemase/muconate lactonizing enzyme family protein, at 4,153,013 to 4,154,131
_RS20200
ABIE41_RS20205: ABIE41_RS20205 - tRNA-Phe, at 4,154,350 to 4,154,425
_RS20205
ABIE41_RS20210: ABIE41_RS20210 - tRNA-Ser, at 4,154,638 to 4,154,728
_RS20210
ABIE41_RS20215: ABIE41_RS20215 - GntR family transcriptional regulator, at 4,154,900 to 4,155,568
_RS20215
Position (kb) 4154
4155 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 4153.708 kb on + strand, within ABIE41_RS20200 at 4153.708 kb on + strand, within ABIE41_RS20200 at 4153.708 kb on + strand, within ABIE41_RS20200 at 4153.709 kb on - strand, within ABIE41_RS20200 at 4153.710 kb on + strand, within ABIE41_RS20200 at 4153.710 kb on + strand, within ABIE41_RS20200 at 4153.711 kb on - strand, within ABIE41_RS20200 at 4153.711 kb on - strand, within ABIE41_RS20200 at 4153.711 kb on - strand, within ABIE41_RS20200 at 4153.926 kb on + strand, within ABIE41_RS20200 at 4153.927 kb on - strand, within ABIE41_RS20200 at 4153.927 kb on - strand, within ABIE41_RS20200 at 4154.095 kb on + strand at 4154.095 kb on + strand at 4154.096 kb on - strand at 4155.004 kb on + strand, within ABIE41_RS20215 at 4155.004 kb on + strand, within ABIE41_RS20215 at 4155.005 kb on - strand, within ABIE41_RS20215 at 4155.072 kb on + strand, within ABIE41_RS20215 at 4155.073 kb on - strand, within ABIE41_RS20215 at 4155.073 kb on - strand, within ABIE41_RS20215 at 4155.104 kb on + strand, within ABIE41_RS20215 at 4155.105 kb on - strand, within ABIE41_RS20215 at 4155.197 kb on - strand, within ABIE41_RS20215 at 4155.287 kb on + strand, within ABIE41_RS20215 at 4155.447 kb on + strand, within ABIE41_RS20215 at 4155.447 kb on + strand, within ABIE41_RS20215 at 4155.448 kb on - strand, within ABIE41_RS20215 at 4155.529 kb on - strand at 4155.529 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 1-octen-3-ol 0.603032666666667 mM remove 4,153,708 + ABIE41_RS20200 0.62 -0.2 4,153,708 + ABIE41_RS20200 0.62 +0.8 4,153,708 + ABIE41_RS20200 0.62 +0.4 4,153,709 - ABIE41_RS20200 0.62 -2.9 4,153,710 + ABIE41_RS20200 0.62 +0.2 4,153,710 + ABIE41_RS20200 0.62 +4.1 4,153,711 - ABIE41_RS20200 0.62 -1.1 4,153,711 - ABIE41_RS20200 0.62 -0.4 4,153,711 - ABIE41_RS20200 0.62 +0.2 4,153,926 + ABIE41_RS20200 0.82 -0.9 4,153,927 - ABIE41_RS20200 0.82 -0.6 4,153,927 - ABIE41_RS20200 0.82 +2.8 4,154,095 + -0.4 4,154,095 + -1.4 4,154,096 - +0.3 4,155,004 + ABIE41_RS20215 0.16 +1.5 4,155,004 + ABIE41_RS20215 0.16 +0.2 4,155,005 - ABIE41_RS20215 0.16 -0.4 4,155,072 + ABIE41_RS20215 0.26 -1.9 4,155,073 - ABIE41_RS20215 0.26 +0.3 4,155,073 - ABIE41_RS20215 0.26 +0.0 4,155,104 + ABIE41_RS20215 0.30 -0.5 4,155,105 - ABIE41_RS20215 0.31 -0.8 4,155,197 - ABIE41_RS20215 0.44 -0.4 4,155,287 + ABIE41_RS20215 0.58 -0.9 4,155,447 + ABIE41_RS20215 0.82 +0.6 4,155,447 + ABIE41_RS20215 0.82 -0.2 4,155,448 - ABIE41_RS20215 0.82 +0.3 4,155,529 - +1.2 4,155,529 - +0.6
Or see this region's nucleotide sequence