Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.2709965 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS20830 and ABIE41_RS20835 are separated by 63 nucleotides ABIE41_RS20835 and ABIE41_RS20840 are separated by 6 nucleotides ABIE41_RS20840 and ABIE41_RS20845 are separated by 91 nucleotides
ABIE41_RS20830: ABIE41_RS20830 - phosphatidylserine decarboxylase, at 4,282,103 to 4,282,915
_RS20830
ABIE41_RS20835: ABIE41_RS20835 - cytochrome P450, at 4,282,979 to 4,284,343
_RS20835
ABIE41_RS20840: ABIE41_RS20840 - hypothetical protein, at 4,284,350 to 4,284,838
_RS20840
ABIE41_RS20845: ABIE41_RS20845 - ABC transporter ATP-binding protein/permease, at 4,284,930 to 4,286,843
_RS20845
Position (kb) 4282
4283
4284
4285 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4282.963 kb on + strand at 4283.103 kb on - strand at 4283.103 kb on - strand at 4283.150 kb on + strand, within ABIE41_RS20835 at 4283.150 kb on + strand, within ABIE41_RS20835 at 4283.150 kb on + strand, within ABIE41_RS20835 at 4283.151 kb on - strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.534 kb on + strand, within ABIE41_RS20835 at 4283.535 kb on - strand, within ABIE41_RS20835 at 4283.546 kb on + strand, within ABIE41_RS20835 at 4283.546 kb on + strand, within ABIE41_RS20835 at 4283.547 kb on - strand, within ABIE41_RS20835 at 4283.547 kb on - strand, within ABIE41_RS20835 at 4283.615 kb on + strand, within ABIE41_RS20835 at 4283.616 kb on - strand, within ABIE41_RS20835 at 4283.616 kb on - strand, within ABIE41_RS20835 at 4283.905 kb on + strand, within ABIE41_RS20835 at 4283.958 kb on - strand, within ABIE41_RS20835 at 4283.958 kb on - strand, within ABIE41_RS20835 at 4284.041 kb on + strand, within ABIE41_RS20835 at 4284.041 kb on + strand, within ABIE41_RS20835 at 4284.041 kb on + strand, within ABIE41_RS20835 at 4284.042 kb on - strand, within ABIE41_RS20835 at 4284.042 kb on - strand, within ABIE41_RS20835 at 4284.042 kb on - strand, within ABIE41_RS20835 at 4284.078 kb on + strand, within ABIE41_RS20835 at 4284.079 kb on - strand, within ABIE41_RS20835 at 4284.079 kb on - strand, within ABIE41_RS20835 at 4284.079 kb on - strand, within ABIE41_RS20835 at 4284.147 kb on - strand, within ABIE41_RS20835 at 4284.147 kb on - strand, within ABIE41_RS20835 at 4284.152 kb on + strand, within ABIE41_RS20835 at 4284.153 kb on - strand, within ABIE41_RS20835 at 4284.153 kb on - strand, within ABIE41_RS20835 at 4284.153 kb on - strand, within ABIE41_RS20835 at 4284.153 kb on - strand, within ABIE41_RS20835 at 4284.367 kb on - strand at 4284.528 kb on + strand, within ABIE41_RS20840 at 4284.528 kb on + strand, within ABIE41_RS20840 at 4284.529 kb on - strand, within ABIE41_RS20840 at 4284.529 kb on - strand, within ABIE41_RS20840 at 4284.547 kb on + strand, within ABIE41_RS20840 at 4284.555 kb on + strand, within ABIE41_RS20840 at 4284.556 kb on - strand, within ABIE41_RS20840 at 4284.556 kb on - strand, within ABIE41_RS20840 at 4284.556 kb on - strand, within ABIE41_RS20840 at 4284.556 kb on - strand, within ABIE41_RS20840 at 4285.036 kb on + strand at 4285.036 kb on + strand at 4285.037 kb on - strand at 4285.037 kb on - strand at 4285.343 kb on - strand, within ABIE41_RS20845
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.2709965 mM remove 4,282,963 + +0.6 4,283,103 - -0.7 4,283,103 - -0.9 4,283,150 + ABIE41_RS20835 0.13 +1.0 4,283,150 + ABIE41_RS20835 0.13 -0.7 4,283,150 + ABIE41_RS20835 0.13 +3.0 4,283,151 - ABIE41_RS20835 0.13 +0.3 4,283,534 + ABIE41_RS20835 0.41 +1.6 4,283,534 + ABIE41_RS20835 0.41 +1.7 4,283,534 + ABIE41_RS20835 0.41 +0.3 4,283,534 + ABIE41_RS20835 0.41 +0.3 4,283,534 + ABIE41_RS20835 0.41 -1.6 4,283,534 + ABIE41_RS20835 0.41 +1.7 4,283,534 + ABIE41_RS20835 0.41 -0.4 4,283,535 - ABIE41_RS20835 0.41 +0.2 4,283,546 + ABIE41_RS20835 0.42 +0.1 4,283,546 + ABIE41_RS20835 0.42 -0.1 4,283,547 - ABIE41_RS20835 0.42 +0.0 4,283,547 - ABIE41_RS20835 0.42 +0.5 4,283,615 + ABIE41_RS20835 0.47 -0.2 4,283,616 - ABIE41_RS20835 0.47 +0.0 4,283,616 - ABIE41_RS20835 0.47 -1.2 4,283,905 + ABIE41_RS20835 0.68 -0.9 4,283,958 - ABIE41_RS20835 0.72 -1.9 4,283,958 - ABIE41_RS20835 0.72 +0.2 4,284,041 + ABIE41_RS20835 0.78 -0.0 4,284,041 + ABIE41_RS20835 0.78 +1.3 4,284,041 + ABIE41_RS20835 0.78 +1.0 4,284,042 - ABIE41_RS20835 0.78 +0.2 4,284,042 - ABIE41_RS20835 0.78 -0.6 4,284,042 - ABIE41_RS20835 0.78 +1.0 4,284,078 + ABIE41_RS20835 0.81 +0.8 4,284,079 - ABIE41_RS20835 0.81 +0.0 4,284,079 - ABIE41_RS20835 0.81 -1.6 4,284,079 - ABIE41_RS20835 0.81 -0.4 4,284,147 - ABIE41_RS20835 0.86 +0.5 4,284,147 - ABIE41_RS20835 0.86 +0.1 4,284,152 + ABIE41_RS20835 0.86 +1.5 4,284,153 - ABIE41_RS20835 0.86 +0.4 4,284,153 - ABIE41_RS20835 0.86 +1.1 4,284,153 - ABIE41_RS20835 0.86 -0.4 4,284,153 - ABIE41_RS20835 0.86 +0.7 4,284,367 - -0.7 4,284,528 + ABIE41_RS20840 0.36 -2.5 4,284,528 + ABIE41_RS20840 0.36 +0.1 4,284,529 - ABIE41_RS20840 0.37 -2.4 4,284,529 - ABIE41_RS20840 0.37 +0.2 4,284,547 + ABIE41_RS20840 0.40 +0.8 4,284,555 + ABIE41_RS20840 0.42 +0.4 4,284,556 - ABIE41_RS20840 0.42 -0.7 4,284,556 - ABIE41_RS20840 0.42 +1.4 4,284,556 - ABIE41_RS20840 0.42 +1.0 4,284,556 - ABIE41_RS20840 0.42 +0.6 4,285,036 + +0.6 4,285,036 + -2.9 4,285,037 - +0.7 4,285,037 - +1.0 4,285,343 - ABIE41_RS20845 0.22 +0.0
Or see this region's nucleotide sequence