Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS07235 and ABIE41_RS07240 are separated by 20 nucleotides ABIE41_RS07240 and ABIE41_RS07245 are separated by 25 nucleotides ABIE41_RS07245 and ABIE41_RS07250 overlap by 4 nucleotides
ABIE41_RS07235: ABIE41_RS07235 - adenylate/guanylate cyclase domain-containing protein, at 1,491,812 to 1,493,734
_RS07235
ABIE41_RS07240: ABIE41_RS07240 - hypothetical protein, at 1,493,755 to 1,494,357
_RS07240
ABIE41_RS07245: ABIE41_RS07245 - class I SAM-dependent methyltransferase, at 1,494,383 to 1,495,045
_RS07245
ABIE41_RS07250: ABIE41_RS07250 - glycosyltransferase, at 1,495,042 to 1,496,284
_RS07250
Position (kb)
1494
1495
1496 Strain fitness (log2 ratio)
-2
-1
0
1 at 1493.396 kb on + strand, within ABIE41_RS07235 at 1493.397 kb on - strand, within ABIE41_RS07235 at 1493.397 kb on - strand, within ABIE41_RS07235 at 1494.067 kb on - strand, within ABIE41_RS07240 at 1494.344 kb on + strand at 1494.344 kb on + strand at 1494.344 kb on + strand at 1494.344 kb on + strand at 1494.345 kb on - strand at 1494.345 kb on - strand at 1494.345 kb on - strand at 1494.345 kb on - strand at 1494.345 kb on - strand at 1494.413 kb on + strand at 1494.413 kb on + strand at 1494.413 kb on + strand at 1494.413 kb on + strand at 1494.413 kb on + strand at 1494.413 kb on + strand at 1494.413 kb on + strand at 1494.413 kb on + strand at 1494.413 kb on + strand at 1494.413 kb on + strand at 1494.413 kb on + strand at 1494.413 kb on + strand at 1494.413 kb on + strand at 1494.414 kb on - strand at 1494.414 kb on - strand at 1494.414 kb on - strand at 1494.414 kb on - strand at 1494.414 kb on - strand at 1494.414 kb on - strand at 1494.414 kb on - strand at 1494.614 kb on + strand, within ABIE41_RS07245 at 1494.614 kb on + strand, within ABIE41_RS07245 at 1494.615 kb on - strand, within ABIE41_RS07245 at 1494.893 kb on + strand, within ABIE41_RS07245 at 1494.905 kb on + strand, within ABIE41_RS07245 at 1494.906 kb on - strand, within ABIE41_RS07245 at 1494.906 kb on - strand, within ABIE41_RS07245 at 1494.906 kb on - strand, within ABIE41_RS07245 at 1494.906 kb on - strand, within ABIE41_RS07245 at 1495.013 kb on + strand at 1495.013 kb on + strand at 1495.013 kb on + strand at 1495.013 kb on + strand at 1495.013 kb on + strand at 1495.013 kb on + strand at 1495.014 kb on - strand at 1495.014 kb on - strand at 1495.014 kb on - strand at 1495.014 kb on - strand at 1495.217 kb on + strand, within ABIE41_RS07250 at 1495.217 kb on + strand, within ABIE41_RS07250 at 1495.218 kb on - strand, within ABIE41_RS07250 at 1495.222 kb on + strand, within ABIE41_RS07250 at 1495.222 kb on + strand, within ABIE41_RS07250 at 1495.222 kb on + strand, within ABIE41_RS07250 at 1495.222 kb on + strand, within ABIE41_RS07250 at 1495.222 kb on + strand, within ABIE41_RS07250 at 1495.222 kb on + strand, within ABIE41_RS07250 at 1495.223 kb on - strand, within ABIE41_RS07250 at 1495.223 kb on - strand, within ABIE41_RS07250 at 1495.223 kb on - strand, within ABIE41_RS07250 at 1495.223 kb on - strand, within ABIE41_RS07250 at 1495.223 kb on - strand, within ABIE41_RS07250 at 1495.223 kb on - strand, within ABIE41_RS07250 at 1495.234 kb on + strand, within ABIE41_RS07250 at 1495.235 kb on - strand, within ABIE41_RS07250 at 1495.235 kb on - strand, within ABIE41_RS07250 at 1495.235 kb on - strand, within ABIE41_RS07250 at 1495.357 kb on - strand, within ABIE41_RS07250 at 1495.429 kb on + strand, within ABIE41_RS07250 at 1495.429 kb on + strand, within ABIE41_RS07250 at 1495.429 kb on + strand, within ABIE41_RS07250 at 1495.429 kb on + strand, within ABIE41_RS07250 at 1495.429 kb on + strand, within ABIE41_RS07250 at 1495.430 kb on - strand, within ABIE41_RS07250 at 1495.430 kb on - strand, within ABIE41_RS07250 at 1495.430 kb on - strand, within ABIE41_RS07250 at 1495.430 kb on - strand, within ABIE41_RS07250 at 1495.430 kb on - strand, within ABIE41_RS07250 at 1495.430 kb on - strand, within ABIE41_RS07250 at 1495.452 kb on - strand, within ABIE41_RS07250 at 1495.452 kb on - strand, within ABIE41_RS07250 at 1495.460 kb on - strand, within ABIE41_RS07250 at 1495.643 kb on + strand, within ABIE41_RS07250 at 1495.643 kb on + strand, within ABIE41_RS07250 at 1495.644 kb on - strand, within ABIE41_RS07250 at 1495.870 kb on - strand, within ABIE41_RS07250
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM remove 1,493,396 + ABIE41_RS07235 0.82 -0.4 1,493,397 - ABIE41_RS07235 0.82 -0.0 1,493,397 - ABIE41_RS07235 0.82 +1.3 1,494,067 - ABIE41_RS07240 0.52 +0.5 1,494,344 + -0.1 1,494,344 + -0.3 1,494,344 + -0.2 1,494,344 + -0.3 1,494,345 - +0.0 1,494,345 - -0.5 1,494,345 - -0.4 1,494,345 - +0.2 1,494,345 - -0.3 1,494,413 + -0.9 1,494,413 + -0.9 1,494,413 + -0.2 1,494,413 + +1.0 1,494,413 + -0.8 1,494,413 + -0.3 1,494,413 + -0.8 1,494,413 + +0.2 1,494,413 + +0.0 1,494,413 + +0.1 1,494,413 + +0.6 1,494,413 + +0.3 1,494,413 + +0.5 1,494,414 - -1.0 1,494,414 - +0.1 1,494,414 - +0.2 1,494,414 - +0.0 1,494,414 - +1.0 1,494,414 - -0.4 1,494,414 - -0.5 1,494,614 + ABIE41_RS07245 0.35 +0.4 1,494,614 + ABIE41_RS07245 0.35 +0.4 1,494,615 - ABIE41_RS07245 0.35 -0.1 1,494,893 + ABIE41_RS07245 0.77 -0.3 1,494,905 + ABIE41_RS07245 0.79 -1.5 1,494,906 - ABIE41_RS07245 0.79 +1.2 1,494,906 - ABIE41_RS07245 0.79 +1.2 1,494,906 - ABIE41_RS07245 0.79 +0.4 1,494,906 - ABIE41_RS07245 0.79 -0.3 1,495,013 + +0.3 1,495,013 + -0.3 1,495,013 + +0.1 1,495,013 + +0.6 1,495,013 + +0.0 1,495,013 + +0.2 1,495,014 - +0.1 1,495,014 - +0.7 1,495,014 - -0.9 1,495,014 - -0.0 1,495,217 + ABIE41_RS07250 0.14 -1.3 1,495,217 + ABIE41_RS07250 0.14 -0.8 1,495,218 - ABIE41_RS07250 0.14 -1.0 1,495,222 + ABIE41_RS07250 0.14 +0.2 1,495,222 + ABIE41_RS07250 0.14 +0.7 1,495,222 + ABIE41_RS07250 0.14 -0.2 1,495,222 + ABIE41_RS07250 0.14 -1.0 1,495,222 + ABIE41_RS07250 0.14 -1.5 1,495,222 + ABIE41_RS07250 0.14 -0.4 1,495,223 - ABIE41_RS07250 0.15 -1.0 1,495,223 - ABIE41_RS07250 0.15 -1.5 1,495,223 - ABIE41_RS07250 0.15 -0.4 1,495,223 - ABIE41_RS07250 0.15 -0.6 1,495,223 - ABIE41_RS07250 0.15 -0.2 1,495,223 - ABIE41_RS07250 0.15 +0.4 1,495,234 + ABIE41_RS07250 0.15 +0.1 1,495,235 - ABIE41_RS07250 0.16 +0.2 1,495,235 - ABIE41_RS07250 0.16 -0.5 1,495,235 - ABIE41_RS07250 0.16 -0.3 1,495,357 - ABIE41_RS07250 0.25 -2.3 1,495,429 + ABIE41_RS07250 0.31 -0.3 1,495,429 + ABIE41_RS07250 0.31 +0.4 1,495,429 + ABIE41_RS07250 0.31 -0.2 1,495,429 + ABIE41_RS07250 0.31 +0.3 1,495,429 + ABIE41_RS07250 0.31 -0.4 1,495,430 - ABIE41_RS07250 0.31 -0.6 1,495,430 - ABIE41_RS07250 0.31 -0.3 1,495,430 - ABIE41_RS07250 0.31 +0.9 1,495,430 - ABIE41_RS07250 0.31 -0.1 1,495,430 - ABIE41_RS07250 0.31 +0.0 1,495,430 - ABIE41_RS07250 0.31 +1.4 1,495,452 - ABIE41_RS07250 0.33 -0.7 1,495,452 - ABIE41_RS07250 0.33 -1.1 1,495,460 - ABIE41_RS07250 0.34 -0.5 1,495,643 + ABIE41_RS07250 0.48 +1.4 1,495,643 + ABIE41_RS07250 0.48 -0.4 1,495,644 - ABIE41_RS07250 0.48 -0.7 1,495,870 - ABIE41_RS07250 0.67 +0.4
Or see this region's nucleotide sequence