Experiment: R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE41_RS05965 and ABIE41_RS05970 are separated by 79 nucleotides ABIE41_RS05970 and ABIE41_RS05975 are separated by 18 nucleotides ABIE41_RS05975 and ABIE41_RS05980 are separated by 89 nucleotides ABIE41_RS05980 and ABIE41_RS05985 overlap by 4 nucleotides
ABIE41_RS05965: ABIE41_RS05965 - ABC transporter ATP-binding protein, at 1,230,760 to 1,231,608
_RS05965
ABIE41_RS05970: ABIE41_RS05970 - AMP-binding protein, at 1,231,688 to 1,232,920
_RS05970
ABIE41_RS05975: ABIE41_RS05975 - hypothetical protein, at 1,232,939 to 1,233,130
_RS05975
ABIE41_RS05980: ABIE41_RS05980 - uracil-DNA glycosylase, at 1,233,220 to 1,233,918
_RS05980
ABIE41_RS05985: ABIE41_RS05985 - bifunctional UDP-sugar hydrolase/5'-nucleotidase, at 1,233,915 to 1,235,435
_RS05985
Position (kb) 1231
1232
1233 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1230.844 kb on + strand at 1230.844 kb on + strand at 1230.844 kb on + strand at 1230.844 kb on + strand at 1230.844 kb on + strand at 1230.844 kb on + strand at 1230.844 kb on + strand at 1230.844 kb on + strand at 1230.845 kb on - strand, within ABIE41_RS05965 at 1230.845 kb on - strand, within ABIE41_RS05965 at 1230.845 kb on - strand, within ABIE41_RS05965 at 1231.186 kb on + strand, within ABIE41_RS05965 at 1231.186 kb on + strand, within ABIE41_RS05965 at 1231.186 kb on + strand, within ABIE41_RS05965 at 1231.186 kb on + strand, within ABIE41_RS05965 at 1231.186 kb on + strand, within ABIE41_RS05965 at 1231.186 kb on + strand, within ABIE41_RS05965 at 1231.186 kb on + strand, within ABIE41_RS05965 at 1231.186 kb on + strand, within ABIE41_RS05965 at 1231.186 kb on + strand, within ABIE41_RS05965 at 1231.186 kb on + strand, within ABIE41_RS05965 at 1231.187 kb on - strand, within ABIE41_RS05965 at 1231.187 kb on - strand, within ABIE41_RS05965 at 1231.187 kb on - strand, within ABIE41_RS05965 at 1231.234 kb on + strand, within ABIE41_RS05965 at 1231.444 kb on + strand, within ABIE41_RS05965 at 1231.444 kb on + strand, within ABIE41_RS05965 at 1231.444 kb on + strand, within ABIE41_RS05965 at 1231.444 kb on + strand, within ABIE41_RS05965 at 1231.444 kb on + strand, within ABIE41_RS05965 at 1231.444 kb on + strand, within ABIE41_RS05965 at 1231.445 kb on - strand, within ABIE41_RS05965 at 1231.445 kb on - strand, within ABIE41_RS05965 at 1231.445 kb on - strand, within ABIE41_RS05965 at 1231.445 kb on - strand, within ABIE41_RS05965 at 1231.445 kb on - strand, within ABIE41_RS05965 at 1231.450 kb on + strand, within ABIE41_RS05965 at 1231.450 kb on + strand, within ABIE41_RS05965 at 1231.450 kb on + strand, within ABIE41_RS05965 at 1231.450 kb on + strand, within ABIE41_RS05965 at 1231.450 kb on + strand, within ABIE41_RS05965 at 1231.450 kb on + strand, within ABIE41_RS05965 at 1231.450 kb on + strand, within ABIE41_RS05965 at 1231.450 kb on + strand, within ABIE41_RS05965 at 1231.451 kb on - strand, within ABIE41_RS05965 at 1231.451 kb on - strand, within ABIE41_RS05965 at 1231.451 kb on - strand, within ABIE41_RS05965 at 1231.451 kb on - strand, within ABIE41_RS05965 at 1231.451 kb on - strand, within ABIE41_RS05965 at 1231.451 kb on - strand, within ABIE41_RS05965 at 1231.451 kb on - strand, within ABIE41_RS05965 at 1231.451 kb on - strand, within ABIE41_RS05965 at 1231.528 kb on + strand at 1231.529 kb on - strand at 1231.529 kb on - strand at 1231.529 kb on - strand at 1231.579 kb on + strand at 1231.579 kb on + strand at 1231.579 kb on + strand at 1231.579 kb on + strand at 1231.579 kb on + strand at 1231.580 kb on - strand at 1231.580 kb on - strand at 1231.797 kb on - strand at 1231.797 kb on - strand at 1231.797 kb on - strand at 1232.079 kb on - strand, within ABIE41_RS05970 at 1232.079 kb on - strand, within ABIE41_RS05970 at 1232.192 kb on + strand, within ABIE41_RS05970 at 1232.207 kb on + strand, within ABIE41_RS05970 at 1232.357 kb on + strand, within ABIE41_RS05970 at 1232.357 kb on + strand, within ABIE41_RS05970 at 1232.357 kb on + strand, within ABIE41_RS05970 at 1232.357 kb on + strand, within ABIE41_RS05970 at 1232.357 kb on + strand, within ABIE41_RS05970 at 1232.366 kb on + strand, within ABIE41_RS05970 at 1232.367 kb on - strand, within ABIE41_RS05970 at 1232.367 kb on - strand, within ABIE41_RS05970 at 1232.367 kb on - strand, within ABIE41_RS05970 at 1232.397 kb on - strand, within ABIE41_RS05970 at 1232.777 kb on + strand, within ABIE41_RS05970 at 1232.778 kb on - strand, within ABIE41_RS05970 at 1232.778 kb on - strand, within ABIE41_RS05970 at 1232.940 kb on + strand at 1232.940 kb on + strand at 1232.941 kb on - strand at 1233.108 kb on + strand, within ABIE41_RS05975 at 1233.108 kb on + strand, within ABIE41_RS05975 at 1233.108 kb on + strand, within ABIE41_RS05975 at 1233.108 kb on + strand, within ABIE41_RS05975 at 1233.109 kb on - strand, within ABIE41_RS05975 at 1233.109 kb on - strand, within ABIE41_RS05975 at 1233.109 kb on - strand, within ABIE41_RS05975 at 1233.109 kb on - strand, within ABIE41_RS05975 at 1233.109 kb on - strand, within ABIE41_RS05975 at 1233.109 kb on - strand, within ABIE41_RS05975 at 1233.109 kb on - strand, within ABIE41_RS05975 at 1233.189 kb on + strand at 1233.190 kb on - strand at 1233.190 kb on - strand at 1233.190 kb on - strand at 1233.221 kb on + strand at 1233.221 kb on + strand at 1233.221 kb on + strand at 1233.221 kb on + strand at 1233.221 kb on + strand at 1233.222 kb on - strand at 1233.222 kb on - strand at 1233.222 kb on - strand at 1233.650 kb on + strand, within ABIE41_RS05980 at 1233.651 kb on - strand, within ABIE41_RS05980 at 1233.651 kb on - strand, within ABIE41_RS05980 at 1233.689 kb on + strand, within ABIE41_RS05980
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with 6-Pentyl-2H-pyran-2-one 0.2865 mM remove 1,230,844 + -0.4 1,230,844 + +0.1 1,230,844 + -1.4 1,230,844 + -0.7 1,230,844 + -0.0 1,230,844 + +0.7 1,230,844 + -0.5 1,230,844 + -0.2 1,230,845 - ABIE41_RS05965 0.10 -0.4 1,230,845 - ABIE41_RS05965 0.10 +0.5 1,230,845 - ABIE41_RS05965 0.10 +1.6 1,231,186 + ABIE41_RS05965 0.50 +0.6 1,231,186 + ABIE41_RS05965 0.50 +0.6 1,231,186 + ABIE41_RS05965 0.50 -0.2 1,231,186 + ABIE41_RS05965 0.50 -0.8 1,231,186 + ABIE41_RS05965 0.50 +0.7 1,231,186 + ABIE41_RS05965 0.50 -0.4 1,231,186 + ABIE41_RS05965 0.50 +1.4 1,231,186 + ABIE41_RS05965 0.50 -1.2 1,231,186 + ABIE41_RS05965 0.50 +0.7 1,231,186 + ABIE41_RS05965 0.50 -0.4 1,231,187 - ABIE41_RS05965 0.50 -0.4 1,231,187 - ABIE41_RS05965 0.50 +0.0 1,231,187 - ABIE41_RS05965 0.50 +2.4 1,231,234 + ABIE41_RS05965 0.56 -0.3 1,231,444 + ABIE41_RS05965 0.81 -0.2 1,231,444 + ABIE41_RS05965 0.81 -0.5 1,231,444 + ABIE41_RS05965 0.81 -0.5 1,231,444 + ABIE41_RS05965 0.81 +0.4 1,231,444 + ABIE41_RS05965 0.81 -2.5 1,231,444 + ABIE41_RS05965 0.81 +0.0 1,231,445 - ABIE41_RS05965 0.81 +3.0 1,231,445 - ABIE41_RS05965 0.81 -1.2 1,231,445 - ABIE41_RS05965 0.81 -0.4 1,231,445 - ABIE41_RS05965 0.81 +0.2 1,231,445 - ABIE41_RS05965 0.81 -1.0 1,231,450 + ABIE41_RS05965 0.81 +1.4 1,231,450 + ABIE41_RS05965 0.81 -0.0 1,231,450 + ABIE41_RS05965 0.81 +1.5 1,231,450 + ABIE41_RS05965 0.81 -0.1 1,231,450 + ABIE41_RS05965 0.81 -0.5 1,231,450 + ABIE41_RS05965 0.81 +0.4 1,231,450 + ABIE41_RS05965 0.81 -0.6 1,231,450 + ABIE41_RS05965 0.81 +1.0 1,231,451 - ABIE41_RS05965 0.81 +1.3 1,231,451 - ABIE41_RS05965 0.81 +0.4 1,231,451 - ABIE41_RS05965 0.81 +0.1 1,231,451 - ABIE41_RS05965 0.81 +0.1 1,231,451 - ABIE41_RS05965 0.81 +0.6 1,231,451 - ABIE41_RS05965 0.81 +0.2 1,231,451 - ABIE41_RS05965 0.81 -1.9 1,231,451 - ABIE41_RS05965 0.81 +0.9 1,231,528 + -0.7 1,231,529 - +0.2 1,231,529 - -0.8 1,231,529 - -0.5 1,231,579 + +0.4 1,231,579 + -0.0 1,231,579 + -0.6 1,231,579 + -0.1 1,231,579 + -0.3 1,231,580 - +1.0 1,231,580 - +3.3 1,231,797 - -0.7 1,231,797 - +0.8 1,231,797 - -0.4 1,232,079 - ABIE41_RS05970 0.32 +0.9 1,232,079 - ABIE41_RS05970 0.32 -0.3 1,232,192 + ABIE41_RS05970 0.41 +0.6 1,232,207 + ABIE41_RS05970 0.42 +0.2 1,232,357 + ABIE41_RS05970 0.54 +2.3 1,232,357 + ABIE41_RS05970 0.54 -1.2 1,232,357 + ABIE41_RS05970 0.54 +0.0 1,232,357 + ABIE41_RS05970 0.54 -0.1 1,232,357 + ABIE41_RS05970 0.54 +0.8 1,232,366 + ABIE41_RS05970 0.55 +0.3 1,232,367 - ABIE41_RS05970 0.55 +0.9 1,232,367 - ABIE41_RS05970 0.55 -0.7 1,232,367 - ABIE41_RS05970 0.55 -0.2 1,232,397 - ABIE41_RS05970 0.58 +0.4 1,232,777 + ABIE41_RS05970 0.88 -0.4 1,232,778 - ABIE41_RS05970 0.88 -0.3 1,232,778 - ABIE41_RS05970 0.88 -0.1 1,232,940 + -0.1 1,232,940 + -2.1 1,232,941 - -0.7 1,233,108 + ABIE41_RS05975 0.88 -0.3 1,233,108 + ABIE41_RS05975 0.88 +0.0 1,233,108 + ABIE41_RS05975 0.88 +0.2 1,233,108 + ABIE41_RS05975 0.88 +0.2 1,233,109 - ABIE41_RS05975 0.89 +0.7 1,233,109 - ABIE41_RS05975 0.89 -0.2 1,233,109 - ABIE41_RS05975 0.89 -1.1 1,233,109 - ABIE41_RS05975 0.89 -0.1 1,233,109 - ABIE41_RS05975 0.89 -0.3 1,233,109 - ABIE41_RS05975 0.89 -0.5 1,233,109 - ABIE41_RS05975 0.89 -0.3 1,233,189 + -0.3 1,233,190 - -0.5 1,233,190 - -0.1 1,233,190 - -0.5 1,233,221 + +0.0 1,233,221 + +0.3 1,233,221 + -0.2 1,233,221 + -0.3 1,233,221 + -0.2 1,233,222 - +1.8 1,233,222 - -0.4 1,233,222 - +0.1 1,233,650 + ABIE41_RS05980 0.62 +0.4 1,233,651 - ABIE41_RS05980 0.62 -0.0 1,233,651 - ABIE41_RS05980 0.62 -2.4 1,233,689 + ABIE41_RS05980 0.67 -0.8
Or see this region's nucleotide sequence