Experiment: HBM-CucXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recA and pncC are separated by 95 nucleotides pncC and mltB are separated by 670 nucleotides
LU632_RS15735: recA - recombinase RecA, at 2,977,894 to 2,978,961
recA
LU632_RS15740: pncC - nicotinamide-nucleotide amidase, at 2,979,057 to 2,979,551
pncC
LU632_RS15745: mltB - lytic murein transglycosylase B, at 2,980,222 to 2,981,301
mltB
Position (kb)
2979
2980 Strain fitness (log2 ratio)
-1
0
1 at 2978.085 kb on + strand, within recA at 2978.995 kb on - strand at 2978.995 kb on - strand at 2978.995 kb on - strand at 2979.329 kb on - strand, within pncC at 2979.329 kb on - strand, within pncC at 2979.386 kb on - strand, within pncC at 2979.386 kb on - strand, within pncC at 2979.386 kb on - strand, within pncC at 2979.438 kb on - strand, within pncC at 2979.654 kb on + strand at 2979.655 kb on - strand at 2979.659 kb on - strand at 2979.662 kb on + strand at 2979.677 kb on - strand at 2979.682 kb on - strand at 2979.720 kb on - strand at 2979.724 kb on + strand at 2979.732 kb on + strand at 2979.733 kb on - strand at 2979.733 kb on - strand at 2979.804 kb on - strand at 2979.833 kb on - strand at 2979.845 kb on + strand at 2979.861 kb on - strand at 2979.867 kb on - strand at 2979.885 kb on + strand at 2979.886 kb on - strand at 2979.917 kb on + strand at 2979.918 kb on - strand at 2979.964 kb on + strand at 2979.983 kb on - strand at 2980.060 kb on - strand at 2980.103 kb on - strand at 2980.170 kb on - strand at 2980.170 kb on - strand at 2980.172 kb on + strand at 2980.193 kb on + strand at 2980.277 kb on + strand at 2980.293 kb on + strand at 2980.335 kb on - strand, within mltB at 2980.437 kb on + strand, within mltB at 2980.437 kb on + strand, within mltB at 2980.438 kb on - strand, within mltB at 2980.440 kb on - strand, within mltB at 2980.533 kb on - strand, within mltB
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-CucXylem remove 2,978,085 + recA LU632_RS15735 0.18 -0.8 2,978,995 - +1.1 2,978,995 - +0.1 2,978,995 - +1.4 2,979,329 - pncC LU632_RS15740 0.55 -1.1 2,979,329 - pncC LU632_RS15740 0.55 -0.7 2,979,386 - pncC LU632_RS15740 0.66 -0.9 2,979,386 - pncC LU632_RS15740 0.66 -0.4 2,979,386 - pncC LU632_RS15740 0.66 +0.2 2,979,438 - pncC LU632_RS15740 0.77 +0.8 2,979,654 + +0.3 2,979,655 - -0.1 2,979,659 - +0.3 2,979,662 + +0.4 2,979,677 - -0.2 2,979,682 - +0.8 2,979,720 - -0.2 2,979,724 + +0.6 2,979,732 + +0.2 2,979,733 - +0.1 2,979,733 - -0.1 2,979,804 - +0.1 2,979,833 - +0.0 2,979,845 + +0.4 2,979,861 - +0.2 2,979,867 - +0.4 2,979,885 + +1.3 2,979,886 - -0.2 2,979,917 + +0.1 2,979,918 - -0.3 2,979,964 + +0.5 2,979,983 - -0.7 2,980,060 - +1.4 2,980,103 - +0.4 2,980,170 - -0.8 2,980,170 - -0.1 2,980,172 + -1.3 2,980,193 + -0.4 2,980,277 + +1.0 2,980,293 + +1.1 2,980,335 - mltB LU632_RS15745 0.10 +0.4 2,980,437 + mltB LU632_RS15745 0.20 +0.1 2,980,437 + mltB LU632_RS15745 0.20 -0.9 2,980,438 - mltB LU632_RS15745 0.20 +0.4 2,980,440 - mltB LU632_RS15745 0.20 -1.5 2,980,533 - mltB LU632_RS15745 0.29 +0.8
Or see this region's nucleotide sequence