Experiment: HBM-CucXylem
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purE and purK overlap by 4 nucleotides
LU632_RS15245: purE - 5-(carboxyamino)imidazole ribonucleotide mutase, at 2,885,791 to 2,886,300
purE
LU632_RS15250: purK - 5-(carboxyamino)imidazole ribonucleotide synthase, at 2,886,297 to 2,887,379
purK
Position (kb)
2885
2886
2887 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2884.921 kb on + strand at 2884.921 kb on + strand at 2884.921 kb on + strand at 2884.921 kb on + strand at 2885.060 kb on - strand at 2885.185 kb on - strand at 2885.190 kb on + strand at 2885.190 kb on + strand at 2885.190 kb on + strand at 2885.191 kb on - strand at 2885.191 kb on - strand at 2885.191 kb on - strand at 2885.191 kb on - strand at 2885.191 kb on - strand at 2885.191 kb on - strand at 2885.194 kb on - strand at 2885.207 kb on - strand at 2885.392 kb on + strand at 2885.431 kb on - strand at 2885.455 kb on + strand at 2885.552 kb on + strand at 2885.553 kb on - strand at 2885.629 kb on + strand at 2885.713 kb on + strand at 2885.714 kb on - strand at 2885.714 kb on - strand at 2885.714 kb on - strand at 2885.714 kb on - strand at 2885.761 kb on + strand at 2885.761 kb on + strand at 2885.761 kb on + strand at 2885.762 kb on - strand at 2885.938 kb on - strand, within purE at 2886.103 kb on + strand, within purE at 2886.141 kb on + strand, within purE at 2886.386 kb on - strand at 2886.622 kb on + strand, within purK at 2886.662 kb on + strand, within purK at 2886.698 kb on - strand, within purK at 2886.812 kb on + strand, within purK at 2886.813 kb on - strand, within purK at 2886.947 kb on + strand, within purK at 2886.948 kb on - strand, within purK at 2886.948 kb on - strand, within purK at 2886.948 kb on - strand, within purK at 2886.951 kb on + strand, within purK at 2886.951 kb on + strand, within purK at 2886.951 kb on + strand, within purK at 2886.952 kb on - strand, within purK at 2886.983 kb on + strand, within purK at 2886.986 kb on - strand, within purK at 2887.029 kb on - strand, within purK at 2887.145 kb on + strand, within purK at 2887.268 kb on + strand, within purK
Per-strain Table
Position Strand Gene LocusTag Fraction HBM-CucXylem remove 2,884,921 + -0.3 2,884,921 + +1.1 2,884,921 + -0.3 2,884,921 + -0.4 2,885,060 - +0.4 2,885,185 - -0.4 2,885,190 + -0.3 2,885,190 + +0.5 2,885,190 + -0.6 2,885,191 - +0.5 2,885,191 - +1.4 2,885,191 - +0.7 2,885,191 - -0.6 2,885,191 - -0.4 2,885,191 - -0.3 2,885,194 - +0.0 2,885,207 - -0.6 2,885,392 + +0.4 2,885,431 - +0.0 2,885,455 + +2.4 2,885,552 + +0.8 2,885,553 - -0.9 2,885,629 + +0.5 2,885,713 + -0.8 2,885,714 - -3.2 2,885,714 - -2.3 2,885,714 - -0.6 2,885,714 - -1.2 2,885,761 + -2.3 2,885,761 + -1.0 2,885,761 + +0.8 2,885,762 - -1.4 2,885,938 - purE LU632_RS15245 0.29 -2.3 2,886,103 + purE LU632_RS15245 0.61 -3.0 2,886,141 + purE LU632_RS15245 0.69 -3.8 2,886,386 - -0.2 2,886,622 + purK LU632_RS15250 0.30 -1.2 2,886,662 + purK LU632_RS15250 0.34 -2.9 2,886,698 - purK LU632_RS15250 0.37 -0.2 2,886,812 + purK LU632_RS15250 0.48 -1.2 2,886,813 - purK LU632_RS15250 0.48 -0.2 2,886,947 + purK LU632_RS15250 0.60 -0.2 2,886,948 - purK LU632_RS15250 0.60 -0.9 2,886,948 - purK LU632_RS15250 0.60 -2.3 2,886,948 - purK LU632_RS15250 0.60 -0.6 2,886,951 + purK LU632_RS15250 0.60 -1.6 2,886,951 + purK LU632_RS15250 0.60 -1.9 2,886,951 + purK LU632_RS15250 0.60 -4.2 2,886,952 - purK LU632_RS15250 0.60 -1.4 2,886,983 + purK LU632_RS15250 0.63 -2.8 2,886,986 - purK LU632_RS15250 0.64 -1.4 2,887,029 - purK LU632_RS15250 0.68 -0.9 2,887,145 + purK LU632_RS15250 0.78 -0.2 2,887,268 + purK LU632_RS15250 0.90 +1.4
Or see this region's nucleotide sequence